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Development and validation of RP-HPLC method: scope of application in the determination of oil solubility of paclitaxel.
J Chromatogr Sci. 2014 Jan; 52(1):68-74.JC

Abstract

A simple, reproducible, feasible and innovative reversed-phase high-performance liquid chromatographic method was developed and validated for the quantitative determination of paclitaxel dissolved in various oils. The method was validated after extraction of the analyte from capryol 90, triacetin and olive oil. The method was conducted on a Hypersil BDS C18 column, 250 × 4.6 mm, 5 µm particle size, with a mobile phase composed of acetonitrile: 10 mM KH2PO4 buffer (pH 3.5) (55:45, v/v) and detection at 227 nm. The linearity, in the range of 5 to 50 µg/mL, presented determination coefficients of 0.9983, 0.9997 and 0.9990 in capryol 90, triacetin and olive oil, respectively, calculated by the least-squares regression method. Intra-day precision values for percentages recovered were 0.68 to 0.80, 0.83 to 1.13 and 0.97 to 1.88, and inter day precision values were 1.52 to 1.92, 1.43 to 1.83 and 1.26 to 2.06 for capryol 90, triacetin and olive oil, respectively. The recovery of paclitaxel from the capryol 90, triacetin and olive oil ranged from 97.94 to 103.55, 96.85 to 103.27 and 97.14 to 103.64%, respectively. This developed and validated method was successfully applied to quantitatively assess paclitaxel dissolved in various oils. The solubility of paclitaxel was found to be higher in triacetin than in other tested oils.

Authors+Show Affiliations

1Bioequivalence Study Centre, Department of Pharmaceutical Technology, Jadavpur University, Kolkata-700 032, India.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23293041

Citation

Choudhury, Hira, et al. "Development and Validation of RP-HPLC Method: Scope of Application in the Determination of Oil Solubility of Paclitaxel." Journal of Chromatographic Science, vol. 52, no. 1, 2014, pp. 68-74.
Choudhury H, Gorain B, Karmakar S, et al. Development and validation of RP-HPLC method: scope of application in the determination of oil solubility of paclitaxel. J Chromatogr Sci. 2014;52(1):68-74.
Choudhury, H., Gorain, B., Karmakar, S., & Pal, T. K. (2014). Development and validation of RP-HPLC method: scope of application in the determination of oil solubility of paclitaxel. Journal of Chromatographic Science, 52(1), 68-74. https://doi.org/10.1093/chromsci/bms206
Choudhury H, et al. Development and Validation of RP-HPLC Method: Scope of Application in the Determination of Oil Solubility of Paclitaxel. J Chromatogr Sci. 2014;52(1):68-74. PubMed PMID: 23293041.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development and validation of RP-HPLC method: scope of application in the determination of oil solubility of paclitaxel. AU - Choudhury,Hira, AU - Gorain,Bapi, AU - Karmakar,Sanmoy, AU - Pal,Tapan Kumar, Y1 - 2013/01/04/ PY - 2013/1/8/entrez PY - 2013/1/8/pubmed PY - 2014/7/8/medline SP - 68 EP - 74 JF - Journal of chromatographic science JO - J Chromatogr Sci VL - 52 IS - 1 N2 - A simple, reproducible, feasible and innovative reversed-phase high-performance liquid chromatographic method was developed and validated for the quantitative determination of paclitaxel dissolved in various oils. The method was validated after extraction of the analyte from capryol 90, triacetin and olive oil. The method was conducted on a Hypersil BDS C18 column, 250 × 4.6 mm, 5 µm particle size, with a mobile phase composed of acetonitrile: 10 mM KH2PO4 buffer (pH 3.5) (55:45, v/v) and detection at 227 nm. The linearity, in the range of 5 to 50 µg/mL, presented determination coefficients of 0.9983, 0.9997 and 0.9990 in capryol 90, triacetin and olive oil, respectively, calculated by the least-squares regression method. Intra-day precision values for percentages recovered were 0.68 to 0.80, 0.83 to 1.13 and 0.97 to 1.88, and inter day precision values were 1.52 to 1.92, 1.43 to 1.83 and 1.26 to 2.06 for capryol 90, triacetin and olive oil, respectively. The recovery of paclitaxel from the capryol 90, triacetin and olive oil ranged from 97.94 to 103.55, 96.85 to 103.27 and 97.14 to 103.64%, respectively. This developed and validated method was successfully applied to quantitatively assess paclitaxel dissolved in various oils. The solubility of paclitaxel was found to be higher in triacetin than in other tested oils. SN - 1945-239X UR - https://www.unboundmedicine.com/medline/citation/23293041/Development_and_validation_of_RP_HPLC_method:_scope_of_application_in_the_determination_of_oil_solubility_of_paclitaxel_ L2 - https://academic.oup.com/chromsci/article-lookup/doi/10.1093/chromsci/bms206 DB - PRIME DP - Unbound Medicine ER -