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Comparison of ATR-FTIR spectra of porcine vaginal and buccal mucosa with ear skin and penetration analysis of drug and vehicle components into pig ear.
Eur J Pharm Sci. 2013 Dec 18; 50(5):595-600.EJ

Abstract

In the present study, porcine buccal and vaginal mucosae were successfully characterised by ATR-FTIR for the first time and compared to porcine ear skin. By analysing typical bands of the spectra, the structure of proteins and the lipid matrix were elucidated. According to the body site, differences in membrane permeability were detected when analysing the CH2-stretching and -scissoring vibrations. The results indicated a higher permeability for porcine vaginal and buccal tissue compared to skin. Furthermore, the influence of a lecithin-based microemulsion on the barrier properties of the above mentioned tissues was investigated by ATR-FTIR; the results revealed structural changes in all tissues. In addition, the ATR-FTIR technique was employed to semi-quantitatively analyse compounds directly on skin. To this end, tape stripping experiments were performed with a deuterated liposomal drug delivery system containing the model drug flufenamic acid. While the amount of penetrated deuterated liposomes was determined directly on skin samples by ATR-FTIR, the drug amount was analysed by HPLC after extraction of the tape strips since higher sensitivity was achieved in this fashion. Thus, it was possible to monitor the skin penetration of drug and vehicle simultaneously. Interestingly, the results indicated an independent drug penetration after release from the liposomal carrier system.

Authors+Show Affiliations

University of Vienna, Research Platform "Characterisation of Drug Delivery Systems on Skin and Investigation of Involved Mechanisms", Althanstraβe 14, 1090 Vienna, Austria.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23277290

Citation

Schwarz, Julia C., et al. "Comparison of ATR-FTIR Spectra of Porcine Vaginal and Buccal Mucosa With Ear Skin and Penetration Analysis of Drug and Vehicle Components Into Pig Ear." European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences, vol. 50, no. 5, 2013, pp. 595-600.
Schwarz JC, Pagitsch E, Valenta C. Comparison of ATR-FTIR spectra of porcine vaginal and buccal mucosa with ear skin and penetration analysis of drug and vehicle components into pig ear. Eur J Pharm Sci. 2013;50(5):595-600.
Schwarz, J. C., Pagitsch, E., & Valenta, C. (2013). Comparison of ATR-FTIR spectra of porcine vaginal and buccal mucosa with ear skin and penetration analysis of drug and vehicle components into pig ear. European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences, 50(5), 595-600. https://doi.org/10.1016/j.ejps.2012.12.020
Schwarz JC, Pagitsch E, Valenta C. Comparison of ATR-FTIR Spectra of Porcine Vaginal and Buccal Mucosa With Ear Skin and Penetration Analysis of Drug and Vehicle Components Into Pig Ear. Eur J Pharm Sci. 2013 Dec 18;50(5):595-600. PubMed PMID: 23277290.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of ATR-FTIR spectra of porcine vaginal and buccal mucosa with ear skin and penetration analysis of drug and vehicle components into pig ear. AU - Schwarz,Julia C, AU - Pagitsch,Elisabeth, AU - Valenta,Claudia, Y1 - 2012/12/28/ PY - 2012/11/27/received PY - 2012/12/19/revised PY - 2012/12/21/accepted PY - 2013/1/2/entrez PY - 2013/1/2/pubmed PY - 2014/5/23/medline KW - ATR–FTIR KW - Buccal mucosa KW - Liposome KW - Skin KW - Skin penetration KW - Vaginal mucosa SP - 595 EP - 600 JF - European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences JO - Eur J Pharm Sci VL - 50 IS - 5 N2 - In the present study, porcine buccal and vaginal mucosae were successfully characterised by ATR-FTIR for the first time and compared to porcine ear skin. By analysing typical bands of the spectra, the structure of proteins and the lipid matrix were elucidated. According to the body site, differences in membrane permeability were detected when analysing the CH2-stretching and -scissoring vibrations. The results indicated a higher permeability for porcine vaginal and buccal tissue compared to skin. Furthermore, the influence of a lecithin-based microemulsion on the barrier properties of the above mentioned tissues was investigated by ATR-FTIR; the results revealed structural changes in all tissues. In addition, the ATR-FTIR technique was employed to semi-quantitatively analyse compounds directly on skin. To this end, tape stripping experiments were performed with a deuterated liposomal drug delivery system containing the model drug flufenamic acid. While the amount of penetrated deuterated liposomes was determined directly on skin samples by ATR-FTIR, the drug amount was analysed by HPLC after extraction of the tape strips since higher sensitivity was achieved in this fashion. Thus, it was possible to monitor the skin penetration of drug and vehicle simultaneously. Interestingly, the results indicated an independent drug penetration after release from the liposomal carrier system. SN - 1879-0720 UR - https://www.unboundmedicine.com/medline/citation/23277290/Comparison_of_ATR_FTIR_spectra_of_porcine_vaginal_and_buccal_mucosa_with_ear_skin_and_penetration_analysis_of_drug_and_vehicle_components_into_pig_ear_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0928-0987(12)00494-0 DB - PRIME DP - Unbound Medicine ER -