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Human rhinoviruses including group C are common in stool samples of young Finnish children.
J Clin Virol. 2013 Mar; 56(3):250-4.JC

Abstract

BACKGROUND

Human rhinoviruses (HRVs) are common causes of viral respiratory infections. They have been widely studied in respiratory samples in hospital patient series but only a few studies have been performed to assess their occurrence in other sample types and their circulation in healthy children background population.

OBJECTIVES

To analyze the frequency of HRVs in the background population in Finland by screening HRV RNA from stool samples longitudinally collected in a cohort of young children.

STUDY DESIGN

Altogether 4184 stool samples were collected regularly from a cohort of children who were observed from birth. Samples were screened for the presence of RNA of HRVs using RT-PCR. HRV specific sequences were identified by sequencing the VP1 or VP4/VP2 coding region. Virus isolation was performed using four different cell lines and the result was confirmed by real time PCR.

RESULTS

A total of 9% of the stool samples were positive for HRV RNA. Sequence analysis indicated that the most prevalent species was HRV-A, and the most prevalent serotype was HRV61. HRV-B and HRV-C species were also detected. One of the six tested rhinovirus positive samples retained its infectivity and was able to grow in RD and GMK cells.

CONCLUSIONS

Our study shows that HRVs are frequently detected in the stool samples from the population of young children. We also show that HRV-C, which can cause severe illnesses in children, is commonly circulating in young children in Finland.

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Authors+Show Affiliations

Honkanen H
University of Tampere, School of Medicine, Tampere, Finland. hanna.honkanen@uta.fi
Oikarinen S
No affiliation info available
Peltonen P
No affiliation info available
Simell O
No affiliation info available
Ilonen J
No affiliation info available
Veijola R
No affiliation info available
Knip M
No affiliation info available
Hyöty H
No affiliation info available

MeSH

ChildChild, PreschoolFecesFemaleFinlandGenotypeHumansInfantMalePicornaviridae InfectionsPrevalenceProspective StudiesRNA, ViralReverse Transcriptase Polymerase Chain ReactionRhinovirusSequence Analysis, DNAViral Structural Proteins

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23273491

Citation

Honkanen, Hanna, et al. "Human Rhinoviruses Including Group C Are Common in Stool Samples of Young Finnish Children." Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, vol. 56, no. 3, 2013, pp. 250-4.
Honkanen H, Oikarinen S, Peltonen P, et al. Human rhinoviruses including group C are common in stool samples of young Finnish children. J Clin Virol. 2013;56(3):250-4.
Honkanen, H., Oikarinen, S., Peltonen, P., Simell, O., Ilonen, J., Veijola, R., Knip, M., & Hyöty, H. (2013). Human rhinoviruses including group C are common in stool samples of young Finnish children. Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, 56(3), 250-4. https://doi.org/10.1016/j.jcv.2012.11.020
Honkanen H, et al. Human Rhinoviruses Including Group C Are Common in Stool Samples of Young Finnish Children. J Clin Virol. 2013;56(3):250-4. PubMed PMID: 23273491.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human rhinoviruses including group C are common in stool samples of young Finnish children. AU - Honkanen,Hanna, AU - Oikarinen,Sami, AU - Peltonen,Pauliina, AU - Simell,Olli, AU - Ilonen,Jorma, AU - Veijola,Riitta, AU - Knip,Mikael, AU - Hyöty,Heikki, Y1 - 2012/12/27/ PY - 2012/09/19/received PY - 2012/11/08/revised PY - 2012/11/13/accepted PY - 2013/1/1/entrez PY - 2013/1/1/pubmed PY - 2013/7/5/medline SP - 250 EP - 4 JF - Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology JO - J Clin Virol VL - 56 IS - 3 N2 - BACKGROUND: Human rhinoviruses (HRVs) are common causes of viral respiratory infections. They have been widely studied in respiratory samples in hospital patient series but only a few studies have been performed to assess their occurrence in other sample types and their circulation in healthy children background population. OBJECTIVES: To analyze the frequency of HRVs in the background population in Finland by screening HRV RNA from stool samples longitudinally collected in a cohort of young children. STUDY DESIGN: Altogether 4184 stool samples were collected regularly from a cohort of children who were observed from birth. Samples were screened for the presence of RNA of HRVs using RT-PCR. HRV specific sequences were identified by sequencing the VP1 or VP4/VP2 coding region. Virus isolation was performed using four different cell lines and the result was confirmed by real time PCR. RESULTS: A total of 9% of the stool samples were positive for HRV RNA. Sequence analysis indicated that the most prevalent species was HRV-A, and the most prevalent serotype was HRV61. HRV-B and HRV-C species were also detected. One of the six tested rhinovirus positive samples retained its infectivity and was able to grow in RD and GMK cells. CONCLUSIONS: Our study shows that HRVs are frequently detected in the stool samples from the population of young children. We also show that HRV-C, which can cause severe illnesses in children, is commonly circulating in young children in Finland. SN - 1873-5967 UR - https://www.unboundmedicine.com/medline/citation/23273491/Human_rhinoviruses_including_group_C_are_common_in_stool_samples_of_young_Finnish_children_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1386-6532(12)00441-6 DB - PRIME DP - Unbound Medicine ER -