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Transepithelial transport of 6-O-caffeoylsophorose across Caco-2 cell monolayers.
Food Chem. 2013 May 01; 138(1):101-6.FC

Abstract

The aim of this study was to clarify the transport behaviour and mechanism of caffeic acid analogue bearing a sugar-moiety, 6-O-caffeoylsophorose (CS), in Caco-2 cells. The absorption of CS was investigated by its transport across Caco-2 cell monolayers using a high-performance liquid chromatography-time-of-flight-mass spectrometry (LC-TOF-MS). The permeation of CS was concentration-dependent and reached the plateau at >6 mM. The apparent permeability (P(app)) of CS in the apical-to-basolateral direction was 5.4×10(-7) cm/s, while in the reversed direction the P(app) value was significantly reduced (1.9×10(-7) cm/s). CS transport was competitively inhibited by phloretin, an inhibitor of monocarboxylic acid transporter (MCT). Benzoic acid, an MCT substrate, also reduced CS transport. A less significant change of CS transport was observed across Caco-2 cell monolayers pretreated with quercetin, a suppressor of tight-junction. These findings strongly indicate that CS, a caffeic acid analogue bearing sophorose moiety, can be transported across Caco-2 cell monolayers via the MCT pathway.

Authors+Show Affiliations

Department of Bioscience and Biotechnology, Division of Bioresource and Bioenviromental Sciences, Faculty of Agriculture, Graduated School of Kyushu University, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23265462

Citation

Phuong, Hoang Lan, et al. "Transepithelial Transport of 6-O-caffeoylsophorose Across Caco-2 Cell Monolayers." Food Chemistry, vol. 138, no. 1, 2013, pp. 101-6.
Phuong HL, Qiu J, Kuwahara T, et al. Transepithelial transport of 6-O-caffeoylsophorose across Caco-2 cell monolayers. Food Chem. 2013;138(1):101-6.
Phuong, H. L., Qiu, J., Kuwahara, T., Fukui, K., Yoshiyama, K., Matsugano, K., Terahara, N., & Matsui, T. (2013). Transepithelial transport of 6-O-caffeoylsophorose across Caco-2 cell monolayers. Food Chemistry, 138(1), 101-6. https://doi.org/10.1016/j.foodchem.2012.10.137
Phuong HL, et al. Transepithelial Transport of 6-O-caffeoylsophorose Across Caco-2 Cell Monolayers. Food Chem. 2013 May 1;138(1):101-6. PubMed PMID: 23265462.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transepithelial transport of 6-O-caffeoylsophorose across Caco-2 cell monolayers. AU - Phuong,Hoang Lan, AU - Qiu,Ju, AU - Kuwahara,Takashi, AU - Fukui,Keiichi, AU - Yoshiyama,Kayo, AU - Matsugano,Kazusato, AU - Terahara,Norihiko, AU - Matsui,Toshiro, Y1 - 2012/11/12/ PY - 2012/07/21/received PY - 2012/09/19/revised PY - 2012/10/22/accepted PY - 2012/12/26/entrez PY - 2012/12/26/pubmed PY - 2013/6/19/medline SP - 101 EP - 6 JF - Food chemistry JO - Food Chem VL - 138 IS - 1 N2 - The aim of this study was to clarify the transport behaviour and mechanism of caffeic acid analogue bearing a sugar-moiety, 6-O-caffeoylsophorose (CS), in Caco-2 cells. The absorption of CS was investigated by its transport across Caco-2 cell monolayers using a high-performance liquid chromatography-time-of-flight-mass spectrometry (LC-TOF-MS). The permeation of CS was concentration-dependent and reached the plateau at >6 mM. The apparent permeability (P(app)) of CS in the apical-to-basolateral direction was 5.4×10(-7) cm/s, while in the reversed direction the P(app) value was significantly reduced (1.9×10(-7) cm/s). CS transport was competitively inhibited by phloretin, an inhibitor of monocarboxylic acid transporter (MCT). Benzoic acid, an MCT substrate, also reduced CS transport. A less significant change of CS transport was observed across Caco-2 cell monolayers pretreated with quercetin, a suppressor of tight-junction. These findings strongly indicate that CS, a caffeic acid analogue bearing sophorose moiety, can be transported across Caco-2 cell monolayers via the MCT pathway. SN - 1873-7072 UR - https://www.unboundmedicine.com/medline/citation/23265462/Transepithelial_transport_of_6_O_caffeoylsophorose_across_Caco_2_cell_monolayers_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0308-8146(12)01711-6 DB - PRIME DP - Unbound Medicine ER -