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Anti-inflammatory effect of Sosihotang via inhibition of nuclear factor-κB and mitogen-activated protein kinases signaling pathways in lipopolysaccharide-stimulated RAW 264.7 macrophage cells.
Food Chem Toxicol 2013; 53:343-51FC

Abstract

Sosihotang (SO) is an herbal medication, which has been widely used to treat fever, chill and vomiting due to common cold in east-Asian countries. In this study, to provide insight into the effects of SO on inflammation, we investigated its effect on pro-inflammatory mediator production in RAW 264.7 cells and mouse peritoneal macrophages using lipopolysaccharide (LPS) stimulation. SO significantly inhibited the production of nitric oxide (NO), tumor necrosis factor (TNF)-α and interleukin (IL)-6 as well as gene expression of inducible nitric oxide synthase (iNOS), its synthesizing enzyme. In addition, SO inhibited nuclear factor (NF)-κB activation and suppressed extracellular signal-regulated kinase (ERK), p38 and c- Jun NH(2)-terminal kinase (JNK) mitogen-activated protein kinases (MAPKs) phosphorylation. Furthermore, we found SO suppresses the production of NO and IL-6 in LPS-stimulated peritoneal macrophage cells. High performance liquid chromatography (HPLC) analysis showed SO contains many active anti-inflammatory constituents such as liquiritigenin, baicalin, baicalein, glycyrrhizin and wogonin. We first elucidated the inhibitory mechanism of SO on inflammation induced by LPS in macrophage cells. Our results suggest SO has potential to be developed as a therapeutic agent for various inflammatory diseases.

Authors+Show Affiliations

Korean Medicine (KM)-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine, 461-24, Jeonmin-dong, Yuseong, Daejeon 305-811, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23246826

Citation

Oh, You-Chang, et al. "Anti-inflammatory Effect of Sosihotang Via Inhibition of Nuclear factor-κB and Mitogen-activated Protein Kinases Signaling Pathways in Lipopolysaccharide-stimulated RAW 264.7 Macrophage Cells." Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, vol. 53, 2013, pp. 343-51.
Oh YC, Cho WK, Jeong YH, et al. Anti-inflammatory effect of Sosihotang via inhibition of nuclear factor-κB and mitogen-activated protein kinases signaling pathways in lipopolysaccharide-stimulated RAW 264.7 macrophage cells. Food Chem Toxicol. 2013;53:343-51.
Oh, Y. C., Cho, W. K., Jeong, Y. H., Im, G. Y., Lee, K. J., Yang, H. J., & Ma, J. Y. (2013). Anti-inflammatory effect of Sosihotang via inhibition of nuclear factor-κB and mitogen-activated protein kinases signaling pathways in lipopolysaccharide-stimulated RAW 264.7 macrophage cells. Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, 53, pp. 343-51. doi:10.1016/j.fct.2012.12.006.
Oh YC, et al. Anti-inflammatory Effect of Sosihotang Via Inhibition of Nuclear factor-κB and Mitogen-activated Protein Kinases Signaling Pathways in Lipopolysaccharide-stimulated RAW 264.7 Macrophage Cells. Food Chem Toxicol. 2013;53:343-51. PubMed PMID: 23246826.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-inflammatory effect of Sosihotang via inhibition of nuclear factor-κB and mitogen-activated protein kinases signaling pathways in lipopolysaccharide-stimulated RAW 264.7 macrophage cells. AU - Oh,You-Chang, AU - Cho,Won-Kyung, AU - Jeong,Yun Hee, AU - Im,Ga Young, AU - Lee,Kwang Jin, AU - Yang,Hye Jin, AU - Ma,Jin Yeul, Y1 - 2012/12/12/ PY - 2012/11/19/received PY - 2012/12/04/accepted PY - 2012/12/19/entrez PY - 2012/12/19/pubmed PY - 2013/8/27/medline SP - 343 EP - 51 JF - Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association JO - Food Chem. Toxicol. VL - 53 N2 - Sosihotang (SO) is an herbal medication, which has been widely used to treat fever, chill and vomiting due to common cold in east-Asian countries. In this study, to provide insight into the effects of SO on inflammation, we investigated its effect on pro-inflammatory mediator production in RAW 264.7 cells and mouse peritoneal macrophages using lipopolysaccharide (LPS) stimulation. SO significantly inhibited the production of nitric oxide (NO), tumor necrosis factor (TNF)-α and interleukin (IL)-6 as well as gene expression of inducible nitric oxide synthase (iNOS), its synthesizing enzyme. In addition, SO inhibited nuclear factor (NF)-κB activation and suppressed extracellular signal-regulated kinase (ERK), p38 and c- Jun NH(2)-terminal kinase (JNK) mitogen-activated protein kinases (MAPKs) phosphorylation. Furthermore, we found SO suppresses the production of NO and IL-6 in LPS-stimulated peritoneal macrophage cells. High performance liquid chromatography (HPLC) analysis showed SO contains many active anti-inflammatory constituents such as liquiritigenin, baicalin, baicalein, glycyrrhizin and wogonin. We first elucidated the inhibitory mechanism of SO on inflammation induced by LPS in macrophage cells. Our results suggest SO has potential to be developed as a therapeutic agent for various inflammatory diseases. SN - 1873-6351 UR - https://www.unboundmedicine.com/medline/citation/23246826/Anti_inflammatory_effect_of_Sosihotang_via_inhibition_of_nuclear_factor_κB_and_mitogen_activated_protein_kinases_signaling_pathways_in_lipopolysaccharide_stimulated_RAW_264_7_macrophage_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0278-6915(12)00868-X DB - PRIME DP - Unbound Medicine ER -