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A comparative study on the inhibitory effects of different parts and chemical constituents of pomegranate on α-amylase and α-glucosidase.
Phytother Res. 2013 Nov; 27(11):1614-20.PR

Abstract

Pomegranate has been documented for the management of diabetes in Unani and Chinese medicine. This study compared the effects of the extracts of different pomegranate parts, including juice, peels, seeds and flowers, on carbohydrate digestive enzymes (α-amylase and α-glucosidase) in vitro. The methanolic flower extract inhibited α-amylase and α-glucosidase, while the methanolic peel extract inhibited α-glucosidase selectively. The most active flower extract was subjected to water-ethyl acetate partition. The ethyl acetate fraction was more potent than the water fraction in inhibiting both enzymes. Gallic acid and ellagic acid also showed selective inhibition against α-glucosidase, and their presence in the ethyl acetate fraction was confirmed by HPLC-DAD and HPLC-HESI-MS. Our findings suggest that the inhibition of carbohydrate digestive enzymes and their phenolic content may contribute to the anti-hyperglycaemic effects of pomegranate flower and peel, and support their claims in diabetes.

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Authors+Show Affiliations

Kam A
Faculty of Pharmacy, University of Sydney, NSW 2006, Australia.
Li KM
No affiliation info available
Razmovski-Naumovski V
No affiliation info available
Nammi S
No affiliation info available
Shi J
No affiliation info available
Chan K
No affiliation info available
Li GQ
No affiliation info available

MeSH

AnimalsEllagic AcidEnzyme InhibitorsFlowersFruitGallic AcidGlycoside Hydrolase InhibitorsHypoglycemic AgentsLythraceaePlant ExtractsRatsSeedsSwinealpha-Amylases

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23280757

Citation

Kam, Antony, et al. "A Comparative Study On the Inhibitory Effects of Different Parts and Chemical Constituents of Pomegranate On Α-amylase and Α-glucosidase." Phytotherapy Research : PTR, vol. 27, no. 11, 2013, pp. 1614-20.
Kam A, Li KM, Razmovski-Naumovski V, et al. A comparative study on the inhibitory effects of different parts and chemical constituents of pomegranate on α-amylase and α-glucosidase. Phytother Res. 2013;27(11):1614-20.
Kam, A., Li, K. M., Razmovski-Naumovski, V., Nammi, S., Shi, J., Chan, K., & Li, G. Q. (2013). A comparative study on the inhibitory effects of different parts and chemical constituents of pomegranate on α-amylase and α-glucosidase. Phytotherapy Research : PTR, 27(11), 1614-20. https://doi.org/10.1002/ptr.4913
Kam A, et al. A Comparative Study On the Inhibitory Effects of Different Parts and Chemical Constituents of Pomegranate On Α-amylase and Α-glucosidase. Phytother Res. 2013;27(11):1614-20. PubMed PMID: 23280757.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A comparative study on the inhibitory effects of different parts and chemical constituents of pomegranate on α-amylase and α-glucosidase. AU - Kam,Antony, AU - Li,Kong M, AU - Razmovski-Naumovski,Valentina, AU - Nammi,Srinivas, AU - Shi,Jeffrey, AU - Chan,Kelvin, AU - Li,George Q, Y1 - 2012/12/19/ PY - 2011/08/19/received PY - 2012/11/15/revised PY - 2012/11/25/accepted PY - 2013/1/3/entrez PY - 2013/1/3/pubmed PY - 2014/4/24/medline KW - Punica granatum KW - diabetes KW - pomegranate KW - α-amylase KW - α-glucosidase SP - 1614 EP - 20 JF - Phytotherapy research : PTR JO - Phytother Res VL - 27 IS - 11 N2 - Pomegranate has been documented for the management of diabetes in Unani and Chinese medicine. This study compared the effects of the extracts of different pomegranate parts, including juice, peels, seeds and flowers, on carbohydrate digestive enzymes (α-amylase and α-glucosidase) in vitro. The methanolic flower extract inhibited α-amylase and α-glucosidase, while the methanolic peel extract inhibited α-glucosidase selectively. The most active flower extract was subjected to water-ethyl acetate partition. The ethyl acetate fraction was more potent than the water fraction in inhibiting both enzymes. Gallic acid and ellagic acid also showed selective inhibition against α-glucosidase, and their presence in the ethyl acetate fraction was confirmed by HPLC-DAD and HPLC-HESI-MS. Our findings suggest that the inhibition of carbohydrate digestive enzymes and their phenolic content may contribute to the anti-hyperglycaemic effects of pomegranate flower and peel, and support their claims in diabetes. SN - 1099-1573 UR - https://www.unboundmedicine.com/medline/citation/23280757/A_comparative_study_on_the_inhibitory_effects_of_different_parts_and_chemical_constituents_of_pomegranate_on_α_amylase_and_α_glucosidase_ L2 - https://doi.org/10.1002/ptr.4913 DB - PRIME DP - Unbound Medicine ER -