Tags

Type your tag names separated by a space and hit enter

Expression of the chimeric receptor between the chitin elicitor receptor CEBiP and the receptor-like protein kinase Pi-d2 leads to enhanced responses to the chitin elicitor and disease resistance against Magnaporthe oryzae in rice.
Plant Mol Biol. 2013 Feb; 81(3):287-95.PM

Abstract

We previously reported that rice plants expressing the chimeric receptor consisting of rice chitin oligosaccharides binding protein (CEBiP) and the intracellular protein kinase region of Xa21, which confers resistance to rice bacterial blight, showed enhanced cellular responses to a chitin elicitor N-acetylchitoheptaose and increased resistance to the rice blast fungus Magnaporthe oryzae. Here, we investigated whether CEBiP fused with another type of receptor-like protein kinase (RLK) also functions as a chimeric receptor. Fusion proteins CRPis consist of CEBiP and the intracellular protein kinase region of a true resistance gene Pi-d2. Transgenic rice expressing a CRPi showed enhanced cellular responses specifically to N-acetylchitoheptaose in cultured cells and increased levels of disease resistance against M. oryzae in plants. These responses depended on the amino acid sequences predicted to be essential for the protein kinase activity of CRPi. The structure of the transmembrane domain in CRPi affected the protein accumulation, cellular responses, and disease resistance in transgenic rice. These results suggest that the chimeric receptor consisting of CEBiP and Pi-d2 functions as a receptor for chitin oligosaccharides and CEBiP-based chimeric receptors fused with other RLKs may also act as functional receptors.

Authors+Show Affiliations

Genetically Modified Organism Research Center, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23242918

Citation

Kouzai, Yusuke, et al. "Expression of the Chimeric Receptor Between the Chitin Elicitor Receptor CEBiP and the Receptor-like Protein Kinase Pi-d2 Leads to Enhanced Responses to the Chitin Elicitor and Disease Resistance Against Magnaporthe Oryzae in Rice." Plant Molecular Biology, vol. 81, no. 3, 2013, pp. 287-95.
Kouzai Y, Kaku H, Shibuya N, et al. Expression of the chimeric receptor between the chitin elicitor receptor CEBiP and the receptor-like protein kinase Pi-d2 leads to enhanced responses to the chitin elicitor and disease resistance against Magnaporthe oryzae in rice. Plant Mol Biol. 2013;81(3):287-95.
Kouzai, Y., Kaku, H., Shibuya, N., Minami, E., & Nishizawa, Y. (2013). Expression of the chimeric receptor between the chitin elicitor receptor CEBiP and the receptor-like protein kinase Pi-d2 leads to enhanced responses to the chitin elicitor and disease resistance against Magnaporthe oryzae in rice. Plant Molecular Biology, 81(3), 287-95. https://doi.org/10.1007/s11103-012-9998-7
Kouzai Y, et al. Expression of the Chimeric Receptor Between the Chitin Elicitor Receptor CEBiP and the Receptor-like Protein Kinase Pi-d2 Leads to Enhanced Responses to the Chitin Elicitor and Disease Resistance Against Magnaporthe Oryzae in Rice. Plant Mol Biol. 2013;81(3):287-95. PubMed PMID: 23242918.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of the chimeric receptor between the chitin elicitor receptor CEBiP and the receptor-like protein kinase Pi-d2 leads to enhanced responses to the chitin elicitor and disease resistance against Magnaporthe oryzae in rice. AU - Kouzai,Yusuke, AU - Kaku,Hanae, AU - Shibuya,Naoto, AU - Minami,Eiichi, AU - Nishizawa,Yoko, Y1 - 2012/12/16/ PY - 2012/07/18/received PY - 2012/12/06/accepted PY - 2012/12/18/entrez PY - 2012/12/18/pubmed PY - 2013/3/15/medline SP - 287 EP - 95 JF - Plant molecular biology JO - Plant Mol Biol VL - 81 IS - 3 N2 - We previously reported that rice plants expressing the chimeric receptor consisting of rice chitin oligosaccharides binding protein (CEBiP) and the intracellular protein kinase region of Xa21, which confers resistance to rice bacterial blight, showed enhanced cellular responses to a chitin elicitor N-acetylchitoheptaose and increased resistance to the rice blast fungus Magnaporthe oryzae. Here, we investigated whether CEBiP fused with another type of receptor-like protein kinase (RLK) also functions as a chimeric receptor. Fusion proteins CRPis consist of CEBiP and the intracellular protein kinase region of a true resistance gene Pi-d2. Transgenic rice expressing a CRPi showed enhanced cellular responses specifically to N-acetylchitoheptaose in cultured cells and increased levels of disease resistance against M. oryzae in plants. These responses depended on the amino acid sequences predicted to be essential for the protein kinase activity of CRPi. The structure of the transmembrane domain in CRPi affected the protein accumulation, cellular responses, and disease resistance in transgenic rice. These results suggest that the chimeric receptor consisting of CEBiP and Pi-d2 functions as a receptor for chitin oligosaccharides and CEBiP-based chimeric receptors fused with other RLKs may also act as functional receptors. SN - 1573-5028 UR - https://www.unboundmedicine.com/medline/citation/23242918/Expression_of_the_chimeric_receptor_between_the_chitin_elicitor_receptor_CEBiP_and_the_receptor_like_protein_kinase_Pi_d2_leads_to_enhanced_responses_to_the_chitin_elicitor_and_disease_resistance_against_Magnaporthe_oryzae_in_rice_ L2 - https://doi.org/10.1007/s11103-012-9998-7 DB - PRIME DP - Unbound Medicine ER -