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Monotropein isolated from the roots of Morinda officinalis ameliorates proinflammatory mediators in RAW 264.7 macrophages and dextran sulfate sodium (DSS)-induced colitis via NF-κB inactivation.
Food Chem Toxicol. 2013 Mar; 53:263-71.FC

Abstract

We previously demonstrated that monotropein isolated from the roots of Morinda officinalis (Rubiaceae) has anti-inflammatory effects in vivo. In the present study, we investigated the molecular mechanisms underlying the anti-inflammatory effects of monotropein in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages and dextran sulfate sodium (DSS)-induced colitis mouse model. Monotropein was found to inhibit the expressions of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), and interleukin-1β (IL-1β) mRNA in LPS-induced RAW 264.7 macrophages. Treatment with monotropein decreased the DNA binding activity of nuclear factor-κB (NF-κB). Consistent with these findings, monotropein also suppressed phosphorylation and degradation of inhibitory κB-α (IκB-α), and consequently the translocations of NF-κB. In the DSS-induced colitis model, monotropein reduced disease activity index (DAI), myeloperoxidase (MPO) activity, and inflammation-related protein expressions by suppressing NF-κB activation in colon mucosa. Taken together, these findings suggest that the anti-inflammatory effects of monotropein are mainly related to the inhibition of the expressions of inflammatory mediators via NF-κB inactivation, and support its possible therapeutic role in colitis.

Authors+Show Affiliations

Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung Hee University, Seoul, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23261679

Citation

Shin, Ji-Sun, et al. "Monotropein Isolated From the Roots of Morinda Officinalis Ameliorates Proinflammatory Mediators in RAW 264.7 Macrophages and Dextran Sulfate Sodium (DSS)-induced Colitis Via NF-κB Inactivation." Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, vol. 53, 2013, pp. 263-71.
Shin JS, Yun KJ, Chung KS, et al. Monotropein isolated from the roots of Morinda officinalis ameliorates proinflammatory mediators in RAW 264.7 macrophages and dextran sulfate sodium (DSS)-induced colitis via NF-κB inactivation. Food Chem Toxicol. 2013;53:263-71.
Shin, J. S., Yun, K. J., Chung, K. S., Seo, K. H., Park, H. J., Cho, Y. W., Baek, N. I., Jang, D., & Lee, K. T. (2013). Monotropein isolated from the roots of Morinda officinalis ameliorates proinflammatory mediators in RAW 264.7 macrophages and dextran sulfate sodium (DSS)-induced colitis via NF-κB inactivation. Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, 53, 263-71. https://doi.org/10.1016/j.fct.2012.12.013
Shin JS, et al. Monotropein Isolated From the Roots of Morinda Officinalis Ameliorates Proinflammatory Mediators in RAW 264.7 Macrophages and Dextran Sulfate Sodium (DSS)-induced Colitis Via NF-κB Inactivation. Food Chem Toxicol. 2013;53:263-71. PubMed PMID: 23261679.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Monotropein isolated from the roots of Morinda officinalis ameliorates proinflammatory mediators in RAW 264.7 macrophages and dextran sulfate sodium (DSS)-induced colitis via NF-κB inactivation. AU - Shin,Ji-Sun, AU - Yun,Kyung-Jin, AU - Chung,Kyung-Sook, AU - Seo,Kyeong-Hwa, AU - Park,Hee-Juhn, AU - Cho,Young-Wuk, AU - Baek,Nam-In, AU - Jang,Daesik, AU - Lee,Kyung-Tae, Y1 - 2012/12/20/ PY - 2012/09/14/received PY - 2012/11/14/revised PY - 2012/12/08/accepted PY - 2012/12/25/entrez PY - 2012/12/25/pubmed PY - 2013/8/27/medline SP - 263 EP - 71 JF - Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association JO - Food Chem. Toxicol. VL - 53 N2 - We previously demonstrated that monotropein isolated from the roots of Morinda officinalis (Rubiaceae) has anti-inflammatory effects in vivo. In the present study, we investigated the molecular mechanisms underlying the anti-inflammatory effects of monotropein in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages and dextran sulfate sodium (DSS)-induced colitis mouse model. Monotropein was found to inhibit the expressions of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), and interleukin-1β (IL-1β) mRNA in LPS-induced RAW 264.7 macrophages. Treatment with monotropein decreased the DNA binding activity of nuclear factor-κB (NF-κB). Consistent with these findings, monotropein also suppressed phosphorylation and degradation of inhibitory κB-α (IκB-α), and consequently the translocations of NF-κB. In the DSS-induced colitis model, monotropein reduced disease activity index (DAI), myeloperoxidase (MPO) activity, and inflammation-related protein expressions by suppressing NF-κB activation in colon mucosa. Taken together, these findings suggest that the anti-inflammatory effects of monotropein are mainly related to the inhibition of the expressions of inflammatory mediators via NF-κB inactivation, and support its possible therapeutic role in colitis. SN - 1873-6351 UR - https://www.unboundmedicine.com/medline/citation/23261679/Monotropein_isolated_from_the_roots_of_Morinda_officinalis_ameliorates_proinflammatory_mediators_in_RAW_264_7_macrophages_and_dextran_sulfate_sodium__DSS__induced_colitis_via_NF_κB_inactivation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0278-6915(12)00886-1 DB - PRIME DP - Unbound Medicine ER -