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Hormetic/cytotoxic effects of Nigella sativa seed alcoholic and aqueous extracts on MCF-7 breast cancer cells alone or in combination with doxorubicin.
Cell Biochem Biophys. 2013 Jul; 66(3):451-60.CB

Abstract

In this study, we investigate the possible cytotoxic effects of different Nigella sativa seed extracts on human MCF-7 breast cancer cells and screening the effects of a wide range of extracts concentrations and their application as an adjuvant therapy to doxorubicin. The results obtained showed that the cytotoxic solvent dimethyl sulfoxide can be used for permeation assay in concentration range 697.5-0.341 mmol/ml without affecting the viability of MCF-7 cells. N. sativa lipid extract is cytotoxic to MCF-7 cells with LC50 of 2.72 ± 0.232 mg/ml, while its aqueous extract cytotoxicity exhibited when the applied concentration is high as ≈ 50 mg/ml. The results of this study reveal for the first time that low concentrations of aqueous extract of the seed has a hormetic rather than cytotoxic effect. It is also possible to use cell culture medium or bovine serum to dilute the oil extract for the permeation assay. In conclusion, N. sativa aqueous extract should not be used as antitumor compound by its own. The oil is a promising antitumor compound and its cytotoxicity was greatly enhanced with its nanoemulsion formulation. Antitumor activity of doxorubicin was enhanced, as a function of time, when N. sativa extracts were involved as adjunct therapeutic compounds. Adding doxorubicin to the prepared lipid nanoemulsion has a beneficial impact to their bioactivity. These doxorubicin-N. sativa lipid nanoemulsion are promising and potential therapeutic modality.

Authors+Show Affiliations

Biophysics and Laser Science Unit, Research Institute of Ophthalmology, Giza, Egypt. sheri_sm@yahoo.comNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23242945

Citation

Mahmoud, Sherif S., and Vladmir P. Torchilin. "Hormetic/cytotoxic Effects of Nigella Sativa Seed Alcoholic and Aqueous Extracts On MCF-7 Breast Cancer Cells Alone or in Combination With Doxorubicin." Cell Biochemistry and Biophysics, vol. 66, no. 3, 2013, pp. 451-60.
Mahmoud SS, Torchilin VP. Hormetic/cytotoxic effects of Nigella sativa seed alcoholic and aqueous extracts on MCF-7 breast cancer cells alone or in combination with doxorubicin. Cell Biochem Biophys. 2013;66(3):451-60.
Mahmoud, S. S., & Torchilin, V. P. (2013). Hormetic/cytotoxic effects of Nigella sativa seed alcoholic and aqueous extracts on MCF-7 breast cancer cells alone or in combination with doxorubicin. Cell Biochemistry and Biophysics, 66(3), 451-60. https://doi.org/10.1007/s12013-012-9493-4
Mahmoud SS, Torchilin VP. Hormetic/cytotoxic Effects of Nigella Sativa Seed Alcoholic and Aqueous Extracts On MCF-7 Breast Cancer Cells Alone or in Combination With Doxorubicin. Cell Biochem Biophys. 2013;66(3):451-60. PubMed PMID: 23242945.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hormetic/cytotoxic effects of Nigella sativa seed alcoholic and aqueous extracts on MCF-7 breast cancer cells alone or in combination with doxorubicin. AU - Mahmoud,Sherif S, AU - Torchilin,Vladmir P, PY - 2012/12/18/entrez PY - 2012/12/18/pubmed PY - 2014/3/4/medline SP - 451 EP - 60 JF - Cell biochemistry and biophysics JO - Cell Biochem Biophys VL - 66 IS - 3 N2 - In this study, we investigate the possible cytotoxic effects of different Nigella sativa seed extracts on human MCF-7 breast cancer cells and screening the effects of a wide range of extracts concentrations and their application as an adjuvant therapy to doxorubicin. The results obtained showed that the cytotoxic solvent dimethyl sulfoxide can be used for permeation assay in concentration range 697.5-0.341 mmol/ml without affecting the viability of MCF-7 cells. N. sativa lipid extract is cytotoxic to MCF-7 cells with LC50 of 2.72 ± 0.232 mg/ml, while its aqueous extract cytotoxicity exhibited when the applied concentration is high as ≈ 50 mg/ml. The results of this study reveal for the first time that low concentrations of aqueous extract of the seed has a hormetic rather than cytotoxic effect. It is also possible to use cell culture medium or bovine serum to dilute the oil extract for the permeation assay. In conclusion, N. sativa aqueous extract should not be used as antitumor compound by its own. The oil is a promising antitumor compound and its cytotoxicity was greatly enhanced with its nanoemulsion formulation. Antitumor activity of doxorubicin was enhanced, as a function of time, when N. sativa extracts were involved as adjunct therapeutic compounds. Adding doxorubicin to the prepared lipid nanoemulsion has a beneficial impact to their bioactivity. These doxorubicin-N. sativa lipid nanoemulsion are promising and potential therapeutic modality. SN - 1559-0283 UR - https://www.unboundmedicine.com/medline/citation/23242945/Hormetic/cytotoxic_effects_of_Nigella_sativa_seed_alcoholic_and_aqueous_extracts_on_MCF_7_breast_cancer_cells_alone_or_in_combination_with_doxorubicin_ L2 - https://dx.doi.org/10.1007/s12013-012-9493-4 DB - PRIME DP - Unbound Medicine ER -