Tags

Type your tag names separated by a space and hit enter

Systematic analysis of diuretic doping agents by HPLC screening and GC/MS confirmation.
J Anal Toxicol. 1990 Mar-Apr; 14(2):84-90.JA

Abstract

The simultaneous analysis of diuretic agents by reversed-phase liquid chromatography with a diode-array detector (DAD) was performed by using a gradient elution with acetonitrile and phosphate buffer on a Hypersil-ODS column. For the spiked urine the extraction recovery of solid-phase extraction (SPE) using Sep-Pak C18 cartridge was compared with that of liquid-liquid extraction (LLE) with diethyl ether at various pH. The standard calibration curves were linear from 0.20-20.0 micrograms/mL for all diuretic agents except amiloride, 1.0-20.0 micrograms/mL, and the detection limit was about 0.2 micrograms/mL for 3 mL of urine, except that of amiloride, which was 1.0 micrograms/mL. The confirmation analysis was performed by gas chromatography/mass spectrometry (GC/MS) following methylation. The characteristic mass fragment ions obtained by electron-impact (EI) ionization (70 eV) provided identification of each diuretic agent.

Authors+Show Affiliations

Doping Control Center, Korea Institute of Science and Technology, Cheongryang, Seoul.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2325382

Citation

Park, S J., et al. "Systematic Analysis of Diuretic Doping Agents By HPLC Screening and GC/MS Confirmation." Journal of Analytical Toxicology, vol. 14, no. 2, 1990, pp. 84-90.
Park SJ, Pyo HS, Kim YJ, et al. Systematic analysis of diuretic doping agents by HPLC screening and GC/MS confirmation. J Anal Toxicol. 1990;14(2):84-90.
Park, S. J., Pyo, H. S., Kim, Y. J., Kim, M. S., & Park, J. (1990). Systematic analysis of diuretic doping agents by HPLC screening and GC/MS confirmation. Journal of Analytical Toxicology, 14(2), 84-90.
Park SJ, et al. Systematic Analysis of Diuretic Doping Agents By HPLC Screening and GC/MS Confirmation. J Anal Toxicol. 1990 Mar-Apr;14(2):84-90. PubMed PMID: 2325382.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Systematic analysis of diuretic doping agents by HPLC screening and GC/MS confirmation. AU - Park,S J, AU - Pyo,H S, AU - Kim,Y J, AU - Kim,M S, AU - Park,J, PY - 1990/3/1/pubmed PY - 1990/3/1/medline PY - 1990/3/1/entrez SP - 84 EP - 90 JF - Journal of analytical toxicology JO - J Anal Toxicol VL - 14 IS - 2 N2 - The simultaneous analysis of diuretic agents by reversed-phase liquid chromatography with a diode-array detector (DAD) was performed by using a gradient elution with acetonitrile and phosphate buffer on a Hypersil-ODS column. For the spiked urine the extraction recovery of solid-phase extraction (SPE) using Sep-Pak C18 cartridge was compared with that of liquid-liquid extraction (LLE) with diethyl ether at various pH. The standard calibration curves were linear from 0.20-20.0 micrograms/mL for all diuretic agents except amiloride, 1.0-20.0 micrograms/mL, and the detection limit was about 0.2 micrograms/mL for 3 mL of urine, except that of amiloride, which was 1.0 micrograms/mL. The confirmation analysis was performed by gas chromatography/mass spectrometry (GC/MS) following methylation. The characteristic mass fragment ions obtained by electron-impact (EI) ionization (70 eV) provided identification of each diuretic agent. SN - 0146-4760 UR - https://www.unboundmedicine.com/medline/citation/2325382/Systematic_analysis_of_diuretic_doping_agents_by_HPLC_screening_and_GC/MS_confirmation_ L2 - https://academic.oup.com/jat/article-lookup/doi/10.1093/jat/14.2.84 DB - PRIME DP - Unbound Medicine ER -