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Identification of a canonical SCF(SLF) complex involved in S-RNase-based self-incompatibility of Pyrus (Rosaceae).
Plant Mol Biol. 2013 Feb; 81(3):245-57.PM

Abstract

S-RNase-based self-incompatibility (SI) is an intraspecific reproductive barrier to prevent self-fertilization found in many species of the Solanaceae, Plantaginaceae and Rosaceae. In this system, S-RNase and SLF/SFB (S-locus F-box) genes have been shown to control the pistil and pollen SI specificity, respectively. Recent studies have shown that the SLF functions as a substrate receptor of a SCF (Skp1/Cullin1/F-box)-type E3 ubiquitin ligase complex to target S-RNases in Solanaceae and Plantaginaceae, but its role in Rosaceae remains largely undefined. Here we report the identification of two pollen-specific SLF-interacting Skp1-like (SSK) proteins, PbSSK1 and PbSSK2, in Pyrus bretschneideri from the tribe Pyreae of Rosaceae. Both yeast two-hybrid and pull-down assays demonstrated that they could connect PbSLFs to PbCUL1 to form a putative canonical SCF(SLF) (SSK/CUL1/SLF) complex in Pyrus. Furthermore, pull-down assays showed that the SSK proteins could bind SLF and CUL1 in a cross-species manner between Pyrus and Petunia. Additionally, phylogenetic analysis revealed that the SSK-like proteins from Solanaceae, Plantaginaceae and Rosaceae form a monoclade group, hinting their shared evolutionary origin. Taken together, with the recent identification of a canonical SCF(SFB) complex in Prunus of the tribe Amygdaleae of Rosaceae, our results show that a conserved canonical SCF(SLF/SFB) complex is present in Solanaceae, Plantaginaceae and Rosaceae, implying that S-RNase-based self-incompatibility shares a similar molecular and biochemical mechanism.

Authors+Show Affiliations

State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences and National Center for Plant Gene Research, Beijing 100101, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23263858

Citation

Xu, Chi, et al. "Identification of a Canonical SCF(SLF) Complex Involved in S-RNase-based Self-incompatibility of Pyrus (Rosaceae)." Plant Molecular Biology, vol. 81, no. 3, 2013, pp. 245-57.
Xu C, Li M, Wu J, et al. Identification of a canonical SCF(SLF) complex involved in S-RNase-based self-incompatibility of Pyrus (Rosaceae). Plant Mol Biol. 2013;81(3):245-57.
Xu, C., Li, M., Wu, J., Guo, H., Li, Q., Zhang, Y., Chai, J., Li, T., & Xue, Y. (2013). Identification of a canonical SCF(SLF) complex involved in S-RNase-based self-incompatibility of Pyrus (Rosaceae). Plant Molecular Biology, 81(3), 245-57. https://doi.org/10.1007/s11103-012-9995-x
Xu C, et al. Identification of a Canonical SCF(SLF) Complex Involved in S-RNase-based Self-incompatibility of Pyrus (Rosaceae). Plant Mol Biol. 2013;81(3):245-57. PubMed PMID: 23263858.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of a canonical SCF(SLF) complex involved in S-RNase-based self-incompatibility of Pyrus (Rosaceae). AU - Xu,Chi, AU - Li,Maofu, AU - Wu,Junkai, AU - Guo,Han, AU - Li,Qun, AU - Zhang,Yu'e, AU - Chai,Jijie, AU - Li,Tianzhong, AU - Xue,Yongbiao, Y1 - 2012/12/20/ PY - 2012/07/31/received PY - 2012/12/05/accepted PY - 2012/12/25/entrez PY - 2012/12/25/pubmed PY - 2013/3/15/medline SP - 245 EP - 57 JF - Plant molecular biology JO - Plant Mol Biol VL - 81 IS - 3 N2 - S-RNase-based self-incompatibility (SI) is an intraspecific reproductive barrier to prevent self-fertilization found in many species of the Solanaceae, Plantaginaceae and Rosaceae. In this system, S-RNase and SLF/SFB (S-locus F-box) genes have been shown to control the pistil and pollen SI specificity, respectively. Recent studies have shown that the SLF functions as a substrate receptor of a SCF (Skp1/Cullin1/F-box)-type E3 ubiquitin ligase complex to target S-RNases in Solanaceae and Plantaginaceae, but its role in Rosaceae remains largely undefined. Here we report the identification of two pollen-specific SLF-interacting Skp1-like (SSK) proteins, PbSSK1 and PbSSK2, in Pyrus bretschneideri from the tribe Pyreae of Rosaceae. Both yeast two-hybrid and pull-down assays demonstrated that they could connect PbSLFs to PbCUL1 to form a putative canonical SCF(SLF) (SSK/CUL1/SLF) complex in Pyrus. Furthermore, pull-down assays showed that the SSK proteins could bind SLF and CUL1 in a cross-species manner between Pyrus and Petunia. Additionally, phylogenetic analysis revealed that the SSK-like proteins from Solanaceae, Plantaginaceae and Rosaceae form a monoclade group, hinting their shared evolutionary origin. Taken together, with the recent identification of a canonical SCF(SFB) complex in Prunus of the tribe Amygdaleae of Rosaceae, our results show that a conserved canonical SCF(SLF/SFB) complex is present in Solanaceae, Plantaginaceae and Rosaceae, implying that S-RNase-based self-incompatibility shares a similar molecular and biochemical mechanism. SN - 1573-5028 UR - https://www.unboundmedicine.com/medline/citation/23263858/Identification_of_a_canonical_SCF_SLF__complex_involved_in_S_RNase_based_self_incompatibility_of_Pyrus__Rosaceae__ L2 - https://doi.org/10.1007/s11103-012-9995-x DB - PRIME DP - Unbound Medicine ER -