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[Effects of mal62-overexpression on leavening ability of baker's yeast].
Wei Sheng Wu Xue Bao 2012; 52(9):1094-102WS

Abstract

OBJECTIVE

To increase the leavening ability in the lean dough, the maltose utilization ability of baker's yeast was enhanced.

METHODS

A 1.7kb PGK1 promoter and terminator were ligated and inserted into vector Yep-C to give the expression plasmid named Yep-CP. Then a 1.7kb DNA fragment containing the open reading frame and terminator of mal62 gene was amplified from Saccharomyces cerevisiae BY-14 by PCR, and inserted into Yep-CP to generate recombinant plasmid Yep-CPM. To express mal62 gene properly in S. cerevisiae, the recombinant expression plasmids Yep-CPM with copper resistance gene as the selection marker for yeast transformation were introduced into S. cerevisiae BY-14. The resulting yeast transformant BYCPM was screened on YEPD with 4 mmol/L CuSO,and identified by colony PCR. Target protein was detected by qRT-PCR, and the enzyme activities and the leavening ability of the recombinant strain BYCPM were determined to confirm whether functional expression was achieved.

RESULTS

The maximum maltase activity of recombinant strain BYCPM was 15%-52% higher than that of control strain BY-C. Leavening ability and specific leavening ability of recombinant strain BYCPM were 40% and 5.6% higher than that of the control strain BY-C, respectively.

CONCLUSION

The mal62-overexpression was an effective way of increasing the maltase activity and the ability of glucose depression in the baker's yeast. Moreover, recombinant strain BYCPM could further enhance the leavening ability and produce more gas while consume lesser carbon source than the control strain.

Authors+Show Affiliations

Tianjin University of Science and Technology College of Biotechnology, Tianjin 300457, China. sunxiaoxisunxiao@163.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

23236843

Citation

Sun, Xi, et al. "[Effects of Mal62-overexpression On Leavening Ability of Baker's Yeast]." Wei Sheng Wu Xue Bao = Acta Microbiologica Sinica, vol. 52, no. 9, 2012, pp. 1094-102.
Sun X, Zhang C, Dong J, et al. [Effects of mal62-overexpression on leavening ability of baker's yeast]. Wei Sheng Wu Xue Bao. 2012;52(9):1094-102.
Sun, X., Zhang, C., Dong, J., Wang, G., Wu, M., & Xiao, D. (2012). [Effects of mal62-overexpression on leavening ability of baker's yeast]. Wei Sheng Wu Xue Bao = Acta Microbiologica Sinica, 52(9), pp. 1094-102.
Sun X, et al. [Effects of Mal62-overexpression On Leavening Ability of Baker's Yeast]. Wei Sheng Wu Xue Bao. 2012 Sep 4;52(9):1094-102. PubMed PMID: 23236843.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of mal62-overexpression on leavening ability of baker's yeast]. AU - Sun,Xi, AU - Zhang,Cuiying, AU - Dong,Jian, AU - Wang,Guanglu, AU - Wu,Mingyue, AU - Xiao,Dongguang, PY - 2012/12/15/entrez PY - 2012/12/15/pubmed PY - 2013/1/9/medline SP - 1094 EP - 102 JF - Wei sheng wu xue bao = Acta microbiologica Sinica JO - Wei Sheng Wu Xue Bao VL - 52 IS - 9 N2 - OBJECTIVE: To increase the leavening ability in the lean dough, the maltose utilization ability of baker's yeast was enhanced. METHODS: A 1.7kb PGK1 promoter and terminator were ligated and inserted into vector Yep-C to give the expression plasmid named Yep-CP. Then a 1.7kb DNA fragment containing the open reading frame and terminator of mal62 gene was amplified from Saccharomyces cerevisiae BY-14 by PCR, and inserted into Yep-CP to generate recombinant plasmid Yep-CPM. To express mal62 gene properly in S. cerevisiae, the recombinant expression plasmids Yep-CPM with copper resistance gene as the selection marker for yeast transformation were introduced into S. cerevisiae BY-14. The resulting yeast transformant BYCPM was screened on YEPD with 4 mmol/L CuSO,and identified by colony PCR. Target protein was detected by qRT-PCR, and the enzyme activities and the leavening ability of the recombinant strain BYCPM were determined to confirm whether functional expression was achieved. RESULTS: The maximum maltase activity of recombinant strain BYCPM was 15%-52% higher than that of control strain BY-C. Leavening ability and specific leavening ability of recombinant strain BYCPM were 40% and 5.6% higher than that of the control strain BY-C, respectively. CONCLUSION: The mal62-overexpression was an effective way of increasing the maltase activity and the ability of glucose depression in the baker's yeast. Moreover, recombinant strain BYCPM could further enhance the leavening ability and produce more gas while consume lesser carbon source than the control strain. SN - 0001-6209 UR - https://www.unboundmedicine.com/medline/citation/23236843/[Effects_of_mal62_overexpression_on_leavening_ability_of_baker's_yeast]_ L2 - http://www.yeastgenome.org/reference/23236843 DB - PRIME DP - Unbound Medicine ER -