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Postprandial plasma vitamin A metabolism in humans: a reassessment of the use of plasma retinyl esters as markers for intestinally derived chylomicrons and their remnants.
Metabolism. 1990 Apr; 39(4):357-65.M

Abstract

We investigated postprandial vitamin A metabolism by measuring retinyl ester, triglyceride, and apolipoprotein (apo)B-48 in the plasma lipoproteins of human subjects before and after fat-feeding. Following a 14-hour fast, eight healthy subjects (two men, six women, 28 to 79 years) were given a fat-rich meal (1 g fat/kg body weight) containing vitamin A (40 retinol equivalents per kilogram body weight). Blood was collected every 3 hours for 12 hours and lipoproteins were isolated by sequential ultracentrifugation. Mean plasma retinyl ester concentration peaked 6 hours after the fat-rich meal, whereas mean plasma triglyceride peaked at 3 hours. Data obtained from hourly samples in 3 subjects showed that changes in the postprandial plasma concentration of retinyl ester occurred 1 to 2 hours after changes in the plasma triglyceride concentration. In triglyceride-rich lipoproteins (TRL) of d less than 1.006 g/mL, retinyl ester similarly peaked at 6 hours, whereas triglyceride as well as apoB-48 peaked at 3 hours. Although retinyl esters were found mainly in TRL in the initial postprandial period (84%, 3 hours; 83%, 6 hours), in fasting and postprandial plasma, particularly 9 or more hours after fat-feeding, a large percentage of plasma retinyl esters were in low-density lipoproteins (LDL) (44%, fasting; 9%, 3 hours; 9%, 6 hours; 19%, 9 hours; 32%, 12 hours). A small percentage of retinyl esters were also found in postprandial high-density lipoproteins (HDL) (2% to 7%). ApoB-48 was not detected in LDL of fasting or postprandial plasma.(

ABSTRACT

TRUNCATED AT 250 WORDS)

Authors+Show Affiliations

US Department of Agriculture Human Nutrition Research Center on Aging, Tufts University, Boston, MA 02111.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2325560

Citation

Krasinski, S D., et al. "Postprandial Plasma Vitamin a Metabolism in Humans: a Reassessment of the Use of Plasma Retinyl Esters as Markers for Intestinally Derived Chylomicrons and Their Remnants." Metabolism: Clinical and Experimental, vol. 39, no. 4, 1990, pp. 357-65.
Krasinski SD, Cohn JS, Russell RM, et al. Postprandial plasma vitamin A metabolism in humans: a reassessment of the use of plasma retinyl esters as markers for intestinally derived chylomicrons and their remnants. Metab Clin Exp. 1990;39(4):357-65.
Krasinski, S. D., Cohn, J. S., Russell, R. M., & Schaefer, E. J. (1990). Postprandial plasma vitamin A metabolism in humans: a reassessment of the use of plasma retinyl esters as markers for intestinally derived chylomicrons and their remnants. Metabolism: Clinical and Experimental, 39(4), 357-65.
Krasinski SD, et al. Postprandial Plasma Vitamin a Metabolism in Humans: a Reassessment of the Use of Plasma Retinyl Esters as Markers for Intestinally Derived Chylomicrons and Their Remnants. Metab Clin Exp. 1990;39(4):357-65. PubMed PMID: 2325560.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Postprandial plasma vitamin A metabolism in humans: a reassessment of the use of plasma retinyl esters as markers for intestinally derived chylomicrons and their remnants. AU - Krasinski,S D, AU - Cohn,J S, AU - Russell,R M, AU - Schaefer,E J, PY - 1990/4/1/pubmed PY - 1990/4/1/medline PY - 1990/4/1/entrez SP - 357 EP - 65 JF - Metabolism: clinical and experimental JO - Metab. Clin. Exp. VL - 39 IS - 4 N2 - We investigated postprandial vitamin A metabolism by measuring retinyl ester, triglyceride, and apolipoprotein (apo)B-48 in the plasma lipoproteins of human subjects before and after fat-feeding. Following a 14-hour fast, eight healthy subjects (two men, six women, 28 to 79 years) were given a fat-rich meal (1 g fat/kg body weight) containing vitamin A (40 retinol equivalents per kilogram body weight). Blood was collected every 3 hours for 12 hours and lipoproteins were isolated by sequential ultracentrifugation. Mean plasma retinyl ester concentration peaked 6 hours after the fat-rich meal, whereas mean plasma triglyceride peaked at 3 hours. Data obtained from hourly samples in 3 subjects showed that changes in the postprandial plasma concentration of retinyl ester occurred 1 to 2 hours after changes in the plasma triglyceride concentration. In triglyceride-rich lipoproteins (TRL) of d less than 1.006 g/mL, retinyl ester similarly peaked at 6 hours, whereas triglyceride as well as apoB-48 peaked at 3 hours. Although retinyl esters were found mainly in TRL in the initial postprandial period (84%, 3 hours; 83%, 6 hours), in fasting and postprandial plasma, particularly 9 or more hours after fat-feeding, a large percentage of plasma retinyl esters were in low-density lipoproteins (LDL) (44%, fasting; 9%, 3 hours; 9%, 6 hours; 19%, 9 hours; 32%, 12 hours). A small percentage of retinyl esters were also found in postprandial high-density lipoproteins (HDL) (2% to 7%). ApoB-48 was not detected in LDL of fasting or postprandial plasma.(ABSTRACT TRUNCATED AT 250 WORDS) SN - 0026-0495 UR - https://www.unboundmedicine.com/medline/citation/2325560/Postprandial_plasma_vitamin_A_metabolism_in_humans:_a_reassessment_of_the_use_of_plasma_retinyl_esters_as_markers_for_intestinally_derived_chylomicrons_and_their_remnants_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0026-0495(90)90249-C DB - PRIME DP - Unbound Medicine ER -