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ZmCPK11 is involved in abscisic acid-induced antioxidant defence and functions upstream of ZmMPK5 in abscisic acid signalling in maize.
J Exp Bot. 2013 Feb; 64(4):871-84.JE

Abstract

Calcium-dependent protein kinases (CDPKs) have been shown to be involved in abscisic acid (ABA)-mediated physiological processes, including seed germination, post-germination growth, stomatal movement, and plant stress tolerance. However, it is not clear whether CDPKs are involved in ABA-induced antioxidant defence. In the present study, the role of the maize CDPK ZmCPK11 in ABA-induced antioxidant defence and the relationship between ZmCPK11 and ZmMPK5, a maize ABA-activated mitogen-activated protein kinase (MAPK), in ABA signalling were investigated. Treatments with ABA and H(2)O(2) induced the expression of ZmCPK11 and increased the activity of ZmCPK11, while H(2)O(2) was required for the ABA-induced increases in the expression and the activity of ZmCPK11. The transient gene expression analysis and the transient RNA interference (RNAi) test in protoplasts showed that ZmCPK11 is involved in ABA-induced up-regulation of the expression and the activities of superoxide dismutase (SOD) and ascorbate peroxidase (APX), and in the production of H(2)O(2). Further, ZmCPK11 was shown to be required for the up-regulation of the expression and the activity of ZmMPK5 in ABA signalling, but ZmMPK5 had very little effect on the ABA-induced up-regulation of the expression and the activity of ZmCPK11. Moreover, the transient gene expression analysis in combination with the transient RNAi test in protoplasts showed that ZmCPK11 acts upstream of ZmMPK5 to regulate the activities of antioxidant enzymes. These results indicate that ZmCPK11 is involved in ABA-induced antioxidant defence and functions upstream of ZmMPK5 in ABA signalling in maize.

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Authors+Show Affiliations

Ding Y
College of Life Sciences, Nanjing Agricultural University, Nanjing, China.
Cao J
No affiliation info available
Ni L
No affiliation info available
Zhu Y
No affiliation info available
Zhang A
No affiliation info available
Tan M
No affiliation info available
Jiang M
No affiliation info available

MeSH

Abscisic AcidAntioxidantsAscorbate PeroxidasesEnzyme ActivationEnzyme AssaysGene Expression Regulation, EnzymologicGene Expression Regulation, PlantGenes, PlantHydrogen PeroxideMitogen-Activated Protein KinasesPlant LeavesPlant ProteinsProtoplastsRNA InterferenceRNA, PlantSignal TransductionSuperoxide DismutaseZea mays

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23268839

Citation

Ding, Yanfen, et al. "ZmCPK11 Is Involved in Abscisic Acid-induced Antioxidant Defence and Functions Upstream of ZmMPK5 in Abscisic Acid Signalling in Maize." Journal of Experimental Botany, vol. 64, no. 4, 2013, pp. 871-84.
Ding Y, Cao J, Ni L, et al. ZmCPK11 is involved in abscisic acid-induced antioxidant defence and functions upstream of ZmMPK5 in abscisic acid signalling in maize. J Exp Bot. 2013;64(4):871-84.
Ding, Y., Cao, J., Ni, L., Zhu, Y., Zhang, A., Tan, M., & Jiang, M. (2013). ZmCPK11 is involved in abscisic acid-induced antioxidant defence and functions upstream of ZmMPK5 in abscisic acid signalling in maize. Journal of Experimental Botany, 64(4), 871-84. https://doi.org/10.1093/jxb/ers366
Ding Y, et al. ZmCPK11 Is Involved in Abscisic Acid-induced Antioxidant Defence and Functions Upstream of ZmMPK5 in Abscisic Acid Signalling in Maize. J Exp Bot. 2013;64(4):871-84. PubMed PMID: 23268839.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - ZmCPK11 is involved in abscisic acid-induced antioxidant defence and functions upstream of ZmMPK5 in abscisic acid signalling in maize. AU - Ding,Yanfen, AU - Cao,Jianmei, AU - Ni,Lan, AU - Zhu,Yuan, AU - Zhang,Aying, AU - Tan,Mingpu, AU - Jiang,Mingyi, Y1 - 2012/12/26/ PY - 2012/12/28/entrez PY - 2012/12/28/pubmed PY - 2013/7/28/medline SP - 871 EP - 84 JF - Journal of experimental botany JO - J Exp Bot VL - 64 IS - 4 N2 - Calcium-dependent protein kinases (CDPKs) have been shown to be involved in abscisic acid (ABA)-mediated physiological processes, including seed germination, post-germination growth, stomatal movement, and plant stress tolerance. However, it is not clear whether CDPKs are involved in ABA-induced antioxidant defence. In the present study, the role of the maize CDPK ZmCPK11 in ABA-induced antioxidant defence and the relationship between ZmCPK11 and ZmMPK5, a maize ABA-activated mitogen-activated protein kinase (MAPK), in ABA signalling were investigated. Treatments with ABA and H(2)O(2) induced the expression of ZmCPK11 and increased the activity of ZmCPK11, while H(2)O(2) was required for the ABA-induced increases in the expression and the activity of ZmCPK11. The transient gene expression analysis and the transient RNA interference (RNAi) test in protoplasts showed that ZmCPK11 is involved in ABA-induced up-regulation of the expression and the activities of superoxide dismutase (SOD) and ascorbate peroxidase (APX), and in the production of H(2)O(2). Further, ZmCPK11 was shown to be required for the up-regulation of the expression and the activity of ZmMPK5 in ABA signalling, but ZmMPK5 had very little effect on the ABA-induced up-regulation of the expression and the activity of ZmCPK11. Moreover, the transient gene expression analysis in combination with the transient RNAi test in protoplasts showed that ZmCPK11 acts upstream of ZmMPK5 to regulate the activities of antioxidant enzymes. These results indicate that ZmCPK11 is involved in ABA-induced antioxidant defence and functions upstream of ZmMPK5 in ABA signalling in maize. SN - 1460-2431 UR - https://www.unboundmedicine.com/medline/citation/23268839/ZmCPK11_is_involved_in_abscisic_acid_induced_antioxidant_defence_and_functions_upstream_of_ZmMPK5_in_abscisic_acid_signalling_in_maize_ L2 - https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/ers366 DB - PRIME DP - Unbound Medicine ER -