Tags

Type your tag names separated by a space and hit enter

The cysteine proteinases of the pineapple plant.
Biochem J. 1990 Mar 15; 266(3):869-75.BJ

Abstract

The pineapple plant (Ananas comosus) was shown to contain at least four distinct cysteine proteinases, which were purified by a procedure involving active-site-directed affinity chromatography. The major proteinase present in extracts of plant stem was stem bromelain, whilst fruit bromelain was the major proteinase in the fruit. Two additional cysteine proteinases were detected only in the stem: these were ananain and a previously undescribed enzyme that we have called comosain. Stem bromelain, fruit bromelain and ananain were shown to be immunologically distinct. Enzymic characterization revealed differences in both substrate-specificities and inhibition profiles. A study of the cysteine proteinase derived from the related bromeliad Bromelia pinguin (pinguinain) indicated that in many respects it was similar to fruit bromelain, although it was found to be immunologically distinct.

Authors+Show Affiliations

Department of Biochemistry, Strangeways Research Laboratory, Cambridge, U.K.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2327970

Citation

Rowan, A D., et al. "The Cysteine Proteinases of the Pineapple Plant." The Biochemical Journal, vol. 266, no. 3, 1990, pp. 869-75.
Rowan AD, Buttle DJ, Barrett AJ. The cysteine proteinases of the pineapple plant. Biochem J. 1990;266(3):869-75.
Rowan, A. D., Buttle, D. J., & Barrett, A. J. (1990). The cysteine proteinases of the pineapple plant. The Biochemical Journal, 266(3), 869-75.
Rowan AD, Buttle DJ, Barrett AJ. The Cysteine Proteinases of the Pineapple Plant. Biochem J. 1990 Mar 15;266(3):869-75. PubMed PMID: 2327970.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The cysteine proteinases of the pineapple plant. AU - Rowan,A D, AU - Buttle,D J, AU - Barrett,A J, PY - 1990/3/15/pubmed PY - 1990/3/15/medline PY - 1990/3/15/entrez SP - 869 EP - 75 JF - The Biochemical journal JO - Biochem. J. VL - 266 IS - 3 N2 - The pineapple plant (Ananas comosus) was shown to contain at least four distinct cysteine proteinases, which were purified by a procedure involving active-site-directed affinity chromatography. The major proteinase present in extracts of plant stem was stem bromelain, whilst fruit bromelain was the major proteinase in the fruit. Two additional cysteine proteinases were detected only in the stem: these were ananain and a previously undescribed enzyme that we have called comosain. Stem bromelain, fruit bromelain and ananain were shown to be immunologically distinct. Enzymic characterization revealed differences in both substrate-specificities and inhibition profiles. A study of the cysteine proteinase derived from the related bromeliad Bromelia pinguin (pinguinain) indicated that in many respects it was similar to fruit bromelain, although it was found to be immunologically distinct. SN - 0264-6021 UR - https://www.unboundmedicine.com/medline/citation/2327970/The_cysteine_proteinases_of_the_pineapple_plant_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/2327970/ DB - PRIME DP - Unbound Medicine ER -