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Diagnostic usefulness of component-resolved diagnosis by skin prick tests and specific IgE to single allergen components in children with allergy to fruits and vegetables.
Allergol Immunopathol (Madr) 2014 Mar-Apr; 42(2):127-35AI

Abstract

INTRODUCTION

The accurate identification of sensitizing proteins in patients allergic to plant-derived foods is extremely important, allowing a correct dietary advice. We aimed to evaluate the diagnostic usefulness of skin prick tests (SPT) and specific IgE (sIgE) with single molecular allergen components in children with allergy to fruits and vegetables.

METHODS

Twenty children underwent SPT with a palm profilin (Pho d 2, 50 μg/mL); a Mal d 1-enriched apple extract (2 μg/mL) (PR-10 allergen); and a peach Lipid Transfer Protein (LTP) (Pru p 3, 30 μg/mL). Detection of sIgE to rBet v 1, rBet v 2, Phl p 12 and Pru p 3 was also measured.

RESULTS

Allergy to multiple fruits and vegetables was observed in 11 (55%) children. Sensitization by SPT to Pho d 2, Mal d 1, and Pru p 3 occurred in 5, 7, and 8 cases, respectively. LTP sensitization appeared to be associated with peach allergy but not with severe reactions, and profilins sensitization to melon and tomato allergy. Kiwi sensitization (12 cases), the plant-derived food that caused more allergic reactions, seemed mostly species-specific. The concordance of SPT extracts and sIgE to the corresponding pan-allergens was high for profilins (k=0.857) and LTP (k=0.706), while for PR-10 allergens it was absent (k=0.079).

CONCLUSIONS

Pan-allergen sensitization in children with allergy to fruits and vegetables was common and often multiple. There was no association of severe reactions to LTP sensitization. The introduction of routine SPT to pan-allergens can be a simple and feasible way of improving diagnostic and therapeutic efficacy.

Authors+Show Affiliations

Unidade de Imunoalergologia - Centro Hospitalar do Porto, E.P.E., Porto, Portugal. Electronic address: te_vieira@yahoo.com.br.Unidade de Imunoalergologia - Centro Hospitalar do Porto, E.P.E., Porto, Portugal.Serviço de Imunologia - Centro Hospitalar do Porto, E.P.E., Porto, Portugal.Unidade de Imunoalergologia - Centro Hospitalar do Porto, E.P.E., Porto, Portugal.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23266139

Citation

Vieira, T, et al. "Diagnostic Usefulness of Component-resolved Diagnosis By Skin Prick Tests and Specific IgE to Single Allergen Components in Children With Allergy to Fruits and Vegetables." Allergologia Et Immunopathologia, vol. 42, no. 2, 2014, pp. 127-35.
Vieira T, Cunha L, Neves E, et al. Diagnostic usefulness of component-resolved diagnosis by skin prick tests and specific IgE to single allergen components in children with allergy to fruits and vegetables. Allergol Immunopathol (Madr). 2014;42(2):127-35.
Vieira, T., Cunha, L., Neves, E., & Falcão, H. (2014). Diagnostic usefulness of component-resolved diagnosis by skin prick tests and specific IgE to single allergen components in children with allergy to fruits and vegetables. Allergologia Et Immunopathologia, 42(2), pp. 127-35. doi:10.1016/j.aller.2012.09.001.
Vieira T, et al. Diagnostic Usefulness of Component-resolved Diagnosis By Skin Prick Tests and Specific IgE to Single Allergen Components in Children With Allergy to Fruits and Vegetables. Allergol Immunopathol (Madr). 2014;42(2):127-35. PubMed PMID: 23266139.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diagnostic usefulness of component-resolved diagnosis by skin prick tests and specific IgE to single allergen components in children with allergy to fruits and vegetables. AU - Vieira,T, AU - Cunha,L, AU - Neves,E, AU - Falcão,H, Y1 - 2012/12/21/ PY - 2012/07/08/received PY - 2012/09/27/revised PY - 2012/09/28/accepted PY - 2012/12/26/entrez PY - 2012/12/26/pubmed PY - 2014/12/15/medline KW - Children KW - Component-resolved diagnosis KW - Food allergy KW - Fruits and vegetables KW - Pan-allergens SP - 127 EP - 35 JF - Allergologia et immunopathologia JO - Allergol Immunopathol (Madr) VL - 42 IS - 2 N2 - INTRODUCTION: The accurate identification of sensitizing proteins in patients allergic to plant-derived foods is extremely important, allowing a correct dietary advice. We aimed to evaluate the diagnostic usefulness of skin prick tests (SPT) and specific IgE (sIgE) with single molecular allergen components in children with allergy to fruits and vegetables. METHODS: Twenty children underwent SPT with a palm profilin (Pho d 2, 50 μg/mL); a Mal d 1-enriched apple extract (2 μg/mL) (PR-10 allergen); and a peach Lipid Transfer Protein (LTP) (Pru p 3, 30 μg/mL). Detection of sIgE to rBet v 1, rBet v 2, Phl p 12 and Pru p 3 was also measured. RESULTS: Allergy to multiple fruits and vegetables was observed in 11 (55%) children. Sensitization by SPT to Pho d 2, Mal d 1, and Pru p 3 occurred in 5, 7, and 8 cases, respectively. LTP sensitization appeared to be associated with peach allergy but not with severe reactions, and profilins sensitization to melon and tomato allergy. Kiwi sensitization (12 cases), the plant-derived food that caused more allergic reactions, seemed mostly species-specific. The concordance of SPT extracts and sIgE to the corresponding pan-allergens was high for profilins (k=0.857) and LTP (k=0.706), while for PR-10 allergens it was absent (k=0.079). CONCLUSIONS: Pan-allergen sensitization in children with allergy to fruits and vegetables was common and often multiple. There was no association of severe reactions to LTP sensitization. The introduction of routine SPT to pan-allergens can be a simple and feasible way of improving diagnostic and therapeutic efficacy. SN - 1578-1267 UR - https://www.unboundmedicine.com/medline/citation/23266139/Diagnostic_usefulness_of_component_resolved_diagnosis_by_skin_prick_tests_and_specific_IgE_to_single_allergen_components_in_children_with_allergy_to_fruits_and_vegetables_ L2 - http://www.elsevier.es/en/linksolver/ft/pii/S0301-0546(12)00275-3 DB - PRIME DP - Unbound Medicine ER -