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[Study on HPLC fingerprint of Oldenlandia diffusa].
Zhong Yao Cai. 2012 Jun; 35(6):886-92.ZY

Abstract

OBJECTIVE

To establish the HPLC fingerprint chromatogram of Oldenlandia diffusa coupled with chemometrics means for the quality control of multi-batches of medicinal material.

METHODS

The separation was developed on C18 column(4.6 mm x 250 mm, 5 microm) by gradient elution with acetonitrile-water(both containing 0.1 per thousand (V/V) ocetic acid) as mobile phase at a flow rate of 0.8 mL/min, the detection wavelength at 238 nm and column temperature at 30 degrees C. The HPLC fingerprint chromatogram of Oldenlandia diffusa was set up and the main characteristic peaks were identified by comparing with chemical reference substance. The quality of 22 batches of medicinal material was evaluated by similarity assay as well as principal component analysis (PCA) and cluster analysis.

RESULTS

The established HPLC fingerprint chromatogram of Oldenlandia diffusa was specific, precise, reproducible and stable. 11 peaks were chemically identified. The similarity of 17 batches of Oldenlandia diffusa was obviously higher than 5 batches of adulterants. PCA showed that 17 batches of Oldenlandia diffusa were in a domain and 5 batches of adulterants were far apart from the domain. The cluster analysis of the 22 batches of medicinal material showed that 17 batches of Oldenlandia diffusa were in a cluster while 5 batches of adulterants were excluded. Further cluster analysis was carried out for the quality consistency of 17 batches of Oldenlandia diffusa and accordingly they were devided into 4 clusters.

CONCLUSION

With the combination of chemometrics means, the HPLC fingerprint chromatogram provides a method for evaluation of authenticity and quality control of Oldenlandia diffusa, which is favorable to improve overall quality control of Oldenlandia diffusa.

Authors+Show Affiliations

Chen Y
Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, China. jxzscy@163.com
Yao ZH
No affiliation info available
Dai Y
No affiliation info available
Cheng H
No affiliation info available
Wen LR
No affiliation info available
Zhou GX
No affiliation info available
Yao XS
No affiliation info available

MeSH

Chromatography, High Pressure LiquidCluster AnalysisDrugs, Chinese HerbalIridoidsOldenlandiaPlants, MedicinalPrincipal Component AnalysisQuality ControlReproducibility of Results

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

23236820

Citation

Chen, Yan, et al. "[Study On HPLC Fingerprint of Oldenlandia Diffusa]." Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials, vol. 35, no. 6, 2012, pp. 886-92.
Chen Y, Yao ZH, Dai Y, et al. [Study on HPLC fingerprint of Oldenlandia diffusa]. Zhong Yao Cai. 2012;35(6):886-92.
Chen, Y., Yao, Z. H., Dai, Y., Cheng, H., Wen, L. R., Zhou, G. X., & Yao, X. S. (2012). [Study on HPLC fingerprint of Oldenlandia diffusa]. Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials, 35(6), 886-92.
Chen Y, et al. [Study On HPLC Fingerprint of Oldenlandia Diffusa]. Zhong Yao Cai. 2012;35(6):886-92. PubMed PMID: 23236820.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Study on HPLC fingerprint of Oldenlandia diffusa]. AU - Chen,Yan, AU - Yao,Zhi-Hong, AU - Dai,Yi, AU - Cheng,Hong, AU - Wen,Li-Rong, AU - Zhou,Guang-Xiong, AU - Yao,Xin-Sheng, PY - 2012/12/15/entrez PY - 2012/12/15/pubmed PY - 2013/3/13/medline SP - 886 EP - 92 JF - Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials JO - Zhong Yao Cai VL - 35 IS - 6 N2 - OBJECTIVE: To establish the HPLC fingerprint chromatogram of Oldenlandia diffusa coupled with chemometrics means for the quality control of multi-batches of medicinal material. METHODS: The separation was developed on C18 column(4.6 mm x 250 mm, 5 microm) by gradient elution with acetonitrile-water(both containing 0.1 per thousand (V/V) ocetic acid) as mobile phase at a flow rate of 0.8 mL/min, the detection wavelength at 238 nm and column temperature at 30 degrees C. The HPLC fingerprint chromatogram of Oldenlandia diffusa was set up and the main characteristic peaks were identified by comparing with chemical reference substance. The quality of 22 batches of medicinal material was evaluated by similarity assay as well as principal component analysis (PCA) and cluster analysis. RESULTS: The established HPLC fingerprint chromatogram of Oldenlandia diffusa was specific, precise, reproducible and stable. 11 peaks were chemically identified. The similarity of 17 batches of Oldenlandia diffusa was obviously higher than 5 batches of adulterants. PCA showed that 17 batches of Oldenlandia diffusa were in a domain and 5 batches of adulterants were far apart from the domain. The cluster analysis of the 22 batches of medicinal material showed that 17 batches of Oldenlandia diffusa were in a cluster while 5 batches of adulterants were excluded. Further cluster analysis was carried out for the quality consistency of 17 batches of Oldenlandia diffusa and accordingly they were devided into 4 clusters. CONCLUSION: With the combination of chemometrics means, the HPLC fingerprint chromatogram provides a method for evaluation of authenticity and quality control of Oldenlandia diffusa, which is favorable to improve overall quality control of Oldenlandia diffusa. SN - 1001-4454 UR - https://www.unboundmedicine.com/medline/citation/23236820/[Study_on_HPLC_fingerprint_of_Oldenlandia_diffusa]_ DB - PRIME DP - Unbound Medicine ER -