Tags

Type your tag names separated by a space and hit enter

In vitro toxicity of Stearoyl-poly(glycerol adipate) nanoparticles.
J Appl Biomater Funct Mater. 2012; 10(3):163-9.JA

Abstract

PURPOSE

Poly(glycerol adipate) (PGA)-based nanoparticles are promising carriers for drug delivery with a wide range of available structures. The biodegradable polymer with pendant free hydroxyl groups can be diversely functionalized. In this study, the toxicity of different Stearoyl-PGA nanoparticles with respect to erythrocytes and HepG2 cells was assessed. These cells are crucial test systems for intravenously injected biomedical particles.

METHODS

For this work, a series of PGA polyesters with 0, 20, 50 and 65 mol% of converted hydroxyl groups was synthesized with stearic acid (PGABB, S20, S50, S65). Nanoparticles were prepared with these polymers by an optimized nanoprecipitation method. Physicochemical characterization was performed by photon correlation spectroscopy and zeta potential measurement. Cell compatibility was studied by a hemolysis assay with separated red blood cells as well as a QBlue viability test and a modified LDH cytotoxicity assay with HepG2 cells.

RESULTS AND CONCLUSIONS

Different self-stabilizing nanoparticles with narrow size distributions in the range of 100-140 nm were prepared. All tested nanoparticle samples were nontoxic for HepG2 cells. In fact, increased metabolic activity and proliferation was observed after 24 h incubation with the Stearoyl-PGA particles. Apart from PGAS20, all samples did not show any hemolytic effect. Hemolysis of PGAS20 particles could be considerably decreased by adding Poloxamer 188 during the preparation process.

Authors+Show Affiliations

Institute of Pharmacy, Department of Pharmaceutical Technology and Biopharmacy, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23242880

Citation

Weiss, Verena M., et al. "In Vitro Toxicity of Stearoyl-poly(glycerol Adipate) Nanoparticles." Journal of Applied Biomaterials & Functional Materials, vol. 10, no. 3, 2012, pp. 163-9.
Weiss VM, Naolou T, Groth T, et al. In vitro toxicity of Stearoyl-poly(glycerol adipate) nanoparticles. J Appl Biomater Funct Mater. 2012;10(3):163-9.
Weiss, V. M., Naolou, T., Groth, T., Kressler, J., & Mäder, K. (2012). In vitro toxicity of Stearoyl-poly(glycerol adipate) nanoparticles. Journal of Applied Biomaterials & Functional Materials, 10(3), 163-9. https://doi.org/10.5301/JABFM.2012.10294
Weiss VM, et al. In Vitro Toxicity of Stearoyl-poly(glycerol Adipate) Nanoparticles. J Appl Biomater Funct Mater. 2012;10(3):163-9. PubMed PMID: 23242880.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro toxicity of Stearoyl-poly(glycerol adipate) nanoparticles. AU - Weiss,Verena M, AU - Naolou,Toufik, AU - Groth,Thomas, AU - Kressler,Jörg, AU - Mäder,Karsten, PY - 2012/10/05/accepted PY - 2012/12/18/entrez PY - 2012/12/18/pubmed PY - 2013/9/26/medline SP - 163 EP - 9 JF - Journal of applied biomaterials & functional materials JO - J Appl Biomater Funct Mater VL - 10 IS - 3 N2 - PURPOSE: Poly(glycerol adipate) (PGA)-based nanoparticles are promising carriers for drug delivery with a wide range of available structures. The biodegradable polymer with pendant free hydroxyl groups can be diversely functionalized. In this study, the toxicity of different Stearoyl-PGA nanoparticles with respect to erythrocytes and HepG2 cells was assessed. These cells are crucial test systems for intravenously injected biomedical particles. METHODS: For this work, a series of PGA polyesters with 0, 20, 50 and 65 mol% of converted hydroxyl groups was synthesized with stearic acid (PGABB, S20, S50, S65). Nanoparticles were prepared with these polymers by an optimized nanoprecipitation method. Physicochemical characterization was performed by photon correlation spectroscopy and zeta potential measurement. Cell compatibility was studied by a hemolysis assay with separated red blood cells as well as a QBlue viability test and a modified LDH cytotoxicity assay with HepG2 cells. RESULTS AND CONCLUSIONS: Different self-stabilizing nanoparticles with narrow size distributions in the range of 100-140 nm were prepared. All tested nanoparticle samples were nontoxic for HepG2 cells. In fact, increased metabolic activity and proliferation was observed after 24 h incubation with the Stearoyl-PGA particles. Apart from PGAS20, all samples did not show any hemolytic effect. Hemolysis of PGAS20 particles could be considerably decreased by adding Poloxamer 188 during the preparation process. SN - 2280-8000 UR - https://www.unboundmedicine.com/medline/citation/23242880/In_vitro_toxicity_of_Stearoyl_poly_glycerol_adipate__nanoparticles_ L2 - http://journals.sagepub.com/doi/full/10.5301/JABFM.2012.10294?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -