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Community-acquired methicillin-resistant Staphylococcus aureus clones circulating in Belgium from 2005 to 2009: changing epidemiology.
Eur J Clin Microbiol Infect Dis 2013; 32(5):613-20EJ

Abstract

The present study reports the evolution of the demographic characteristics and the molecular epidemiology of community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) in Belgium from 2005 to 2009. Four hundred and ten CA-MRSA isolates were prospectively collected and screened for the presence of Panton-Valentin leucocidin (PVL) and toxic shock syndrome toxin 1 (TSST-1) encoding genes, while clinical information were recorded. PVL- and TSST-1-positive isolates were genotyped by pulsed-field gel electrophoresis (PFGE). Staphylococcal cassette chromosome mec (SCCmec) type, spa type and multilocus sequence type (MLST) were determined on representative isolates. One hundred and fifty-nine (39 %) isolates were PVL-positive. PVL-positive isolates were significantly more frequently isolated from skin or soft tissue than PVL-negative isolates, causing mainly subcutaneous abscesses and furuncles. Patients with PVL-positive CA-MRSA were significantly younger than patients with PVL-negative CA-MRSA. Eighty-seven percent of the PVL-positive isolates belonged to a limited number (n = 7) of PFGE types belonging to sequence types (ST) ST80, ST8, ST30, ST5, ST152, ST338 and a new ST, a single-locus variant of ST1. A temporal evolution of the distribution of these PFGE types was observed, characterised by (1) the dissemination of the ST8-SCCmecIV arcA-positive (USA300) genotype and (2) a genetic diversification. Forty-seven (11 %) strains were TSST-1-positive, of which 65 % clustered into four PFGE types, all belonging to ST5. The epidemiology of CA-MRSA in Belgium is changing, as the rapid diffusion of the USA300 clone seems to occur, together with a clonal diversification.

Authors+Show Affiliations

Laboratoire de Référence MRSA-Staphylocoques, Service de Microbiologie, Université Libre de Bruxelles (ULB)-Hôpital Erasme, Brussels, Belgium.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23232976

Citation

Brauner, J, et al. "Community-acquired Methicillin-resistant Staphylococcus Aureus Clones Circulating in Belgium From 2005 to 2009: Changing Epidemiology." European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology, vol. 32, no. 5, 2013, pp. 613-20.
Brauner J, Hallin M, Deplano A, et al. Community-acquired methicillin-resistant Staphylococcus aureus clones circulating in Belgium from 2005 to 2009: changing epidemiology. Eur J Clin Microbiol Infect Dis. 2013;32(5):613-20.
Brauner, J., Hallin, M., Deplano, A., De Mendonça, R., Nonhoff, C., De Ryck, R., ... Denis, O. (2013). Community-acquired methicillin-resistant Staphylococcus aureus clones circulating in Belgium from 2005 to 2009: changing epidemiology. European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology, 32(5), pp. 613-20. doi:10.1007/s10096-012-1784-6.
Brauner J, et al. Community-acquired Methicillin-resistant Staphylococcus Aureus Clones Circulating in Belgium From 2005 to 2009: Changing Epidemiology. Eur J Clin Microbiol Infect Dis. 2013;32(5):613-20. PubMed PMID: 23232976.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Community-acquired methicillin-resistant Staphylococcus aureus clones circulating in Belgium from 2005 to 2009: changing epidemiology. AU - Brauner,J, AU - Hallin,M, AU - Deplano,A, AU - De Mendonça,R, AU - Nonhoff,C, AU - De Ryck,R, AU - Roisin,S, AU - Struelens,M J, AU - Denis,O, Y1 - 2012/12/13/ PY - 2012/09/11/received PY - 2012/11/18/accepted PY - 2012/12/13/entrez PY - 2012/12/13/pubmed PY - 2014/2/6/medline SP - 613 EP - 20 JF - European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology JO - Eur. J. Clin. Microbiol. Infect. Dis. VL - 32 IS - 5 N2 - The present study reports the evolution of the demographic characteristics and the molecular epidemiology of community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) in Belgium from 2005 to 2009. Four hundred and ten CA-MRSA isolates were prospectively collected and screened for the presence of Panton-Valentin leucocidin (PVL) and toxic shock syndrome toxin 1 (TSST-1) encoding genes, while clinical information were recorded. PVL- and TSST-1-positive isolates were genotyped by pulsed-field gel electrophoresis (PFGE). Staphylococcal cassette chromosome mec (SCCmec) type, spa type and multilocus sequence type (MLST) were determined on representative isolates. One hundred and fifty-nine (39 %) isolates were PVL-positive. PVL-positive isolates were significantly more frequently isolated from skin or soft tissue than PVL-negative isolates, causing mainly subcutaneous abscesses and furuncles. Patients with PVL-positive CA-MRSA were significantly younger than patients with PVL-negative CA-MRSA. Eighty-seven percent of the PVL-positive isolates belonged to a limited number (n = 7) of PFGE types belonging to sequence types (ST) ST80, ST8, ST30, ST5, ST152, ST338 and a new ST, a single-locus variant of ST1. A temporal evolution of the distribution of these PFGE types was observed, characterised by (1) the dissemination of the ST8-SCCmecIV arcA-positive (USA300) genotype and (2) a genetic diversification. Forty-seven (11 %) strains were TSST-1-positive, of which 65 % clustered into four PFGE types, all belonging to ST5. The epidemiology of CA-MRSA in Belgium is changing, as the rapid diffusion of the USA300 clone seems to occur, together with a clonal diversification. SN - 1435-4373 UR - https://www.unboundmedicine.com/medline/citation/23232976/Community_acquired_methicillin_resistant_Staphylococcus_aureus_clones_circulating_in_Belgium_from_2005_to_2009:_changing_epidemiology_ L2 - https://dx.doi.org/10.1007/s10096-012-1784-6 DB - PRIME DP - Unbound Medicine ER -