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Oxidative cell injury as a predictor of endometriosis progression.

Abstract

BACKGROUND

There is increasing evidence that oxidative stress is one of the key factors for progression of endometriosis. In this prospective controlled trial, we measured 6 different biomarkers of oxidative stress targeting protein, lipid, and DNA to quantify the severity and progression of endometriosis and establish a diagnostic marker for the disease.

METHODS

A total of 62 consecutive patients were identified and enrolled in this study. After exclusion criteria, 44 patients were allocated to 3 groups: stage I/II (n = 14), stage III/IV (n = 16), and a control group (n = 14). The levels of 8-hydroxy-2-deoxyguanosine (8-OHdG), 8-oxoguanine DNA glycosylase (OGG1), protein carbonyl (PC), lipid peroxidation (LPO), reactive oxygen species (ROS), and total antioxidant capacity (TAC) were accessed in peritoneal fluid and tissue.

RESULTS

Significantly higher levels of 8-OHdG and PC were seen in patients with endometriosis, in addition OGG1 expression was found to be significantly lower in patients with endometriosis (P < .001, P = .001, P = .033, respectively); ROS, TAC, and LPO were similar in stages I/II, stages III/IV, and control group. A predictive model was built using multivariable analyses and receiver-operating characteristics curves. The ability to predict and distinguish between patients without endometriosis, stage I/II endometriosis, and stage III/IV was very high. This model was highly discriminatory and had a concordance index of 0.87.

CONCLUSION

In this cohort, higher DNA damage and lower DNA repair activity was related to endometriosis progression. Our results indicate that oxidative stress as a biomarker of cell injury can be used as a reliable quantitative test of endometriosis severity.

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  • Authors+Show Affiliations

    ,

    1Center for Reproductive Medicine, Obstetrics and Gynecology and Women's Health Institute, Cleveland Clinic, Cleveland, OH 44195, USA.

    , , , ,

    Source

    MeSH

    Adult
    Ascitic Fluid
    Biomarkers
    Biopsy
    Case-Control Studies
    DNA Damage
    DNA Glycosylases
    DNA Repair
    Deoxyguanosine
    Disease Progression
    Endometriosis
    Female
    Humans
    Immunohistochemistry
    Lipid Peroxidation
    Logistic Models
    Middle Aged
    Multivariate Analysis
    Odds Ratio
    Oxidative Stress
    Predictive Value of Tests
    Protein Carbonylation
    ROC Curve
    Reactive Oxygen Species
    Reproducibility of Results
    Severity of Illness Index

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    23287096

    Citation

    Carvalho, Luiz Fernando Pina, et al. "Oxidative Cell Injury as a Predictor of Endometriosis Progression." Reproductive Sciences (Thousand Oaks, Calif.), vol. 20, no. 6, 2013, pp. 688-98.
    Carvalho LF, Abrão MS, Biscotti C, et al. Oxidative cell injury as a predictor of endometriosis progression. Reprod Sci. 2013;20(6):688-98.
    Carvalho, L. F., Abrão, M. S., Biscotti, C., Sharma, R., Nutter, B., & Falcone, T. (2013). Oxidative cell injury as a predictor of endometriosis progression. Reproductive Sciences (Thousand Oaks, Calif.), 20(6), pp. 688-98. doi:10.1177/1933719112466301.
    Carvalho LF, et al. Oxidative Cell Injury as a Predictor of Endometriosis Progression. Reprod Sci. 2013;20(6):688-98. PubMed PMID: 23287096.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Oxidative cell injury as a predictor of endometriosis progression. AU - Carvalho,Luiz Fernando Pina, AU - Abrão,Mauricio Simões, AU - Biscotti,Charles, AU - Sharma,Rakesh, AU - Nutter,Benjamin, AU - Falcone,Tommaso, Y1 - 2013/01/03/ PY - 2013/1/5/entrez PY - 2013/1/5/pubmed PY - 2013/12/16/medline KW - DNA damage: 8-hydroxy-2-deoxyguanosine KW - cell toxicity KW - endometriosis KW - endometriosis progression KW - oxidative stress SP - 688 EP - 98 JF - Reproductive sciences (Thousand Oaks, Calif.) JO - Reprod Sci VL - 20 IS - 6 N2 - BACKGROUND: There is increasing evidence that oxidative stress is one of the key factors for progression of endometriosis. In this prospective controlled trial, we measured 6 different biomarkers of oxidative stress targeting protein, lipid, and DNA to quantify the severity and progression of endometriosis and establish a diagnostic marker for the disease. METHODS: A total of 62 consecutive patients were identified and enrolled in this study. After exclusion criteria, 44 patients were allocated to 3 groups: stage I/II (n = 14), stage III/IV (n = 16), and a control group (n = 14). The levels of 8-hydroxy-2-deoxyguanosine (8-OHdG), 8-oxoguanine DNA glycosylase (OGG1), protein carbonyl (PC), lipid peroxidation (LPO), reactive oxygen species (ROS), and total antioxidant capacity (TAC) were accessed in peritoneal fluid and tissue. RESULTS: Significantly higher levels of 8-OHdG and PC were seen in patients with endometriosis, in addition OGG1 expression was found to be significantly lower in patients with endometriosis (P < .001, P = .001, P = .033, respectively); ROS, TAC, and LPO were similar in stages I/II, stages III/IV, and control group. A predictive model was built using multivariable analyses and receiver-operating characteristics curves. The ability to predict and distinguish between patients without endometriosis, stage I/II endometriosis, and stage III/IV was very high. This model was highly discriminatory and had a concordance index of 0.87. CONCLUSION: In this cohort, higher DNA damage and lower DNA repair activity was related to endometriosis progression. Our results indicate that oxidative stress as a biomarker of cell injury can be used as a reliable quantitative test of endometriosis severity. SN - 1933-7205 UR - https://www.unboundmedicine.com/medline/citation/23287096/Oxidative_cell_injury_as_a_predictor_of_endometriosis_progression_ L2 - http://journals.sagepub.com/doi/full/10.1177/1933719112466301?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -