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[Effects of expression of mitochondria long-chain fatty acid oxidative enzyme with different chain lengths of free fatty acids in trophoblast cells].
Zhonghua Yi Xue Za Zhi. 2012 Aug 07; 92(29):2034-7.ZY

Abstract

OBJECTIVE

To explore the interacting mechanisms and influences of different chain lengths of fatty acids and the expression of mitochondria long-chain 3 hydroxyacyl CoA dehydrogenase (LCHAD) in trophoblast cells.

METHODS

The serum-free trophoblast cells cultured in vitro were divided into 5 groups to receive the stimulations of DMEM/F12 medium without FFA (F-FFA), short-chain fatty acids (SC-FFA), medium-chain fatty acids (MC-FFA), long-chain fatty acids (LC-FFA), very long-chain fatty acids (VLC-FFA). The expressions of mRNA and protein of LCHAD in trophoblast cells were detected by real-time polymerase chain reaction (PCR) and Western blot.

RESULTS

Compared with the F-FFA, SC-FFA and MC-FFA groups, the expressions of gene and protein of LCHAD significantly decreased (P < 0.05) in the LC-FFA group. The expression of gene of LCHAD increased significantly in the VLC-FFA group (P < 0.05). But no difference existed in protein expression between the VLC-FFA group and other three groups (P > 0.05). Gene expression of LCHAD had no difference among the F-FFA, SC-FFA, MC-FFA groups (P > 0.05). Compared with the LC-FFA group, the expression of gene of LCHAD increased significantly in the VLC-FFA group (P < 0.05).

CONCLUSION

Free fatty acids may affect the expression of mitochondrial β-oxidation enzyme of LCHAD in trophoblast cells. Long-chain fatty acid alters the LCHAD gene protein expression. The correlation between very long chain fatty acids and the gene expression of LCHAD has been detected and their interactions needs further explorations. Short or medium chain fatty acids have no significant effect on the mitochondrial metabolism of fatty acid β-oxidation in trophoblast cells.

Authors+Show Affiliations

Department of Obstetrics & Gynecology, Peking University Third Hospital, Beijing 100191, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

chi

PubMed ID

23253803

Citation

Sun, Xiao-le, et al. "[Effects of Expression of Mitochondria Long-chain Fatty Acid Oxidative Enzyme With Different Chain Lengths of Free Fatty Acids in Trophoblast Cells]." Zhonghua Yi Xue Za Zhi, vol. 92, no. 29, 2012, pp. 2034-7.
Sun XL, Yang Z, Wang XY, et al. [Effects of expression of mitochondria long-chain fatty acid oxidative enzyme with different chain lengths of free fatty acids in trophoblast cells]. Zhonghua Yi Xue Za Zhi. 2012;92(29):2034-7.
Sun, X. L., Yang, Z., Wang, X. Y., Wang, J. L., & Wu, S. Y. (2012). [Effects of expression of mitochondria long-chain fatty acid oxidative enzyme with different chain lengths of free fatty acids in trophoblast cells]. Zhonghua Yi Xue Za Zhi, 92(29), 2034-7.
Sun XL, et al. [Effects of Expression of Mitochondria Long-chain Fatty Acid Oxidative Enzyme With Different Chain Lengths of Free Fatty Acids in Trophoblast Cells]. Zhonghua Yi Xue Za Zhi. 2012 Aug 7;92(29):2034-7. PubMed PMID: 23253803.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of expression of mitochondria long-chain fatty acid oxidative enzyme with different chain lengths of free fatty acids in trophoblast cells]. AU - Sun,Xiao-le, AU - Yang,Zi, AU - Wang,Xiao-ye, AU - Wang,Jia-lue, AU - Wu,Shu-ying, PY - 2012/12/21/entrez PY - 2012/12/21/pubmed PY - 2013/9/6/medline SP - 2034 EP - 7 JF - Zhonghua yi xue za zhi JO - Zhonghua Yi Xue Za Zhi VL - 92 IS - 29 N2 - OBJECTIVE: To explore the interacting mechanisms and influences of different chain lengths of fatty acids and the expression of mitochondria long-chain 3 hydroxyacyl CoA dehydrogenase (LCHAD) in trophoblast cells. METHODS: The serum-free trophoblast cells cultured in vitro were divided into 5 groups to receive the stimulations of DMEM/F12 medium without FFA (F-FFA), short-chain fatty acids (SC-FFA), medium-chain fatty acids (MC-FFA), long-chain fatty acids (LC-FFA), very long-chain fatty acids (VLC-FFA). The expressions of mRNA and protein of LCHAD in trophoblast cells were detected by real-time polymerase chain reaction (PCR) and Western blot. RESULTS: Compared with the F-FFA, SC-FFA and MC-FFA groups, the expressions of gene and protein of LCHAD significantly decreased (P < 0.05) in the LC-FFA group. The expression of gene of LCHAD increased significantly in the VLC-FFA group (P < 0.05). But no difference existed in protein expression between the VLC-FFA group and other three groups (P > 0.05). Gene expression of LCHAD had no difference among the F-FFA, SC-FFA, MC-FFA groups (P > 0.05). Compared with the LC-FFA group, the expression of gene of LCHAD increased significantly in the VLC-FFA group (P < 0.05). CONCLUSION: Free fatty acids may affect the expression of mitochondrial β-oxidation enzyme of LCHAD in trophoblast cells. Long-chain fatty acid alters the LCHAD gene protein expression. The correlation between very long chain fatty acids and the gene expression of LCHAD has been detected and their interactions needs further explorations. Short or medium chain fatty acids have no significant effect on the mitochondrial metabolism of fatty acid β-oxidation in trophoblast cells. SN - 0376-2491 UR - https://www.unboundmedicine.com/medline/citation/23253803/[Effects_of_expression_of_mitochondria_long_chain_fatty_acid_oxidative_enzyme_with_different_chain_lengths_of_free_fatty_acids_in_trophoblast_cells]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&amp;issn=0376-2491&amp;year=2012&amp;vol=92&amp;issue=29&amp;fpage=2034 DB - PRIME DP - Unbound Medicine ER -