TY - JOUR T1 - Simultaneous determination of six hydrophilic components in rat plasma after oral administration of Jitai tablet by liquid chromatography-electrospray ionization-tandem mass spectrometry: application to a pharmacokinetic study. AU - Wang,Shu-Ping, AU - Liu,Lei, AU - Wang,Ling-Ling, AU - Jiang,Peng, AU - Xiang,Li, AU - Zhang,Wei-Dong, AU - Liu,Run-Hui, Y1 - 2012/11/29/ PY - 2012/05/03/received PY - 2012/10/27/revised PY - 2012/11/01/accepted PY - 2012/12/25/entrez PY - 2012/12/25/pubmed PY - 2013/6/1/medline SP - 75 EP - 84 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 912 N2 - A liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) method was developed and validated for the simultaneous determination of amygdalin (ADL), danshensu (DSS), ferulic acid (FA), hydroxysafflor yellow A (HSYA), salvianolic acid A (SAA) and salvianolic acid B (SAB) in rat plasma. Plasma samples were pretreated by protein precipitation with acetonitrile. LC separation was performed on a Zorbax Eclipse Plus C18 column (3.0mm×100mm I.D, 1.8μm) with gradient elution using a mobile phase consisting of acetonitrile-0.1% formic acid in water at a flow rate of 0.3mL/min. ESI-MS spectra was acquired in negative ion multiple reaction monitoring mode. The mass transition ion-pair was followed as m/z 456.0→323.1, m/z 197.3→178.8, m/z 193.0→133.9, m/z 611.1→325.2, m/z 493.0→295.0, and m/z 717.0→519.0 for ADL, DSS, FA, HSYA, SAA and SAB, respectively. All analytes showed good linearity over a wide concentration range (r>0.99). The lower limit of quantification was 7ng/mL, 2ng/mL, 4ng/mL, 1ng/mL, 2ng/mL, and 4ng/mL for ADL, DSS, FA, HSYA, SAA and SAB, respectively. The mean recovery of the analytes ranged from 86.29% to 93.16%. The intra- and inter-day precisions were in the range of 1.50-9.98% and the accuracies were between 91.17% and 99.46%. The validated method was successfully applied to a pharmacokinetic study of the six hydrophilic components in rat plasma after oral administration of Jitai tablet. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/23261824/Simultaneous_determination_of_six_hydrophilic_components_in_rat_plasma_after_oral_administration_of_Jitai_tablet_by_liquid_chromatography_electrospray_ionization_tandem_mass_spectrometry:_application_to_a_pharmacokinetic_study_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(12)00702-7 DB - PRIME DP - Unbound Medicine ER -