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Hedyotis diffusa Willd extract suppresses Sonic hedgehog signaling leading to the inhibition of colorectal cancer angiogenesis.
Int J Oncol 2013; 42(2):651-6IJ

Abstract

Sonic hedgehog (SHH) signaling pathway promotes the process of angiogenesis, contributing to the growth and progression of many human malignancies including colorectal cancer (CRC), which therefore has become a promising target for cancer chemotherapy. Hedyotis diffusa Willd (HDW), as a well-known traditional Chinese herbal medicine, has long been used in China for the clinic treatment of various cancers. Recently, we reported that HDW can inhibit colorectal cancer growth in vivo and in vitro via suppression of the STAT3 pathway. In addition, we demonstrated the anti-angiogenic activity of HDW in vitro. To further elucidate the mechanism of the tumoricidal activity of HDW, by using a CRC mouse xenograft model we evaluated the in vivo effect of the ethanol extract of HDW (EEHDW) on tumor angiogenesis, and investigated the underlying molecular mechanisms. We found that EEHDW could significantly reduce intratumoral microvessel density (MVD), indicating its activity of antitumor angiogenesis in vivo. EEHDW suppressed the activation of SHH signaling in CRC xenograft tumors since it significantly decreased the expression of key mediators of SHH pathway. EEHDW treatment inhibited the expression of the critical SHH signaling target gene VEGF-A as well as its specific receptor VEGFR2. Taken together, we propose for the first time that Hedyotis diffusa Willd inhibits colorectal cancer growth in vivo via inhibition of SHH-mediated tumor angiogenesis.

Authors+Show Affiliations

Academy of Integrative Medicine Biomedical Research Center, Fujian University of Traditional Chinese Medicine, Minhou Shangjie, Fuzhou, Fujian 350122, P.R. China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23291612

Citation

Lin, Jiumao, et al. "Hedyotis Diffusa Willd Extract Suppresses Sonic Hedgehog Signaling Leading to the Inhibition of Colorectal Cancer Angiogenesis." International Journal of Oncology, vol. 42, no. 2, 2013, pp. 651-6.
Lin J, Wei L, Shen A, et al. Hedyotis diffusa Willd extract suppresses Sonic hedgehog signaling leading to the inhibition of colorectal cancer angiogenesis. Int J Oncol. 2013;42(2):651-6.
Lin, J., Wei, L., Shen, A., Cai, Q., Xu, W., Li, H., ... Peng, J. (2013). Hedyotis diffusa Willd extract suppresses Sonic hedgehog signaling leading to the inhibition of colorectal cancer angiogenesis. International Journal of Oncology, 42(2), pp. 651-6. doi:10.3892/ijo.2012.1753.
Lin J, et al. Hedyotis Diffusa Willd Extract Suppresses Sonic Hedgehog Signaling Leading to the Inhibition of Colorectal Cancer Angiogenesis. Int J Oncol. 2013;42(2):651-6. PubMed PMID: 23291612.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hedyotis diffusa Willd extract suppresses Sonic hedgehog signaling leading to the inhibition of colorectal cancer angiogenesis. AU - Lin,Jiumao, AU - Wei,Lihui, AU - Shen,Aling, AU - Cai,Qiaoyan, AU - Xu,Wei, AU - Li,Huang, AU - Zhan,Youzhi, AU - Hong,Zhenfeng, AU - Peng,Jun, Y1 - 2012/12/27/ PY - 2012/11/03/received PY - 2012/12/07/accepted PY - 2013/1/8/entrez PY - 2013/1/8/pubmed PY - 2014/1/8/medline SP - 651 EP - 6 JF - International journal of oncology JO - Int. J. Oncol. VL - 42 IS - 2 N2 - Sonic hedgehog (SHH) signaling pathway promotes the process of angiogenesis, contributing to the growth and progression of many human malignancies including colorectal cancer (CRC), which therefore has become a promising target for cancer chemotherapy. Hedyotis diffusa Willd (HDW), as a well-known traditional Chinese herbal medicine, has long been used in China for the clinic treatment of various cancers. Recently, we reported that HDW can inhibit colorectal cancer growth in vivo and in vitro via suppression of the STAT3 pathway. In addition, we demonstrated the anti-angiogenic activity of HDW in vitro. To further elucidate the mechanism of the tumoricidal activity of HDW, by using a CRC mouse xenograft model we evaluated the in vivo effect of the ethanol extract of HDW (EEHDW) on tumor angiogenesis, and investigated the underlying molecular mechanisms. We found that EEHDW could significantly reduce intratumoral microvessel density (MVD), indicating its activity of antitumor angiogenesis in vivo. EEHDW suppressed the activation of SHH signaling in CRC xenograft tumors since it significantly decreased the expression of key mediators of SHH pathway. EEHDW treatment inhibited the expression of the critical SHH signaling target gene VEGF-A as well as its specific receptor VEGFR2. Taken together, we propose for the first time that Hedyotis diffusa Willd inhibits colorectal cancer growth in vivo via inhibition of SHH-mediated tumor angiogenesis. SN - 1791-2423 UR - https://www.unboundmedicine.com/medline/citation/23291612/Hedyotis_diffusa_Willd_extract_suppresses_Sonic_hedgehog_signaling_leading_to_the_inhibition_of_colorectal_cancer_angiogenesis_ L2 - http://www.spandidos-publications.com/ijo/42/2/651 DB - PRIME DP - Unbound Medicine ER -