TY - JOUR T1 - Evaluation of the MYC_CANFA gene in Chinese Shar Peis with cobalamin deficiency. AU - Grützner,Niels, AU - Bishop,Micah A, AU - Suchodolski,Jan S, AU - Steiner,Jörg M, Y1 - 2012/12/31/ PY - 2013/1/3/entrez PY - 2013/1/3/pubmed PY - 2013/8/29/medline SP - 61 EP - 5 JF - Veterinary clinical pathology JO - Vet Clin Pathol VL - 42 IS - 1 N2 - BACKGROUND: A recent genome-wide scan using the canine minimal screening set 2 (MSS-2) showed that cobalamin deficiency appears to be hereditary in Chinese Shar Peis and is linked to the microsatellite markers DTR13.6 and REN13N11 on canine chromosome 13. OBJECTIVE: The goal of this study was to evaluate the MYC_CANFA gene, which is the closest known gene with a distance of approximately 0.06 megabases (Mb) to the microsatellite marker DTR13.6, for any mutations in this breed. METHODS: Microsatellite markers (Myc and G15987) for genotyping and primers for sequencing were used to evaluate the MYC_CANFA gene. The genotype and gene sequence were compared between cobalamin-deficient Shar Peis, Shar Peis with normal serum cobalamin concentrations, and the DNA sequences published as part of the Ensemble Genomic map. RESULTS: Neither the microsatellite markers (Myc and G15987) nor the sequences of the MYC_CANFA gene showed a significant difference among both groups of Shar Peis and the published canine DNA sequence. CONCLUSIONS: The data presented here suggest that cobalamin deficiency in Shar Peis is not related to any mutations of the MYC_CANFA gene according to the genotyping and sequencing results in this study. Further investigations are warranted to find a potential genomic locus in proximity to DTR13.6 and REN13N11 that shows mutations in cobalamin-deficient Shar Peis. SN - 1939-165X UR - https://www.unboundmedicine.com/medline/citation/23278253/Evaluation_of_the_MYC_CANFA_gene_in_Chinese_Shar_Peis_with_cobalamin_deficiency_ L2 - https://doi.org/10.1111/vcp.12010 DB - PRIME DP - Unbound Medicine ER -