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Diversity and relative levels of actinidin, kiwellin, and thaumatin-like allergens in 15 varieties of kiwifruit (Actinidia).
J Agric Food Chem 2013; 61(3):728-39JA

Abstract

In the last 30 years the incidence of kiwifruit allergy has increased with the three major allergenic proteins being identified as actinidin, kiwellin, and thaumatin-like protein (TLP). We report wide variation in the levels of actinidin and TLP in 15 kiwifruit varieties from the four most widely cultivated Actinidia species. Acidic and basic isoforms of actinidin were identified in Actinidia deliciosa 'Hayward' and Actinidia arguta 'Hortgem Tahi', while only a basic isoform of actinidin was identified in Actinidia chinensis 'Hort16A'. One isoform each of kiwellin and TLP were identified in ripe fruit. The cysteine protease activity of actinidin correlated with protein levels in all species except A. arguta. Protein modeling suggested that modifications to the S2 binding pocket influenced substrate specificity of the A. arguta enzyme. Our results indicate that care is necessary when extrapolating allergenicity results from single varieties to others within the same and between different Actinidia species.

Authors+Show Affiliations

The New Zealand Institute for Plant & Food Research Limited (PFR), Auckland, New Zealand.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23289429

Citation

Maddumage, Ratnasiri, et al. "Diversity and Relative Levels of Actinidin, Kiwellin, and Thaumatin-like Allergens in 15 Varieties of Kiwifruit (Actinidia)." Journal of Agricultural and Food Chemistry, vol. 61, no. 3, 2013, pp. 728-39.
Maddumage R, Nieuwenhuizen NJ, Bulley SM, et al. Diversity and relative levels of actinidin, kiwellin, and thaumatin-like allergens in 15 varieties of kiwifruit (Actinidia). J Agric Food Chem. 2013;61(3):728-39.
Maddumage, R., Nieuwenhuizen, N. J., Bulley, S. M., Cooney, J. M., Green, S. A., & Atkinson, R. G. (2013). Diversity and relative levels of actinidin, kiwellin, and thaumatin-like allergens in 15 varieties of kiwifruit (Actinidia). Journal of Agricultural and Food Chemistry, 61(3), pp. 728-39. doi:10.1021/jf304289f.
Maddumage R, et al. Diversity and Relative Levels of Actinidin, Kiwellin, and Thaumatin-like Allergens in 15 Varieties of Kiwifruit (Actinidia). J Agric Food Chem. 2013 Jan 23;61(3):728-39. PubMed PMID: 23289429.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diversity and relative levels of actinidin, kiwellin, and thaumatin-like allergens in 15 varieties of kiwifruit (Actinidia). AU - Maddumage,Ratnasiri, AU - Nieuwenhuizen,Niels J, AU - Bulley,Sean M, AU - Cooney,Janine M, AU - Green,Sol A, AU - Atkinson,Ross G, Y1 - 2013/01/14/ PY - 2013/1/8/entrez PY - 2013/1/8/pubmed PY - 2014/1/22/medline SP - 728 EP - 39 JF - Journal of agricultural and food chemistry JO - J. Agric. Food Chem. VL - 61 IS - 3 N2 - In the last 30 years the incidence of kiwifruit allergy has increased with the three major allergenic proteins being identified as actinidin, kiwellin, and thaumatin-like protein (TLP). We report wide variation in the levels of actinidin and TLP in 15 kiwifruit varieties from the four most widely cultivated Actinidia species. Acidic and basic isoforms of actinidin were identified in Actinidia deliciosa 'Hayward' and Actinidia arguta 'Hortgem Tahi', while only a basic isoform of actinidin was identified in Actinidia chinensis 'Hort16A'. One isoform each of kiwellin and TLP were identified in ripe fruit. The cysteine protease activity of actinidin correlated with protein levels in all species except A. arguta. Protein modeling suggested that modifications to the S2 binding pocket influenced substrate specificity of the A. arguta enzyme. Our results indicate that care is necessary when extrapolating allergenicity results from single varieties to others within the same and between different Actinidia species. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/23289429/Diversity_and_relative_levels_of_actinidin_kiwellin_and_thaumatin_like_allergens_in_15_varieties_of_kiwifruit__Actinidia__ L2 - https://dx.doi.org/10.1021/jf304289f DB - PRIME DP - Unbound Medicine ER -