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Antigens of Coccidioides posadasii as an important tool for the immunodiagnosis of coccidioidomycosis.
Mycopathologia. 2013 Feb; 175(1-2):25-32.M

Abstract

Serologic diagnosis has been presented as a safe alternative for coccidioidomycosis. However, commercial kits based on coccidioidal antibodies available in the USA are considered too expensive for laboratories outside that country. In this study, we describe the preparation of antigens for detection of human coccidioidal antibodies by the immunodiffusion test (ID) and enzyme immunoassay (EIA). Antigens were tested against serum samples from patients with coccidioidomycosis, histoplasmosis and paracoccidioidomycosis, as well as healthy individuals. The highest reactivity in the ID tests was seen in the F0-90 antigen. In the EIAs, the best results were obtained with the F60-90 antigen. None of the serum samples from healthy individuals were recognized by any of the antigen extracts tested by ID or EIA. In conclusion, the F0-90 and F60-90 antigens have the potential to be commercially employed in presumptive diagnosis of coccidioidomycosis by ID or EIA, respectively. The tests could improve serological diagnosis of coccidioidomycosis in South America.

Authors+Show Affiliations

Postgraduate Program in Medical Microbiology, Department of Pathology and Legal Medicine, Federal University of Ceará, Fortaleza, Ceara, Brazil. rossanacordeiro@ufc.brNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23242703

Citation

de Aguiar Cordeiro, Rossana, et al. "Antigens of Coccidioides Posadasii as an Important Tool for the Immunodiagnosis of Coccidioidomycosis." Mycopathologia, vol. 175, no. 1-2, 2013, pp. 25-32.
de Aguiar Cordeiro R, Patoilo KR, Praciano SB, et al. Antigens of Coccidioides posadasii as an important tool for the immunodiagnosis of coccidioidomycosis. Mycopathologia. 2013;175(1-2):25-32.
de Aguiar Cordeiro, R., Patoilo, K. R., Praciano, S. B., Medrano, D. J., de Farias Marques, F. J., Martins, L. M., Eulalio, K. D., de Deus Filho, A., Cavalvanti, M. d. o. . A., Fechine, M. A., Brilhante, R. S., de Camargo, Z. P., Rocha, M. F., & Sidrim, J. J. (2013). Antigens of Coccidioides posadasii as an important tool for the immunodiagnosis of coccidioidomycosis. Mycopathologia, 175(1-2), 25-32. https://doi.org/10.1007/s11046-012-9604-1
de Aguiar Cordeiro R, et al. Antigens of Coccidioides Posadasii as an Important Tool for the Immunodiagnosis of Coccidioidomycosis. Mycopathologia. 2013;175(1-2):25-32. PubMed PMID: 23242703.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antigens of Coccidioides posadasii as an important tool for the immunodiagnosis of coccidioidomycosis. AU - de Aguiar Cordeiro,Rossana, AU - Patoilo,Kharla Rabelo Nobre, AU - Praciano,Silviane Bandeira, AU - Medrano,Delia Jessica Astete, AU - de Farias Marques,Francisca Jakelyne, AU - Martins,Liline Maria Soares, AU - Eulalio,Kelsen Dantas, AU - de Deus Filho,Antônio, AU - Cavalvanti,Maria do Amparo Salmito, AU - Fechine,Maria Auxiliadora Bezerra, AU - Brilhante,Raimunda Samia Nogueira, AU - de Camargo,Zoilo Pires, AU - Rocha,Marcos Fábio Gadelha, AU - Sidrim,José Júlio Costa, Y1 - 2012/12/14/ PY - 2012/09/24/received PY - 2012/11/21/accepted PY - 2012/12/18/entrez PY - 2012/12/18/pubmed PY - 2013/7/3/medline SP - 25 EP - 32 JF - Mycopathologia JO - Mycopathologia VL - 175 IS - 1-2 N2 - Serologic diagnosis has been presented as a safe alternative for coccidioidomycosis. However, commercial kits based on coccidioidal antibodies available in the USA are considered too expensive for laboratories outside that country. In this study, we describe the preparation of antigens for detection of human coccidioidal antibodies by the immunodiffusion test (ID) and enzyme immunoassay (EIA). Antigens were tested against serum samples from patients with coccidioidomycosis, histoplasmosis and paracoccidioidomycosis, as well as healthy individuals. The highest reactivity in the ID tests was seen in the F0-90 antigen. In the EIAs, the best results were obtained with the F60-90 antigen. None of the serum samples from healthy individuals were recognized by any of the antigen extracts tested by ID or EIA. In conclusion, the F0-90 and F60-90 antigens have the potential to be commercially employed in presumptive diagnosis of coccidioidomycosis by ID or EIA, respectively. The tests could improve serological diagnosis of coccidioidomycosis in South America. SN - 1573-0832 UR - https://www.unboundmedicine.com/medline/citation/23242703/Antigens_of_Coccidioides_posadasii_as_an_important_tool_for_the_immunodiagnosis_of_coccidioidomycosis_ L2 - https://doi.org/10.1007/s11046-012-9604-1 DB - PRIME DP - Unbound Medicine ER -