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Analysis of bumetanide in human urine by high-performance liquid chromatography with fluorescence detection and gas chromatography/mass spectrometry.
J Anal Toxicol. 1990 Mar-Apr; 14(2):123-6.JA

Abstract

Bumetanide is a potent diuretic. Its concentration in urine after therapeutic doses is in the low ng/mL range. A procedure using reversed-phase HPLC with fluorescence detection is described. Using this procedure, the authors have been able to measure bumetanide in a urine sample at 10 ng/mL with a signal-to-noise ratio of 7. Therefore, the detection limit is lower than this concentration. Confirmation is by a GC/MS procedure after methylating the diuretic. Derivatization is by extractive alkylation with tetrabutylammonium hydrogen sulfate as the phase transfer reagent and iodomethane as the methylating agent.

Authors+Show Affiliations

Department of Laboratory Medicine, Foothills Hospital, Calgary, Alberta, Canada.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

2325378

Citation

Gradeen, C Y., et al. "Analysis of Bumetanide in Human Urine By High-performance Liquid Chromatography With Fluorescence Detection and Gas Chromatography/mass Spectrometry." Journal of Analytical Toxicology, vol. 14, no. 2, 1990, pp. 123-6.
Gradeen CY, Billay DM, Chan SC. Analysis of bumetanide in human urine by high-performance liquid chromatography with fluorescence detection and gas chromatography/mass spectrometry. J Anal Toxicol. 1990;14(2):123-6.
Gradeen, C. Y., Billay, D. M., & Chan, S. C. (1990). Analysis of bumetanide in human urine by high-performance liquid chromatography with fluorescence detection and gas chromatography/mass spectrometry. Journal of Analytical Toxicology, 14(2), 123-6.
Gradeen CY, Billay DM, Chan SC. Analysis of Bumetanide in Human Urine By High-performance Liquid Chromatography With Fluorescence Detection and Gas Chromatography/mass Spectrometry. J Anal Toxicol. 1990 Mar-Apr;14(2):123-6. PubMed PMID: 2325378.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of bumetanide in human urine by high-performance liquid chromatography with fluorescence detection and gas chromatography/mass spectrometry. AU - Gradeen,C Y, AU - Billay,D M, AU - Chan,S C, PY - 1990/3/1/pubmed PY - 1990/3/1/medline PY - 1990/3/1/entrez SP - 123 EP - 6 JF - Journal of analytical toxicology JO - J Anal Toxicol VL - 14 IS - 2 N2 - Bumetanide is a potent diuretic. Its concentration in urine after therapeutic doses is in the low ng/mL range. A procedure using reversed-phase HPLC with fluorescence detection is described. Using this procedure, the authors have been able to measure bumetanide in a urine sample at 10 ng/mL with a signal-to-noise ratio of 7. Therefore, the detection limit is lower than this concentration. Confirmation is by a GC/MS procedure after methylating the diuretic. Derivatization is by extractive alkylation with tetrabutylammonium hydrogen sulfate as the phase transfer reagent and iodomethane as the methylating agent. SN - 0146-4760 UR - https://www.unboundmedicine.com/medline/citation/2325378/Analysis_of_bumetanide_in_human_urine_by_high_performance_liquid_chromatography_with_fluorescence_detection_and_gas_chromatography/mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -