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Susceptibility of Cry1Ab maize-resistant and -susceptible strains of sugarcane borer (Lepidoptera: Crambidae) to four individual Cry proteins.
J Invertebr Pathol. 2013 Mar; 112(3):267-72.JI

Abstract

Sugarcane borer, Diatraea saccharalis (F.), is a major target of Bt maize in South America and many areas of the US mid-south region. Six laboratory strains of D. saccharalis were established from six single-pair F(2) families possessing major resistance alleles to Cry1Ab maize hybrids. Susceptibility of the six strains was evaluated on diet treated with each of four purified trypsin-activated Bt proteins, Cry1Ab, Cry1Aa, Cry1Ac and Cry1F. Bt susceptibility of the six strains was compared with that of known Cry1Ab-susceptible and -resistant strains of D. saccharalis. At least two of the six strains demonstrated a similar level (>526-fold) of resistance to Cry1Ab as shown in the known Cry1Ab-resistant strain, while resistance levels were relatively lower for other strains (116- to 129-fold). All the six strains were highly cross-resistant to Cry1Aa (71- to 292-fold) and Cry1Ac (30- to 248-fold), but only with a low level to Cry1F (<7-fold). Larval growth of all six strains was also inhibited on Bt-treated diet, but, except for Cry1F, the growth inhibition of the six strains was considerably less than that of the Cry1Ab-susceptible larvae. The results provide clear evidence that the observed resistance to Cry1Ab maize in the six strains is a result of resistance to the Cry1Ab protein in the plants. The low level of cross-resistance between Cry1A and Cry1F suggests that pyramiding these two types of Bt proteins into a plant could be a good strategy for managing D. saccharalis.

Authors+Show Affiliations

Department of Entomology, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

23270874

Citation

Zhang, Liping, et al. "Susceptibility of Cry1Ab Maize-resistant and -susceptible Strains of Sugarcane Borer (Lepidoptera: Crambidae) to Four Individual Cry Proteins." Journal of Invertebrate Pathology, vol. 112, no. 3, 2013, pp. 267-72.
Zhang L, Huang F, Rogers Leonard B, et al. Susceptibility of Cry1Ab maize-resistant and -susceptible strains of sugarcane borer (Lepidoptera: Crambidae) to four individual Cry proteins. J Invertebr Pathol. 2013;112(3):267-72.
Zhang, L., Huang, F., Rogers Leonard, B., Chen, M., Clark, T., Zhu, Y. C., Wangila, D. S., Yang, F., & Niu, Y. (2013). Susceptibility of Cry1Ab maize-resistant and -susceptible strains of sugarcane borer (Lepidoptera: Crambidae) to four individual Cry proteins. Journal of Invertebrate Pathology, 112(3), 267-72. https://doi.org/10.1016/j.jip.2012.12.007
Zhang L, et al. Susceptibility of Cry1Ab Maize-resistant and -susceptible Strains of Sugarcane Borer (Lepidoptera: Crambidae) to Four Individual Cry Proteins. J Invertebr Pathol. 2013;112(3):267-72. PubMed PMID: 23270874.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Susceptibility of Cry1Ab maize-resistant and -susceptible strains of sugarcane borer (Lepidoptera: Crambidae) to four individual Cry proteins. AU - Zhang,Liping, AU - Huang,Fangneng, AU - Rogers Leonard,B, AU - Chen,Mao, AU - Clark,Thomas, AU - Zhu,Yu Cheng, AU - Wangila,David S, AU - Yang,Fei, AU - Niu,Ying, Y1 - 2012/12/24/ PY - 2012/08/29/received PY - 2012/12/03/revised PY - 2012/12/15/accepted PY - 2012/12/29/entrez PY - 2012/12/29/pubmed PY - 2013/11/20/medline SP - 267 EP - 72 JF - Journal of invertebrate pathology JO - J Invertebr Pathol VL - 112 IS - 3 N2 - Sugarcane borer, Diatraea saccharalis (F.), is a major target of Bt maize in South America and many areas of the US mid-south region. Six laboratory strains of D. saccharalis were established from six single-pair F(2) families possessing major resistance alleles to Cry1Ab maize hybrids. Susceptibility of the six strains was evaluated on diet treated with each of four purified trypsin-activated Bt proteins, Cry1Ab, Cry1Aa, Cry1Ac and Cry1F. Bt susceptibility of the six strains was compared with that of known Cry1Ab-susceptible and -resistant strains of D. saccharalis. At least two of the six strains demonstrated a similar level (>526-fold) of resistance to Cry1Ab as shown in the known Cry1Ab-resistant strain, while resistance levels were relatively lower for other strains (116- to 129-fold). All the six strains were highly cross-resistant to Cry1Aa (71- to 292-fold) and Cry1Ac (30- to 248-fold), but only with a low level to Cry1F (<7-fold). Larval growth of all six strains was also inhibited on Bt-treated diet, but, except for Cry1F, the growth inhibition of the six strains was considerably less than that of the Cry1Ab-susceptible larvae. The results provide clear evidence that the observed resistance to Cry1Ab maize in the six strains is a result of resistance to the Cry1Ab protein in the plants. The low level of cross-resistance between Cry1A and Cry1F suggests that pyramiding these two types of Bt proteins into a plant could be a good strategy for managing D. saccharalis. SN - 1096-0805 UR - https://www.unboundmedicine.com/medline/citation/23270874/Susceptibility_of_Cry1Ab_maize_resistant_and__susceptible_strains_of_sugarcane_borer__Lepidoptera:_Crambidae__to_four_individual_Cry_proteins_ DB - PRIME DP - Unbound Medicine ER -