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Detection of lead (II) with a "turn-on" fluorescent biosensor based on energy transfer from CdSe/ZnS quantum dots to graphene oxide.
Biosens Bioelectron. 2013 May 15; 43:69-74.BB

Abstract

Graphene oxide (GO) sheets are mixed with the aptamer-functionalized CdSe/ZnS quantum dots (QDs). Consequently, the aptamer-conjugated QDs bind to the GO sheets to form a GO/aptamer-QD ensemble, which enables the energy transfer from the QDs to the GO sheets, quenching the fluorescence of QDs. The GO/aptamer-QD ensemble assay acts as a "turn-on" fluorescent sensor for Pb(2+) detection. When Pb(2+) ions are present in the assay, the interaction of Pb(2+) with the aptamer induces a conformational change in the aptamer, leading to the formation of a G-quadruplex/Pb(2+) complex. As a result, the QDs that are linked to the G-quadruplex/Pb(2+) complex are detached from the GO sheet, which "turns on" the fluorescence of the QDs. This sensor exhibits a limit of detection of 90pM and excellent selectivity toward Pb(2+) over a wide range of metal ions. The experiments have provided direct evidence that the fluorescence of QDs is quenched by GO via the nano-metal surface energy transfer (NSET) mechanism rather than the conventional Förster resonance energy transfer (FRET) process.

Authors+Show Affiliations

Department of Mechanical and Aerospace Engineering, West Virginia University, Morgantown, WV 26506-6106, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

23277342

Citation

Li, Ming, et al. "Detection of Lead (II) With a "turn-on" Fluorescent Biosensor Based On Energy Transfer From CdSe/ZnS Quantum Dots to Graphene Oxide." Biosensors & Bioelectronics, vol. 43, 2013, pp. 69-74.
Li M, Zhou X, Guo S, et al. Detection of lead (II) with a "turn-on" fluorescent biosensor based on energy transfer from CdSe/ZnS quantum dots to graphene oxide. Biosens Bioelectron. 2013;43:69-74.
Li, M., Zhou, X., Guo, S., & Wu, N. (2013). Detection of lead (II) with a "turn-on" fluorescent biosensor based on energy transfer from CdSe/ZnS quantum dots to graphene oxide. Biosensors & Bioelectronics, 43, 69-74. https://doi.org/10.1016/j.bios.2012.11.039
Li M, et al. Detection of Lead (II) With a "turn-on" Fluorescent Biosensor Based On Energy Transfer From CdSe/ZnS Quantum Dots to Graphene Oxide. Biosens Bioelectron. 2013 May 15;43:69-74. PubMed PMID: 23277342.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of lead (II) with a "turn-on" fluorescent biosensor based on energy transfer from CdSe/ZnS quantum dots to graphene oxide. AU - Li,Ming, AU - Zhou,Xuejiao, AU - Guo,Shouwu, AU - Wu,Nianqiang, Y1 - 2012/12/07/ PY - 2012/09/25/received PY - 2012/11/20/revised PY - 2012/11/30/accepted PY - 2013/1/2/entrez PY - 2013/1/2/pubmed PY - 2013/8/22/medline SP - 69 EP - 74 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 43 N2 - Graphene oxide (GO) sheets are mixed with the aptamer-functionalized CdSe/ZnS quantum dots (QDs). Consequently, the aptamer-conjugated QDs bind to the GO sheets to form a GO/aptamer-QD ensemble, which enables the energy transfer from the QDs to the GO sheets, quenching the fluorescence of QDs. The GO/aptamer-QD ensemble assay acts as a "turn-on" fluorescent sensor for Pb(2+) detection. When Pb(2+) ions are present in the assay, the interaction of Pb(2+) with the aptamer induces a conformational change in the aptamer, leading to the formation of a G-quadruplex/Pb(2+) complex. As a result, the QDs that are linked to the G-quadruplex/Pb(2+) complex are detached from the GO sheet, which "turns on" the fluorescence of the QDs. This sensor exhibits a limit of detection of 90pM and excellent selectivity toward Pb(2+) over a wide range of metal ions. The experiments have provided direct evidence that the fluorescence of QDs is quenched by GO via the nano-metal surface energy transfer (NSET) mechanism rather than the conventional Förster resonance energy transfer (FRET) process. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/23277342/Detection_of_lead__II__with_a_"turn_on"_fluorescent_biosensor_based_on_energy_transfer_from_CdSe/ZnS_quantum_dots_to_graphene_oxide_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(12)00852-4 DB - PRIME DP - Unbound Medicine ER -