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The molecular structures of major ampullate silk proteins of the wasp spider, Argiope bruennichi: a second blueprint for synthesizing de novo silk.

Abstract

The dragline silk of orb-weaving spiders possesses extremely high tensile strength and elasticity. To date, full-length sequences of only two genes encoding major ampullate silk protein (MaSp) in Latrodectus hesperus have been determined. In order to further understand this gene family, we utilized in this study a variety of strategies to isolate full-length MaSp1 and MaSp2 cDNAs in the wasp spider Argiope bruennichi. A. bruennichi MaSp1 and MaSp2 are primarily composed of remarkably homogeneous ensemble repeats containing several complex motifs, and both have highly conserved C-termini and N-termini. Two novel amino acid motifs, GGF and SGR, were found in MaSp1 and MaSp2, respectively. Amino acid composition analysis of silk, luminal contents and predicted sequences indicates that MaSp1 and MaSp2 are two major components of major ampullate glands and that the ratio of MaSp1 to MaSp2 is approximately 3:2 in dragline silk. Furthermore, both the MaSp1:MaSp2 ratio and the conserved termini are closely linked with the production of high quality synthetic fibers. Our results make an important contribution to our understanding of major ampullate silk protein structure and provide a second blueprint for creating new composite silk which mimics natural spider dragline silk.

Authors+Show Affiliations

State Key Laboratory of Silkworm Genome Biology, Southwest University, ChongQing 400715, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23262065

Citation

Zhang, Yang, et al. "The Molecular Structures of Major Ampullate Silk Proteins of the Wasp Spider, Argiope Bruennichi: a Second Blueprint for Synthesizing De Novo Silk." Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology, vol. 164, no. 3, 2013, pp. 151-8.
Zhang Y, Zhao AC, Sima YH, et al. The molecular structures of major ampullate silk proteins of the wasp spider, Argiope bruennichi: a second blueprint for synthesizing de novo silk. Comp Biochem Physiol B, Biochem Mol Biol. 2013;164(3):151-8.
Zhang, Y., Zhao, A. C., Sima, Y. H., Lu, C., Xiang, Z. H., & Nakagaki, M. (2013). The molecular structures of major ampullate silk proteins of the wasp spider, Argiope bruennichi: a second blueprint for synthesizing de novo silk. Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology, 164(3), pp. 151-8. doi:10.1016/j.cbpb.2012.12.002.
Zhang Y, et al. The Molecular Structures of Major Ampullate Silk Proteins of the Wasp Spider, Argiope Bruennichi: a Second Blueprint for Synthesizing De Novo Silk. Comp Biochem Physiol B, Biochem Mol Biol. 2013;164(3):151-8. PubMed PMID: 23262065.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The molecular structures of major ampullate silk proteins of the wasp spider, Argiope bruennichi: a second blueprint for synthesizing de novo silk. AU - Zhang,Yang, AU - Zhao,Ai-Chun, AU - Sima,Yang-Hu, AU - Lu,Cheng, AU - Xiang,Zhong-Huai, AU - Nakagaki,Masao, Y1 - 2012/12/20/ PY - 2012/10/08/received PY - 2012/12/09/revised PY - 2012/12/10/accepted PY - 2012/12/25/entrez PY - 2012/12/25/pubmed PY - 2013/10/1/medline SP - 151 EP - 8 JF - Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology JO - Comp. Biochem. Physiol. B, Biochem. Mol. Biol. VL - 164 IS - 3 N2 - The dragline silk of orb-weaving spiders possesses extremely high tensile strength and elasticity. To date, full-length sequences of only two genes encoding major ampullate silk protein (MaSp) in Latrodectus hesperus have been determined. In order to further understand this gene family, we utilized in this study a variety of strategies to isolate full-length MaSp1 and MaSp2 cDNAs in the wasp spider Argiope bruennichi. A. bruennichi MaSp1 and MaSp2 are primarily composed of remarkably homogeneous ensemble repeats containing several complex motifs, and both have highly conserved C-termini and N-termini. Two novel amino acid motifs, GGF and SGR, were found in MaSp1 and MaSp2, respectively. Amino acid composition analysis of silk, luminal contents and predicted sequences indicates that MaSp1 and MaSp2 are two major components of major ampullate glands and that the ratio of MaSp1 to MaSp2 is approximately 3:2 in dragline silk. Furthermore, both the MaSp1:MaSp2 ratio and the conserved termini are closely linked with the production of high quality synthetic fibers. Our results make an important contribution to our understanding of major ampullate silk protein structure and provide a second blueprint for creating new composite silk which mimics natural spider dragline silk. SN - 1879-1107 UR - https://www.unboundmedicine.com/medline/citation/23262065/The_molecular_structures_of_major_ampullate_silk_proteins_of_the_wasp_spider_Argiope_bruennichi:_a_second_blueprint_for_synthesizing_de_novo_silk_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1096-4959(12)00191-1 DB - PRIME DP - Unbound Medicine ER -