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[The effect of Banxia Baizhu Tianma decoction on renal protein expression in spontaneously hypertensive rats].
Zhong Yao Cai. 2012 Jun; 35(6):935-9.ZY

Abstract

OBJECTIVE

To study the effect of Banxia Baizhu decoction on the spontaneously hypertensive rats and its possible mechanism.

METHODS

Male SHRs of 6 weeks old were randomly and equally divided into two groups, the control SHR group and the group treated with BXBZTMD, the treated group were treated with BXBZTMD orally for 18 weeks, the control SHR group and normotensive WKY group were fed with the same amount of distilled water. Rats' blood pressure was measured through carotid artery catheterization, and protein of kidney tissues was analyzed by two-dimensional electrophoresis after extraction and purification. Different protein spots expressed significantly which were anlyzed by PDQuest software were then identified by MALDI-TOF/TOF MS. The protein expressions of peroxidase 2 and Cu-Zn superoxide in kidney were determined by Western blot.

RESULTS

Compared SHR with WKY, 12 protein spots were changed. BXBZTMD could adjust kidney protein expression such as Cu-Zn superoxide dismutase, peroxidase 2 and may change kidney protein expression such as the alpha-beta-crystallin, which were closely related to oxidative stress. These discoveries of the target proteins suggested that BXBZTMD had curative effect by anti-oxidative stress.

CONCLUSION

Oxidative stress plays an important role in the process of hypertensive renal injury. BXBZTMD can play a therapeutic role to oxidative stress. The discovery of the target protein provides a theoretical basis for the mechanism of the therapeutic effect of BXBZTMD.

Authors+Show Affiliations

Experiment Center for Teaching and Learning, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

23236830

Citation

Luo, Shan-Shan, et al. "[The Effect of Banxia Baizhu Tianma Decoction On Renal Protein Expression in Spontaneously Hypertensive Rats]." Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials, vol. 35, no. 6, 2012, pp. 935-9.
Luo SS, Jiang JY, Li Y, et al. [The effect of Banxia Baizhu Tianma decoction on renal protein expression in spontaneously hypertensive rats]. Zhong Yao Cai. 2012;35(6):935-9.
Luo, S. S., Jiang, J. Y., Li, Y., Lu, J. F., & Ke, Y. (2012). [The effect of Banxia Baizhu Tianma decoction on renal protein expression in spontaneously hypertensive rats]. Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials, 35(6), 935-9.
Luo SS, et al. [The Effect of Banxia Baizhu Tianma Decoction On Renal Protein Expression in Spontaneously Hypertensive Rats]. Zhong Yao Cai. 2012;35(6):935-9. PubMed PMID: 23236830.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [The effect of Banxia Baizhu Tianma decoction on renal protein expression in spontaneously hypertensive rats]. AU - Luo,Shan-Shan, AU - Jiang,Jia-Ye, AU - Li,Yuan, AU - Lu,Jia-Feng, AU - Ke,Yan, PY - 2012/12/15/entrez PY - 2012/12/15/pubmed PY - 2013/3/13/medline SP - 935 EP - 9 JF - Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials JO - Zhong Yao Cai VL - 35 IS - 6 N2 - OBJECTIVE: To study the effect of Banxia Baizhu decoction on the spontaneously hypertensive rats and its possible mechanism. METHODS: Male SHRs of 6 weeks old were randomly and equally divided into two groups, the control SHR group and the group treated with BXBZTMD, the treated group were treated with BXBZTMD orally for 18 weeks, the control SHR group and normotensive WKY group were fed with the same amount of distilled water. Rats' blood pressure was measured through carotid artery catheterization, and protein of kidney tissues was analyzed by two-dimensional electrophoresis after extraction and purification. Different protein spots expressed significantly which were anlyzed by PDQuest software were then identified by MALDI-TOF/TOF MS. The protein expressions of peroxidase 2 and Cu-Zn superoxide in kidney were determined by Western blot. RESULTS: Compared SHR with WKY, 12 protein spots were changed. BXBZTMD could adjust kidney protein expression such as Cu-Zn superoxide dismutase, peroxidase 2 and may change kidney protein expression such as the alpha-beta-crystallin, which were closely related to oxidative stress. These discoveries of the target proteins suggested that BXBZTMD had curative effect by anti-oxidative stress. CONCLUSION: Oxidative stress plays an important role in the process of hypertensive renal injury. BXBZTMD can play a therapeutic role to oxidative stress. The discovery of the target protein provides a theoretical basis for the mechanism of the therapeutic effect of BXBZTMD. SN - 1001-4454 UR - https://www.unboundmedicine.com/medline/citation/23236830/[The_effect_of_Banxia_Baizhu_Tianma_decoction_on_renal_protein_expression_in_spontaneously_hypertensive_rats]_ L2 - https://medlineplus.gov/highbloodpressure.html DB - PRIME DP - Unbound Medicine ER -