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Polymorphism analysis and evaluation of 19 STR loci in the Han population of Southern China.
Ann Hum Biol. 2013 Mar; 40(2):191-6.AH

Abstract

BACKGROUND

The knowledge of allele and genotype frequencies is an essential prerequisite to the use of any human polymorphism in forensic medicine.

AIM

To study the genetic polymorphism and evaluate the 19 STR loci using forensic medicine.

SUBJECTS AND METHODS

Nineteen STR loci, which include D19S433, D5S818, D21S11, D18S51, D6S1043, D3S1358, D13S317, D7S820, D16S539, CSF1PO, Penta D, vWA, D8S1179, TPOX, Penta E, TH01, D12S391, D2S1338 and FGA, were amplified simultaneously with Goldeneye(TM) DNA ID system 20A kit for 1161 unrelated Han individuals in Southern China. The PCR products were separated with the arrayed capillary electrophoresis. The allele frequency distribution and several parameters commonly used in forensic science were statistically analysed.

RESULTS

The observed heterozygosity (Hobs) of these 19 STR loci ranged from 0.6072-0.9070; the expected heterozygosity (Hexp) ranged from 0.6052-0.9117; the power of discrimination (PD) ranged from 0.7836-0.9662; the power of exclusion (PE) ranged from 0.3479-0.8227 for trio paternity cases and 0.1959-0.6988 for duo paternity cases; the polymorphic information content (PIC) ranged from 0.5443-0.9050.

CONCLUSION

The results indicate that 19 STR loci are polymorphic among the Han population in Southern China. This set of polymorphic STR loci is a useful tool in forensic paternity test and anthropological study.

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Authors+Show Affiliations

Tong D
Department of Forensic Biology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510089, PR China.
Chen Y
No affiliation info available
Ou X
No affiliation info available
Chen W
No affiliation info available
Liu S
No affiliation info available
Zhang Y
No affiliation info available
Sun H
No affiliation info available

MeSH

AdultAsian Continental Ancestry GroupChinaElectrophoresis, CapillaryFemaleGene FrequencyGenetics, PopulationGenotypeHumansMaleMicrosatellite RepeatsMiddle AgedPolymerase Chain ReactionYoung Adult

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23244240

Citation

Tong, Dayue, et al. "Polymorphism Analysis and Evaluation of 19 STR Loci in the Han Population of Southern China." Annals of Human Biology, vol. 40, no. 2, 2013, pp. 191-6.
Tong D, Chen Y, Ou X, et al. Polymorphism analysis and evaluation of 19 STR loci in the Han population of Southern China. Ann Hum Biol. 2013;40(2):191-6.
Tong, D., Chen, Y., Ou, X., Chen, W., Liu, S., Zhang, Y., & Sun, H. (2013). Polymorphism analysis and evaluation of 19 STR loci in the Han population of Southern China. Annals of Human Biology, 40(2), 191-6. https://doi.org/10.3109/03014460.2012.750685
Tong D, et al. Polymorphism Analysis and Evaluation of 19 STR Loci in the Han Population of Southern China. Ann Hum Biol. 2013;40(2):191-6. PubMed PMID: 23244240.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Polymorphism analysis and evaluation of 19 STR loci in the Han population of Southern China. AU - Tong,Dayue, AU - Chen,Yong, AU - Ou,Xueling, AU - Chen,Weihong, AU - Liu,Sujuan, AU - Zhang,Yinming, AU - Sun,Hongyu, Y1 - 2012/12/18/ PY - 2012/12/19/entrez PY - 2012/12/19/pubmed PY - 2013/9/6/medline SP - 191 EP - 6 JF - Annals of human biology JO - Ann Hum Biol VL - 40 IS - 2 N2 - BACKGROUND: The knowledge of allele and genotype frequencies is an essential prerequisite to the use of any human polymorphism in forensic medicine. AIM: To study the genetic polymorphism and evaluate the 19 STR loci using forensic medicine. SUBJECTS AND METHODS: Nineteen STR loci, which include D19S433, D5S818, D21S11, D18S51, D6S1043, D3S1358, D13S317, D7S820, D16S539, CSF1PO, Penta D, vWA, D8S1179, TPOX, Penta E, TH01, D12S391, D2S1338 and FGA, were amplified simultaneously with Goldeneye(TM) DNA ID system 20A kit for 1161 unrelated Han individuals in Southern China. The PCR products were separated with the arrayed capillary electrophoresis. The allele frequency distribution and several parameters commonly used in forensic science were statistically analysed. RESULTS: The observed heterozygosity (Hobs) of these 19 STR loci ranged from 0.6072-0.9070; the expected heterozygosity (Hexp) ranged from 0.6052-0.9117; the power of discrimination (PD) ranged from 0.7836-0.9662; the power of exclusion (PE) ranged from 0.3479-0.8227 for trio paternity cases and 0.1959-0.6988 for duo paternity cases; the polymorphic information content (PIC) ranged from 0.5443-0.9050. CONCLUSION: The results indicate that 19 STR loci are polymorphic among the Han population in Southern China. This set of polymorphic STR loci is a useful tool in forensic paternity test and anthropological study. SN - 1464-5033 UR - https://www.unboundmedicine.com/medline/citation/23244240/Polymorphism_analysis_and_evaluation_of_19_STR_loci_in_the_Han_population_of_Southern_China_ L2 - https://www.tandfonline.com/doi/full/10.3109/03014460.2012.750685 DB - PRIME DP - Unbound Medicine ER -