Tags

Type your tag names separated by a space and hit enter

Genetic variation among Panton-Valentine leukocidin-encoding bacteriophages in Staphylococcus aureus clonal complex 30 strains.
J Clin Microbiol 2013; 51(3):914-9JC

Abstract

Clonal complex 30 (CC30), one of the major Staphylococcus aureus lineages, has caused extensive hospital-acquired and community-acquired infections worldwide. Recent comparative genomics studies have demonstrated that three CC30 clones-phage type 80/81, Southwest Pacific (SWP), and contemporary EMRSA-16 associated (Con) strains-shared a recent common ancestor more than 100 years ago. Panton-Valentine leukocidin (PVL), a bacteriophage encoded toxin that has been epidemiologically linked with community-associated methicillin-resistant S. aureus (CA-MRSA), has frequently been identified in CC30 clones, although the pvl gene variation and distribution of PVL-encoding phages are poorly understood. We determined here the distribution of PVL phages, PVL gene sequences, and chromosomal phage insertion sites in 52 S. aureus CC30 PVL-harboring isolates, collected from four continents over a 75-year period. Our results indicate that PVL phages with icosahedral heads, including Φ108PVL and ΦPVL, were mainly associated with phage 80/81 strains, whereas phages with elongated heads were predominantly found in SWP (ΦSa2958 and ΦTCH60) and Con (ΦSa2USA) strains. Nine single-nucleotide polymorphisms were identified in the lukSF-PV gene, with six isolates harboring the R variant that has been previously associated with CA-MRSA strains. Interestingly, all six R variant strains belonged to the same Con CC30 clone and carried a ΦSa2USA-like phage. Similar chromosomal phage insertion sites were also identified in all 52 PVL-harboring CC30 strains. These analyses provide important insights into the microepidemiology of PVL-harboring CC30 strains, while the discovery of ΦSa2USA-associated R variant strains sheds further light on the evolution of PVL-positive CA-MRSA.

Authors+Show Affiliations

Public Health Research Institute Center, International Center for Public Health, University of Medicine and Dentistry of New Jersey, Newark, New Jersey, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23284024

Citation

Chen, Liang, et al. "Genetic Variation Among Panton-Valentine Leukocidin-encoding Bacteriophages in Staphylococcus Aureus Clonal Complex 30 Strains." Journal of Clinical Microbiology, vol. 51, no. 3, 2013, pp. 914-9.
Chen L, Chavda KD, Solanki M, et al. Genetic variation among Panton-Valentine leukocidin-encoding bacteriophages in Staphylococcus aureus clonal complex 30 strains. J Clin Microbiol. 2013;51(3):914-9.
Chen, L., Chavda, K. D., Solanki, M., Mediavilla, J. R., Mathema, B., Schlievert, P. M., & Kreiswirth, B. N. (2013). Genetic variation among Panton-Valentine leukocidin-encoding bacteriophages in Staphylococcus aureus clonal complex 30 strains. Journal of Clinical Microbiology, 51(3), pp. 914-9. doi:10.1128/JCM.03015-12.
Chen L, et al. Genetic Variation Among Panton-Valentine Leukocidin-encoding Bacteriophages in Staphylococcus Aureus Clonal Complex 30 Strains. J Clin Microbiol. 2013;51(3):914-9. PubMed PMID: 23284024.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic variation among Panton-Valentine leukocidin-encoding bacteriophages in Staphylococcus aureus clonal complex 30 strains. AU - Chen,Liang, AU - Chavda,Kalyan D, AU - Solanki,Mihir, AU - Mediavilla,José R, AU - Mathema,Barun, AU - Schlievert,Patrick M, AU - Kreiswirth,Barry N, Y1 - 2013/01/02/ PY - 2013/1/4/entrez PY - 2013/1/4/pubmed PY - 2013/7/31/medline SP - 914 EP - 9 JF - Journal of clinical microbiology JO - J. Clin. Microbiol. VL - 51 IS - 3 N2 - Clonal complex 30 (CC30), one of the major Staphylococcus aureus lineages, has caused extensive hospital-acquired and community-acquired infections worldwide. Recent comparative genomics studies have demonstrated that three CC30 clones-phage type 80/81, Southwest Pacific (SWP), and contemporary EMRSA-16 associated (Con) strains-shared a recent common ancestor more than 100 years ago. Panton-Valentine leukocidin (PVL), a bacteriophage encoded toxin that has been epidemiologically linked with community-associated methicillin-resistant S. aureus (CA-MRSA), has frequently been identified in CC30 clones, although the pvl gene variation and distribution of PVL-encoding phages are poorly understood. We determined here the distribution of PVL phages, PVL gene sequences, and chromosomal phage insertion sites in 52 S. aureus CC30 PVL-harboring isolates, collected from four continents over a 75-year period. Our results indicate that PVL phages with icosahedral heads, including Φ108PVL and ΦPVL, were mainly associated with phage 80/81 strains, whereas phages with elongated heads were predominantly found in SWP (ΦSa2958 and ΦTCH60) and Con (ΦSa2USA) strains. Nine single-nucleotide polymorphisms were identified in the lukSF-PV gene, with six isolates harboring the R variant that has been previously associated with CA-MRSA strains. Interestingly, all six R variant strains belonged to the same Con CC30 clone and carried a ΦSa2USA-like phage. Similar chromosomal phage insertion sites were also identified in all 52 PVL-harboring CC30 strains. These analyses provide important insights into the microepidemiology of PVL-harboring CC30 strains, while the discovery of ΦSa2USA-associated R variant strains sheds further light on the evolution of PVL-positive CA-MRSA. SN - 1098-660X UR - https://www.unboundmedicine.com/medline/citation/23284024/Genetic_variation_among_Panton_Valentine_leukocidin_encoding_bacteriophages_in_Staphylococcus_aureus_clonal_complex_30_strains_ L2 - http://jcm.asm.org/cgi/pmidlookup?view=long&pmid=23284024 DB - PRIME DP - Unbound Medicine ER -