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Molecular diagnosis of echovirus 30 as the etiological agent in an outbreak of aseptic meningitis in Panama: May-June 2008.
J Infect Dev Ctries. 2012 Dec 15; 6(12):836-41.JI

Abstract

INTRODUCTION

Aseptic meningitis outbreaks are commonly caused by viral pathogens with enterovirus a common etiological agent. Between May and June of 2008, an outbreak of 173 cases of aseptic meningitis occurred in the Chiriqui Province of Panama. Molecular techniques were used to identify the etiological agent.

METHODOLOGY

Cerebrospinal fluid (CSF) samples from 75 patients were received at the Gorgas Memorial Institute for Health Studies. RNA extraction and one-step RT-PCR were performed on each sample to determine the presence of enterovirus. Thirty-four samples which were positive for enterovirus were subject to group-specific PCR, sequencing, and phylogenetic analysis to identify the etiological agent of the outbreak.

RESULTS

The CSF of 58 subjects was found positive for the enterovirus family using RT-PCR. Thirty-four samples were found to belong to the enterovirus B group. Phylogenetic analysis of four successfully sequenced samples revealed echovirus 30 as the etiological agent.

CONCLUSION

Echovirus 30 is reported as the likely cause of an outbreak of aseptic meningitis in Panama, the first since the 1980s.

Authors+Show Affiliations

Gorgas Memorial Institute for Health Studies, Panama City, Panama. amartineztm@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23276736

Citation

Martinez, Alexander A., et al. "Molecular Diagnosis of Echovirus 30 as the Etiological Agent in an Outbreak of Aseptic Meningitis in Panama: May-June 2008." Journal of Infection in Developing Countries, vol. 6, no. 12, 2012, pp. 836-41.
Martinez AA, Castillo J, Sanchez MC, et al. Molecular diagnosis of echovirus 30 as the etiological agent in an outbreak of aseptic meningitis in Panama: May-June 2008. J Infect Dev Ctries. 2012;6(12):836-41.
Martinez, A. A., Castillo, J., Sanchez, M. C., Zaldivar, Y., Mendoza, Y., Tribaldos, M., Acosta, P., Smith, R. E., & Pascale, J. M. (2012). Molecular diagnosis of echovirus 30 as the etiological agent in an outbreak of aseptic meningitis in Panama: May-June 2008. Journal of Infection in Developing Countries, 6(12), 836-41. https://doi.org/10.3855/jidc.2615
Martinez AA, et al. Molecular Diagnosis of Echovirus 30 as the Etiological Agent in an Outbreak of Aseptic Meningitis in Panama: May-June 2008. J Infect Dev Ctries. 2012 Dec 15;6(12):836-41. PubMed PMID: 23276736.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular diagnosis of echovirus 30 as the etiological agent in an outbreak of aseptic meningitis in Panama: May-June 2008. AU - Martinez,Alexander A, AU - Castillo,Juan, AU - Sanchez,Mirla C, AU - Zaldivar,Yamitzel, AU - Mendoza,Yaxelis, AU - Tribaldos,Maribel, AU - Acosta,Pablo, AU - Smith,Rebecca E, AU - Pascale,Juan Miguel, Y1 - 2012/12/15/ PY - 2012/03/02/received PY - 2012/08/26/accepted PY - 2012/12/09/revised PY - 2013/1/2/entrez PY - 2013/1/2/pubmed PY - 2013/6/12/medline SP - 836 EP - 41 JF - Journal of infection in developing countries JO - J Infect Dev Ctries VL - 6 IS - 12 N2 - INTRODUCTION: Aseptic meningitis outbreaks are commonly caused by viral pathogens with enterovirus a common etiological agent. Between May and June of 2008, an outbreak of 173 cases of aseptic meningitis occurred in the Chiriqui Province of Panama. Molecular techniques were used to identify the etiological agent. METHODOLOGY: Cerebrospinal fluid (CSF) samples from 75 patients were received at the Gorgas Memorial Institute for Health Studies. RNA extraction and one-step RT-PCR were performed on each sample to determine the presence of enterovirus. Thirty-four samples which were positive for enterovirus were subject to group-specific PCR, sequencing, and phylogenetic analysis to identify the etiological agent of the outbreak. RESULTS: The CSF of 58 subjects was found positive for the enterovirus family using RT-PCR. Thirty-four samples were found to belong to the enterovirus B group. Phylogenetic analysis of four successfully sequenced samples revealed echovirus 30 as the etiological agent. CONCLUSION: Echovirus 30 is reported as the likely cause of an outbreak of aseptic meningitis in Panama, the first since the 1980s. SN - 1972-2680 UR - https://www.unboundmedicine.com/medline/citation/23276736/Molecular_diagnosis_of_echovirus_30_as_the_etiological_agent_in_an_outbreak_of_aseptic_meningitis_in_Panama:_May_June_2008_ L2 - http://www.jidc.org/index.php/journal/article/view/23276736 DB - PRIME DP - Unbound Medicine ER -