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Enzymatic improvement in the polyphenol extractability and antioxidant activity of green tea extracts.
Biosci Biotechnol Biochem. 2013; 77(1):22-9.BB

Abstract

This study describes increases in extraction efficiency and the bioconversion of catechins after treatment with several commercial enzymes. Tannase was also used to improve the anti-radical activities of green tea extracts. Enzymatic treatment with various commercial enzymes was introduced to improve the extraction efficiency of polyphenols. The total polyphenol, flavonoid, and catechin contents and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity of the green tea extract treated with Viscozyme (VG) were significantly higher than those treated with other commercial enzymatic extractions (p<0.05). More than 95% of the epigallocatechingallate (EGCG) and of the epicatechingallate (ECG) was hydrolyzed to epigallocatechin (EGC) and to epicatechin (EC) in successive 20 min treatments with Viscozyme and tannase (TG). Due to its hydrolytic activity, treatment involving tannase resulted in a significant release of gallic acid (GA), EGC, and EC, leading to greater radical scavenging activities. Regarding the IC(50) values of the DPPH and 2,2-azino-di-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radicals, the green tea extract treated with TG showed values of 131.23 and 28.83 µg/mL, VG showed values of 224.70 and 32.54 µg/mL, and normal green tea extract (NG) showed values of 241.11 and 66.27 µg/mL, respectively. These results indicate that successive treatment with Viscozyme and tannase improves the extraction efficiency of polyphenols and increases radical scavenging activities.

Authors+Show Affiliations

Research Institute of Health and Sciences and Department of Food and Nutrition, Korea University, Seoul, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23291774

Citation

Hong, Yang-Hee, et al. "Enzymatic Improvement in the Polyphenol Extractability and Antioxidant Activity of Green Tea Extracts." Bioscience, Biotechnology, and Biochemistry, vol. 77, no. 1, 2013, pp. 22-9.
Hong YH, Jung EY, Park Y, et al. Enzymatic improvement in the polyphenol extractability and antioxidant activity of green tea extracts. Biosci Biotechnol Biochem. 2013;77(1):22-9.
Hong, Y. H., Jung, E. Y., Park, Y., Shin, K. S., Kim, T. Y., Yu, K. W., Chang, U. J., & Suh, H. J. (2013). Enzymatic improvement in the polyphenol extractability and antioxidant activity of green tea extracts. Bioscience, Biotechnology, and Biochemistry, 77(1), 22-9.
Hong YH, et al. Enzymatic Improvement in the Polyphenol Extractability and Antioxidant Activity of Green Tea Extracts. Biosci Biotechnol Biochem. 2013;77(1):22-9. PubMed PMID: 23291774.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enzymatic improvement in the polyphenol extractability and antioxidant activity of green tea extracts. AU - Hong,Yang-Hee, AU - Jung,Eun Young, AU - Park,Yooheon, AU - Shin,Kwang-Soon, AU - Kim,Tae Young, AU - Yu,Kwang-Won, AU - Chang,Un Jae, AU - Suh,Hyung Joo, Y1 - 2013/01/07/ PY - 2013/1/8/entrez PY - 2013/1/8/pubmed PY - 2013/7/6/medline SP - 22 EP - 9 JF - Bioscience, biotechnology, and biochemistry JO - Biosci Biotechnol Biochem VL - 77 IS - 1 N2 - This study describes increases in extraction efficiency and the bioconversion of catechins after treatment with several commercial enzymes. Tannase was also used to improve the anti-radical activities of green tea extracts. Enzymatic treatment with various commercial enzymes was introduced to improve the extraction efficiency of polyphenols. The total polyphenol, flavonoid, and catechin contents and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity of the green tea extract treated with Viscozyme (VG) were significantly higher than those treated with other commercial enzymatic extractions (p<0.05). More than 95% of the epigallocatechingallate (EGCG) and of the epicatechingallate (ECG) was hydrolyzed to epigallocatechin (EGC) and to epicatechin (EC) in successive 20 min treatments with Viscozyme and tannase (TG). Due to its hydrolytic activity, treatment involving tannase resulted in a significant release of gallic acid (GA), EGC, and EC, leading to greater radical scavenging activities. Regarding the IC(50) values of the DPPH and 2,2-azino-di-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radicals, the green tea extract treated with TG showed values of 131.23 and 28.83 µg/mL, VG showed values of 224.70 and 32.54 µg/mL, and normal green tea extract (NG) showed values of 241.11 and 66.27 µg/mL, respectively. These results indicate that successive treatment with Viscozyme and tannase improves the extraction efficiency of polyphenols and increases radical scavenging activities. SN - 1347-6947 UR - https://www.unboundmedicine.com/medline/citation/23291774/Enzymatic_improvement_in_the_polyphenol_extractability_and_antioxidant_activity_of_green_tea_extracts_ L2 - https://joi.jlc.jst.go.jp/DN/JST.JSTAGE/bbb/120373?lang=en&amp;from=PubMed DB - PRIME DP - Unbound Medicine ER -