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Are sperm DNA fragmentation, hyperactivation, and hyaluronan-binding ability predictive for fertilization and embryo development in in vitro fertilization and intracytoplasmic sperm injection?
Fertil Steril 2013; 99(5):1233-41FS

Abstract

OBJECTIVE

To determine the diagnostic value of the following sperm function tests in predicting the fertilizing ability of spermatozoa in conventional in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI): hyaluronan-binding assay (HBA), DNA fragmentation (Halosperm), and hyperactivity.

DESIGN

Prospective study.

SETTING

University medical center.

PATIENT(S)

133 couples undergoing infertility treatment with IVF/ICSI.

INTERVENTION(S)

Analysis of sperm DNA fragmentation, hyaluronan-binding ability, and hyperactivation on washed semen samples used for the insemination of oocytes.

MAIN OUTCOME MEASURE(S)

Correlation between the results of sperm function tests and the fertilization rate (FR) or embryo quality (EQ) after IVF and ICSI. Comparison of the sperm DNA fragmentation, hyperactivation, and hyaluronan binding ability between cycles with less than 50% (group 1) and more than 50% (group 2) of oocytes fertilized after IVF.

RESULT(S)

Both FR and EQ in IVF cycles negatively correlated with sperm DNA fragmentation. Furthermore, a positive correlation was observed between FR and hyaluronan-binding ability or induced hyperactivity. The semen samples from the IVF cycles with low FR (group 1) were characterized by statistically significantly higher sperm DNA fragmentation and lower hyaluronan-binding ability in comparison with semen samples from the group with high levels of fertilization (group 2). In ICSI cycles, no relationship was found between sperm function tests and FR or EQ.

CONCLUSION(S)

The Halosperm test, the HBA test, and induced hyperactivity are useful in predicting the ability of spermatozoa to fertilize oocytes in IVF and are helpful in distinguishing semen samples suitable for IVF or ICSI.

Authors+Show Affiliations

Department of Reproductive Medicine and Gynaecologic Endocrinology, University Medical Centre, Maribor, Slovenia. breznik.barbara@siol.netNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23290739

Citation

Pregl Breznik, Barbara, et al. "Are Sperm DNA Fragmentation, Hyperactivation, and Hyaluronan-binding Ability Predictive for Fertilization and Embryo Development in in Vitro Fertilization and Intracytoplasmic Sperm Injection?" Fertility and Sterility, vol. 99, no. 5, 2013, pp. 1233-41.
Pregl Breznik B, Kovačič B, Vlaisavljević V. Are sperm DNA fragmentation, hyperactivation, and hyaluronan-binding ability predictive for fertilization and embryo development in in vitro fertilization and intracytoplasmic sperm injection? Fertil Steril. 2013;99(5):1233-41.
Pregl Breznik, B., Kovačič, B., & Vlaisavljević, V. (2013). Are sperm DNA fragmentation, hyperactivation, and hyaluronan-binding ability predictive for fertilization and embryo development in in vitro fertilization and intracytoplasmic sperm injection? Fertility and Sterility, 99(5), pp. 1233-41. doi:10.1016/j.fertnstert.2012.11.048.
Pregl Breznik B, Kovačič B, Vlaisavljević V. Are Sperm DNA Fragmentation, Hyperactivation, and Hyaluronan-binding Ability Predictive for Fertilization and Embryo Development in in Vitro Fertilization and Intracytoplasmic Sperm Injection. Fertil Steril. 2013;99(5):1233-41. PubMed PMID: 23290739.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Are sperm DNA fragmentation, hyperactivation, and hyaluronan-binding ability predictive for fertilization and embryo development in in vitro fertilization and intracytoplasmic sperm injection? AU - Pregl Breznik,Barbara, AU - Kovačič,Borut, AU - Vlaisavljević,Veljko, Y1 - 2013/01/03/ PY - 2012/08/11/received PY - 2012/11/22/revised PY - 2012/11/26/accepted PY - 2013/1/8/entrez PY - 2013/1/8/pubmed PY - 2013/7/3/medline SP - 1233 EP - 41 JF - Fertility and sterility JO - Fertil. Steril. VL - 99 IS - 5 N2 - OBJECTIVE: To determine the diagnostic value of the following sperm function tests in predicting the fertilizing ability of spermatozoa in conventional in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI): hyaluronan-binding assay (HBA), DNA fragmentation (Halosperm), and hyperactivity. DESIGN: Prospective study. SETTING: University medical center. PATIENT(S): 133 couples undergoing infertility treatment with IVF/ICSI. INTERVENTION(S): Analysis of sperm DNA fragmentation, hyaluronan-binding ability, and hyperactivation on washed semen samples used for the insemination of oocytes. MAIN OUTCOME MEASURE(S): Correlation between the results of sperm function tests and the fertilization rate (FR) or embryo quality (EQ) after IVF and ICSI. Comparison of the sperm DNA fragmentation, hyperactivation, and hyaluronan binding ability between cycles with less than 50% (group 1) and more than 50% (group 2) of oocytes fertilized after IVF. RESULT(S): Both FR and EQ in IVF cycles negatively correlated with sperm DNA fragmentation. Furthermore, a positive correlation was observed between FR and hyaluronan-binding ability or induced hyperactivity. The semen samples from the IVF cycles with low FR (group 1) were characterized by statistically significantly higher sperm DNA fragmentation and lower hyaluronan-binding ability in comparison with semen samples from the group with high levels of fertilization (group 2). In ICSI cycles, no relationship was found between sperm function tests and FR or EQ. CONCLUSION(S): The Halosperm test, the HBA test, and induced hyperactivity are useful in predicting the ability of spermatozoa to fertilize oocytes in IVF and are helpful in distinguishing semen samples suitable for IVF or ICSI. SN - 1556-5653 UR - https://www.unboundmedicine.com/medline/citation/23290739/Are_sperm_DNA_fragmentation_hyperactivation_and_hyaluronan_binding_ability_predictive_for_fertilization_and_embryo_development_in_in_vitro_fertilization_and_intracytoplasmic_sperm_injection L2 - https://linkinghub.elsevier.com/retrieve/pii/S0015-0282(12)02460-0 DB - PRIME DP - Unbound Medicine ER -