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[Study on HPLC fingerprint chromatograms of cell wall-broken decoction pieces of Notoginseng].
Zhong Yao Cai. 2012 Jul; 35(7):1056-61.ZY

Abstract

OBJECTIVE

To establish the HPLC fingerprint chromatograms of crude Notoginseng, cell wall-broken powder and cell wall-broken decoction pieces of Notoginseng and provide evidence for quality control of cell wall-broken decoction pieces of Notoginseng.

METHODS

The HPLC procedure was performed on the chromatographic column of Hypersil ODS2, and the mobile phase was acetonitrile and water in gradient elution with the flow velocity of 1.0 mL/min. The detection wavelength was 203 nm and the column temperature was 25 degrees C. The chromatograms was analyzed with the software of "similarity evaluation system for chromatographic fingerprint of TCM".

RESULTS

Eight common peaks were pinpointed from the chromatograms of different batches of crude Notoginseng, cell-broken powder and cell-broken decoction pieces, the similarities of the chromatograms were all larger than 0.9.

CONCLUSION

The method of the HPLC fingerprint chromatogram is of good precision, reproducibility and stability,which is suitable for quality control of cell wall-broken decoction pieces of Notoginseng.

Authors+Show Affiliations

Chen WX
Piwei Institute, Guangzhou University of Chinese Medicine, Guangzhou 510405, China. chenweixuan@gmail.com
Liu M
No affiliation info available
Yan P
No affiliation info available
Zhan RT
No affiliation info available
Chen WW
No affiliation info available
Cheng JL
No affiliation info available

MeSH

Chromatography, High Pressure LiquidDrugs, Chinese HerbalPanax notoginsengPlant RootsPowdersQuality ControlReproducibility of ResultsRhizomeSaponins

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

23252266

Citation

Chen, Wei-Xuan, et al. "[Study On HPLC Fingerprint Chromatograms of Cell Wall-broken Decoction Pieces of Notoginseng]." Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials, vol. 35, no. 7, 2012, pp. 1056-61.
Chen WX, Liu M, Yan P, et al. [Study on HPLC fingerprint chromatograms of cell wall-broken decoction pieces of Notoginseng]. Zhong Yao Cai. 2012;35(7):1056-61.
Chen, W. X., Liu, M., Yan, P., Zhan, R. T., Chen, W. W., & Cheng, J. L. (2012). [Study on HPLC fingerprint chromatograms of cell wall-broken decoction pieces of Notoginseng]. Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials, 35(7), 1056-61.
Chen WX, et al. [Study On HPLC Fingerprint Chromatograms of Cell Wall-broken Decoction Pieces of Notoginseng]. Zhong Yao Cai. 2012;35(7):1056-61. PubMed PMID: 23252266.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Study on HPLC fingerprint chromatograms of cell wall-broken decoction pieces of Notoginseng]. AU - Chen,Wei-Xuan, AU - Liu,Min, AU - Yan,Ping, AU - Zhan,Ruo-Ting, AU - Chen,Wei-Wen, AU - Cheng,Jin-Le, PY - 2012/12/21/entrez PY - 2012/12/21/pubmed PY - 2013/3/13/medline SP - 1056 EP - 61 JF - Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials JO - Zhong Yao Cai VL - 35 IS - 7 N2 - OBJECTIVE: To establish the HPLC fingerprint chromatograms of crude Notoginseng, cell wall-broken powder and cell wall-broken decoction pieces of Notoginseng and provide evidence for quality control of cell wall-broken decoction pieces of Notoginseng. METHODS: The HPLC procedure was performed on the chromatographic column of Hypersil ODS2, and the mobile phase was acetonitrile and water in gradient elution with the flow velocity of 1.0 mL/min. The detection wavelength was 203 nm and the column temperature was 25 degrees C. The chromatograms was analyzed with the software of "similarity evaluation system for chromatographic fingerprint of TCM". RESULTS: Eight common peaks were pinpointed from the chromatograms of different batches of crude Notoginseng, cell-broken powder and cell-broken decoction pieces, the similarities of the chromatograms were all larger than 0.9. CONCLUSION: The method of the HPLC fingerprint chromatogram is of good precision, reproducibility and stability,which is suitable for quality control of cell wall-broken decoction pieces of Notoginseng. SN - 1001-4454 UR - https://www.unboundmedicine.com/medline/citation/23252266/[Study_on_HPLC_fingerprint_chromatograms_of_cell_wall_broken_decoction_pieces_of_Notoginseng]_ DB - PRIME DP - Unbound Medicine ER -