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Optimizing human oocyte cryopreservation for fertility preservation patients: should we mature then freeze or freeze then mature?
Fertil Steril. 2013 Apr; 99(5):1356-62.FS

Abstract

OBJECTIVE

To evaluate the maturation and post-thaw survival rates of immature oocytes to determine whether in vitro maturation (IVM) should be attempted prior to or after cryopreservation.

DESIGN

Nonrandomized observational study.

SETTING

Private academic and clinical reproductive center.

PATIENT(S)

Patients (n = 71) who donated immature unusable oocytes after vaginal oocyte retrieval (VOR) after undergoing controlled ovarian hyperstimulation using a standard GnRH antagonist protocol.

INTERVENTION(S)

Germinal vesicle (GV), metaphase I (MI), and metaphase II (MII) oocytes (n = 175) were obtained from consenting IVF patients for fresh IVM, post-thaw IVM, or control group. In the fresh IVM group, GV- and MI- stage oocytes (n = 69) were cultured for 24 hours, matured in vitro (IVM-MII), cryopreserved, thawed, and evaluated for survival. In the post-thaw IVM group, GV- and MI- stage oocytes (n = 27) were frozen on day 0, thawed, evaluated for survival, and cultured for 24-hour IVM. MII donor oocytes (n = 79) were cryopreserved and thawed as a control.

MAIN OUTCOME MEASURE(S)

Survival postfreeze and oocyte development to the MII stage was analyzed using a χ(2) analysis.

RESULT(S)

Fresh IVM had a significantly higher maturation rate than post-thaw IVM.

CONCLUSION(S)

Oocyte cryopreservation is important for patients at risk of ovarian cancer, elective fertility preservation, and, potentially, for ovum donation. The superior maturation rate of GV and MI oocytes in the fresh versus post-thaw groups provides strong evidence for maturing oocytes to the MII stage before cryopreservation.

Authors+Show Affiliations

Reproductive Medicine Associates of New York, New York, NY 10022, USA. jlee@rmany.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23266213

Citation

Lee, Joseph A., et al. "Optimizing Human Oocyte Cryopreservation for Fertility Preservation Patients: Should We Mature Then Freeze or Freeze Then Mature?" Fertility and Sterility, vol. 99, no. 5, 2013, pp. 1356-62.
Lee JA, Barritt J, Moschini RM, et al. Optimizing human oocyte cryopreservation for fertility preservation patients: should we mature then freeze or freeze then mature? Fertil Steril. 2013;99(5):1356-62.
Lee, J. A., Barritt, J., Moschini, R. M., Slifkin, R. E., & Copperman, A. B. (2013). Optimizing human oocyte cryopreservation for fertility preservation patients: should we mature then freeze or freeze then mature? Fertility and Sterility, 99(5), 1356-62. https://doi.org/10.1016/j.fertnstert.2012.11.042
Lee JA, et al. Optimizing Human Oocyte Cryopreservation for Fertility Preservation Patients: Should We Mature Then Freeze or Freeze Then Mature. Fertil Steril. 2013;99(5):1356-62. PubMed PMID: 23266213.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Optimizing human oocyte cryopreservation for fertility preservation patients: should we mature then freeze or freeze then mature? AU - Lee,Joseph A, AU - Barritt,Jason, AU - Moschini,Rose Marie, AU - Slifkin,Richard E, AU - Copperman,Alan B, Y1 - 2012/12/21/ PY - 2012/07/29/received PY - 2012/11/09/revised PY - 2012/11/20/accepted PY - 2012/12/26/entrez PY - 2012/12/26/pubmed PY - 2013/7/3/medline SP - 1356 EP - 62 JF - Fertility and sterility JO - Fertil Steril VL - 99 IS - 5 N2 - OBJECTIVE: To evaluate the maturation and post-thaw survival rates of immature oocytes to determine whether in vitro maturation (IVM) should be attempted prior to or after cryopreservation. DESIGN: Nonrandomized observational study. SETTING: Private academic and clinical reproductive center. PATIENT(S): Patients (n = 71) who donated immature unusable oocytes after vaginal oocyte retrieval (VOR) after undergoing controlled ovarian hyperstimulation using a standard GnRH antagonist protocol. INTERVENTION(S): Germinal vesicle (GV), metaphase I (MI), and metaphase II (MII) oocytes (n = 175) were obtained from consenting IVF patients for fresh IVM, post-thaw IVM, or control group. In the fresh IVM group, GV- and MI- stage oocytes (n = 69) were cultured for 24 hours, matured in vitro (IVM-MII), cryopreserved, thawed, and evaluated for survival. In the post-thaw IVM group, GV- and MI- stage oocytes (n = 27) were frozen on day 0, thawed, evaluated for survival, and cultured for 24-hour IVM. MII donor oocytes (n = 79) were cryopreserved and thawed as a control. MAIN OUTCOME MEASURE(S): Survival postfreeze and oocyte development to the MII stage was analyzed using a χ(2) analysis. RESULT(S): Fresh IVM had a significantly higher maturation rate than post-thaw IVM. CONCLUSION(S): Oocyte cryopreservation is important for patients at risk of ovarian cancer, elective fertility preservation, and, potentially, for ovum donation. The superior maturation rate of GV and MI oocytes in the fresh versus post-thaw groups provides strong evidence for maturing oocytes to the MII stage before cryopreservation. SN - 1556-5653 UR - https://www.unboundmedicine.com/medline/citation/23266213/Optimizing_human_oocyte_cryopreservation_for_fertility_preservation_patients:_should_we_mature_then_freeze_or_freeze_then_mature L2 - https://linkinghub.elsevier.com/retrieve/pii/S0015-0282(12)02454-5 DB - PRIME DP - Unbound Medicine ER -