Tags

Type your tag names separated by a space and hit enter

The ClC-3 chloride channel and osmoregulation in the European sea bass, Dicentrarchus labrax.
J Comp Physiol B 2013; 183(5):641-62JC

Abstract

Dicentrarchus labrax migrates between sea (SW), brackish and fresh water (FW) where chloride concentrations and requirements for chloride handling change: in FW, fish absorb chloride and restrict renal losses; in SW, they excrete chloride. In this study, the expression and localization of ClC-3 and Na(+)/K(+)-ATPase (NKA) were studied in fish adapted to SW, or exposed to FW from 10 min to 30 days. In gills, NKA-α1 subunit expression transiently increased from 10 min and reached a stabilized intermediate expression level after 24 h in FW. ClC-3 co-localized with NKA in the basolateral membrane of mitochondria-rich cells (MRCs) at all conditions. The intensity of MRC ClC-3 immunostaining was significantly higher (by 50 %) 1 h after the transfer to FW, whereas the branchial ClC-3 protein expression was 30 % higher 7 days after the transfer as compared to SW. This is consistent with the increased number of immunopositive MRCs (immunostained for NKA and ClC-3). However, the ClC-3 mRNA expression was significantly lower in FW gills. In the kidney, after FW transfer, a transient decrease in NKA-α1 subunit expression was followed by significantly higher stable levels from 24 h. The low ClC-3 protein expression detected at both salinities was not observed by immunocytochemistry in the SW kidney; ClC-3 was localized in the basal membrane of the collecting ducts and tubules 7 and 30 days after transfer to FW. Renal ClC-3 mRNA expression, however, seemed higher in SW than in FW. The potential role of this chloride channel ClC-3 in osmoregulatory and osmosensing mechanisms is discussed.

Authors+Show Affiliations

Equipe Adaptation Ecophysiologique et Ontogenèse, UMR5119 - EcoSyM, UM2-UM1-CNRS-IRD-IFREMER, cc 092, Place E. Bataillon, 34095 Montpellier cedex 05, France. bossusmaryline@hotmail.frNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23292336

Citation

Bossus, Maryline, et al. "The ClC-3 Chloride Channel and Osmoregulation in the European Sea Bass, Dicentrarchus Labrax." Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology, vol. 183, no. 5, 2013, pp. 641-62.
Bossus M, Charmantier G, Blondeau-Bidet E, et al. The ClC-3 chloride channel and osmoregulation in the European sea bass, Dicentrarchus labrax. J Comp Physiol B, Biochem Syst Environ Physiol. 2013;183(5):641-62.
Bossus, M., Charmantier, G., Blondeau-Bidet, E., Valletta, B., Boulo, V., & Lorin-Nebel, C. (2013). The ClC-3 chloride channel and osmoregulation in the European sea bass, Dicentrarchus labrax. Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology, 183(5), pp. 641-62. doi:10.1007/s00360-012-0737-9.
Bossus M, et al. The ClC-3 Chloride Channel and Osmoregulation in the European Sea Bass, Dicentrarchus Labrax. J Comp Physiol B, Biochem Syst Environ Physiol. 2013;183(5):641-62. PubMed PMID: 23292336.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The ClC-3 chloride channel and osmoregulation in the European sea bass, Dicentrarchus labrax. AU - Bossus,Maryline, AU - Charmantier,Guy, AU - Blondeau-Bidet,Eva, AU - Valletta,Bianca, AU - Boulo,Viviane, AU - Lorin-Nebel,Catherine, Y1 - 2013/01/05/ PY - 2012/06/26/received PY - 2012/11/23/accepted PY - 2012/09/27/revised PY - 2013/1/8/entrez PY - 2013/1/8/pubmed PY - 2013/10/23/medline SP - 641 EP - 62 JF - Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology JO - J. Comp. Physiol. B, Biochem. Syst. Environ. Physiol. VL - 183 IS - 5 N2 - Dicentrarchus labrax migrates between sea (SW), brackish and fresh water (FW) where chloride concentrations and requirements for chloride handling change: in FW, fish absorb chloride and restrict renal losses; in SW, they excrete chloride. In this study, the expression and localization of ClC-3 and Na(+)/K(+)-ATPase (NKA) were studied in fish adapted to SW, or exposed to FW from 10 min to 30 days. In gills, NKA-α1 subunit expression transiently increased from 10 min and reached a stabilized intermediate expression level after 24 h in FW. ClC-3 co-localized with NKA in the basolateral membrane of mitochondria-rich cells (MRCs) at all conditions. The intensity of MRC ClC-3 immunostaining was significantly higher (by 50 %) 1 h after the transfer to FW, whereas the branchial ClC-3 protein expression was 30 % higher 7 days after the transfer as compared to SW. This is consistent with the increased number of immunopositive MRCs (immunostained for NKA and ClC-3). However, the ClC-3 mRNA expression was significantly lower in FW gills. In the kidney, after FW transfer, a transient decrease in NKA-α1 subunit expression was followed by significantly higher stable levels from 24 h. The low ClC-3 protein expression detected at both salinities was not observed by immunocytochemistry in the SW kidney; ClC-3 was localized in the basal membrane of the collecting ducts and tubules 7 and 30 days after transfer to FW. Renal ClC-3 mRNA expression, however, seemed higher in SW than in FW. The potential role of this chloride channel ClC-3 in osmoregulatory and osmosensing mechanisms is discussed. SN - 1432-136X UR - https://www.unboundmedicine.com/medline/citation/23292336/The_ClC_3_chloride_channel_and_osmoregulation_in_the_European_sea_bass_Dicentrarchus_labrax_ L2 - http://dx.doi.org/10.1007/s00360-012-0737-9 DB - PRIME DP - Unbound Medicine ER -