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Relative quantification of the metabolite of aconitine in rat urine by LC-ESI-MS/MS and its application to pharmacokinetics.
Anal Sci. 2012; 28(12):1203-5.AS

Abstract

A sensitive and rapid high-performance liquid chromatographic-tandem mass spectrometric (HPLC-MS/MS) method was developed for the quantitation of the major metabolite of aconitine, 16-O-demethylaconitine, lappaconitine as the internal standard in rat urine. Urine samples were precipitated with acetonitrile/methanol (3:1, v/v). Chromatographic separation was achieved on a Kromasil C18 analytical column. Detection was performed by a selective reaction monitoring (SRM) mode via an electrospray ionization (ESI) source operating in the positive ionization mode. The analytical method was validated in terms of specificity, precision, accuracy, extraction recovery. The intra- and inter-day precisions were less than 11.7%, and the accuracy was less than 14.0% for the analyte. The validated method has been applied to a pharmacokinetic study of 16-O-demethylaconitine in rats, following oral administration of aconitine.

Authors+Show Affiliations

China Electric Power Research Institute, Wuhan 430074, P. R. China.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23232242

Citation

He, Huiwen, and Fei Yan. "Relative Quantification of the Metabolite of Aconitine in Rat Urine By LC-ESI-MS/MS and Its Application to Pharmacokinetics." Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry, vol. 28, no. 12, 2012, pp. 1203-5.
He H, Yan F. Relative quantification of the metabolite of aconitine in rat urine by LC-ESI-MS/MS and its application to pharmacokinetics. Anal Sci. 2012;28(12):1203-5.
He, H., & Yan, F. (2012). Relative quantification of the metabolite of aconitine in rat urine by LC-ESI-MS/MS and its application to pharmacokinetics. Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry, 28(12), 1203-5.
He H, Yan F. Relative Quantification of the Metabolite of Aconitine in Rat Urine By LC-ESI-MS/MS and Its Application to Pharmacokinetics. Anal Sci. 2012;28(12):1203-5. PubMed PMID: 23232242.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Relative quantification of the metabolite of aconitine in rat urine by LC-ESI-MS/MS and its application to pharmacokinetics. AU - He,Huiwen, AU - Yan,Fei, PY - 2012/12/13/entrez PY - 2012/12/13/pubmed PY - 2013/6/1/medline SP - 1203 EP - 5 JF - Analytical sciences : the international journal of the Japan Society for Analytical Chemistry JO - Anal Sci VL - 28 IS - 12 N2 - A sensitive and rapid high-performance liquid chromatographic-tandem mass spectrometric (HPLC-MS/MS) method was developed for the quantitation of the major metabolite of aconitine, 16-O-demethylaconitine, lappaconitine as the internal standard in rat urine. Urine samples were precipitated with acetonitrile/methanol (3:1, v/v). Chromatographic separation was achieved on a Kromasil C18 analytical column. Detection was performed by a selective reaction monitoring (SRM) mode via an electrospray ionization (ESI) source operating in the positive ionization mode. The analytical method was validated in terms of specificity, precision, accuracy, extraction recovery. The intra- and inter-day precisions were less than 11.7%, and the accuracy was less than 14.0% for the analyte. The validated method has been applied to a pharmacokinetic study of 16-O-demethylaconitine in rats, following oral administration of aconitine. SN - 1348-2246 UR - https://www.unboundmedicine.com/medline/citation/23232242/Relative_quantification_of_the_metabolite_of_aconitine_in_rat_urine_by_LC_ESI_MS/MS_and_its_application_to_pharmacokinetics_ L2 - http://japanlinkcenter.org/DN/JST.JSTAGE/analsci/28.1203?lang=en&from=PubMed DB - PRIME DP - Unbound Medicine ER -