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Apoferritin protein nanoparticles dually labeled with aptamer and horseradish peroxidase as a sensing probe for thrombin detection.
Anal Chim Acta. 2013 Jan 08; 759:53-60.AC

Abstract

A novel and ultrasensitive sandwich-type electrochemical aptasensor has been developed for the detection of thrombin, based on dual signal-amplification using HRP and apoferritin. Core/shell Fe(3)O(4)/Au magnetic nanoparticles (AuMNPs) loading aptamer1 (Apt1) was used as recognition elements, and apoferritin dually labeled with Aptamer2 (Apt2) and HRP was used as a detection probe. Sandwich-type complex, Apt1/thrombin/Apt2-apoferritin NPs-HRP was formed by the affinity reactions between AuMNPs-Apt1, thrombin, and Apt2-apoferritin-HRP. The complex was anchored on a screen-printed carbon electrode (SPCE). Differential pulse voltammetry (DPV) was used to monitor the electrode response. The proposed aptasensor yielded a linear current response to thrombin concentrations over a broad range of 0.5-100 pM with a detection limit of 0.07 pM (S/N=3). The detection signal was amplified by using apoferritin and HRP. This nanoparticle-based aptasensor offers a new method for rapid, sensitive, selective, and inexpensive quantification of thrombin, and offers a promising potential in protein detection and disease diagnosis.

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Authors+Show Affiliations

Zhao J
Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
Liu M
No affiliation info available
Zhang Y
No affiliation info available
Li H
No affiliation info available
Lin Y
No affiliation info available
Yao S
No affiliation info available

MeSH

AnimalsApoferritinsAptamers, NucleotideBiosensing TechniquesElectrochemical TechniquesEquipment DesignHorseradish PeroxidaseHumansImmobilized ProteinsLimit of DetectionMagnetite NanoparticlesThrombin

Pub Type(s)

Evaluation Study
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

23260676

Citation

Zhao, Jie, et al. "Apoferritin Protein Nanoparticles Dually Labeled With Aptamer and Horseradish Peroxidase as a Sensing Probe for Thrombin Detection." Analytica Chimica Acta, vol. 759, 2013, pp. 53-60.
Zhao J, Liu M, Zhang Y, et al. Apoferritin protein nanoparticles dually labeled with aptamer and horseradish peroxidase as a sensing probe for thrombin detection. Anal Chim Acta. 2013;759:53-60.
Zhao, J., Liu, M., Zhang, Y., Li, H., Lin, Y., & Yao, S. (2013). Apoferritin protein nanoparticles dually labeled with aptamer and horseradish peroxidase as a sensing probe for thrombin detection. Analytica Chimica Acta, 759, 53-60. https://doi.org/10.1016/j.aca.2012.10.041
Zhao J, et al. Apoferritin Protein Nanoparticles Dually Labeled With Aptamer and Horseradish Peroxidase as a Sensing Probe for Thrombin Detection. Anal Chim Acta. 2013 Jan 8;759:53-60. PubMed PMID: 23260676.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Apoferritin protein nanoparticles dually labeled with aptamer and horseradish peroxidase as a sensing probe for thrombin detection. AU - Zhao,Jie, AU - Liu,Meiling, AU - Zhang,Youyu, AU - Li,Haitao, AU - Lin,Yuehe, AU - Yao,Shouzhuo, Y1 - 2012/11/17/ PY - 2012/08/25/received PY - 2012/10/17/revised PY - 2012/10/20/accepted PY - 2012/12/25/entrez PY - 2012/12/25/pubmed PY - 2013/5/29/medline SP - 53 EP - 60 JF - Analytica chimica acta JO - Anal Chim Acta VL - 759 N2 - A novel and ultrasensitive sandwich-type electrochemical aptasensor has been developed for the detection of thrombin, based on dual signal-amplification using HRP and apoferritin. Core/shell Fe(3)O(4)/Au magnetic nanoparticles (AuMNPs) loading aptamer1 (Apt1) was used as recognition elements, and apoferritin dually labeled with Aptamer2 (Apt2) and HRP was used as a detection probe. Sandwich-type complex, Apt1/thrombin/Apt2-apoferritin NPs-HRP was formed by the affinity reactions between AuMNPs-Apt1, thrombin, and Apt2-apoferritin-HRP. The complex was anchored on a screen-printed carbon electrode (SPCE). Differential pulse voltammetry (DPV) was used to monitor the electrode response. The proposed aptasensor yielded a linear current response to thrombin concentrations over a broad range of 0.5-100 pM with a detection limit of 0.07 pM (S/N=3). The detection signal was amplified by using apoferritin and HRP. This nanoparticle-based aptasensor offers a new method for rapid, sensitive, selective, and inexpensive quantification of thrombin, and offers a promising potential in protein detection and disease diagnosis. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/23260676/Apoferritin_protein_nanoparticles_dually_labeled_with_aptamer_and_horseradish_peroxidase_as_a_sensing_probe_for_thrombin_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2670(12)01563-2 DB - PRIME DP - Unbound Medicine ER -