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Docosahexaenoic acid (DHA) attenuated paraquat induced lung damage in mice.
Inhal Toxicol. 2013 Jan; 25(1):9-16.IT

Abstract

CONTEXT

Accumulating evidences have proposed the critical roles of oxidative stress in the etiology of lung injury caused by paraquat (PQ). Docosahexaenoic acid (DHA) is an essential n-3 polyunsaturated fatty acid (PUFA), which has been proved to possess prominent antioxidative and anti-inflammatory effects.

OBJECTIVE

The aim of this study was to evaluate effects of DHA against acute lung injury (ALI) induced by PQ in mice.

MATERIALS AND METHODS

Male Kunming mice were randomly divided into three groups: control group, PQ group, and PQ+DHA group (n = 24). The mice of PQ+DHA group received 500 mg/kg bodyweight DHA by gavage daily for consecutive 14 days. On day 8, the mice in PQ and PQ+DHA groups received a single oral dose of 50 mg/kg bodyweight PQ. All the mice were sacrificed on day 15. The myeloperoxidase (MPO) activities, levels of the malondialdehyde (MDA) and glutathione (GSH), and the 4-hydroxynonenal (4-HNE) and MDA modified proteins of lung were investigated.

RESULTS

DHA treatment significantly increased the survival rate of mice treated with PQ. Pulmonary MPO activities and MDA contents were elevated in the mice of the PQ group, while the GSH level was reduced. Furthermore, levels of 4-HNE and MDA modified protein in lungs of the PQ group mice were significantly increased. All the above changes were significantly inhibited by DHA pretreatment. Morphological examination revealed that DHA effectively attenuated the hyperemia, edema of ALI induced by PQ.

CONCLUSION

These results demonstrated that DHA could effectively attenuate PQ-induced ALI in mice probably via its antioxidant activity.

Authors+Show Affiliations

Institute of Toxicology, School of Public Health, Shandong University, Jinan, Shandong Province 250012, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23293968

Citation

Chen, Jingjing, et al. "Docosahexaenoic Acid (DHA) Attenuated Paraquat Induced Lung Damage in Mice." Inhalation Toxicology, vol. 25, no. 1, 2013, pp. 9-16.
Chen J, Zeng T, Bi Y, et al. Docosahexaenoic acid (DHA) attenuated paraquat induced lung damage in mice. Inhal Toxicol. 2013;25(1):9-16.
Chen, J., Zeng, T., Bi, Y., Zhong, Z., Xie, K., & Zhao, X. (2013). Docosahexaenoic acid (DHA) attenuated paraquat induced lung damage in mice. Inhalation Toxicology, 25(1), 9-16. https://doi.org/10.3109/08958378.2012.750405
Chen J, et al. Docosahexaenoic Acid (DHA) Attenuated Paraquat Induced Lung Damage in Mice. Inhal Toxicol. 2013;25(1):9-16. PubMed PMID: 23293968.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Docosahexaenoic acid (DHA) attenuated paraquat induced lung damage in mice. AU - Chen,Jingjing, AU - Zeng,Tao, AU - Bi,Ye, AU - Zhong,Zhixia, AU - Xie,Keqin, AU - Zhao,Xiulan, PY - 2013/1/9/entrez PY - 2013/1/9/pubmed PY - 2013/6/12/medline SP - 9 EP - 16 JF - Inhalation toxicology JO - Inhal Toxicol VL - 25 IS - 1 N2 - CONTEXT: Accumulating evidences have proposed the critical roles of oxidative stress in the etiology of lung injury caused by paraquat (PQ). Docosahexaenoic acid (DHA) is an essential n-3 polyunsaturated fatty acid (PUFA), which has been proved to possess prominent antioxidative and anti-inflammatory effects. OBJECTIVE: The aim of this study was to evaluate effects of DHA against acute lung injury (ALI) induced by PQ in mice. MATERIALS AND METHODS: Male Kunming mice were randomly divided into three groups: control group, PQ group, and PQ+DHA group (n = 24). The mice of PQ+DHA group received 500 mg/kg bodyweight DHA by gavage daily for consecutive 14 days. On day 8, the mice in PQ and PQ+DHA groups received a single oral dose of 50 mg/kg bodyweight PQ. All the mice were sacrificed on day 15. The myeloperoxidase (MPO) activities, levels of the malondialdehyde (MDA) and glutathione (GSH), and the 4-hydroxynonenal (4-HNE) and MDA modified proteins of lung were investigated. RESULTS: DHA treatment significantly increased the survival rate of mice treated with PQ. Pulmonary MPO activities and MDA contents were elevated in the mice of the PQ group, while the GSH level was reduced. Furthermore, levels of 4-HNE and MDA modified protein in lungs of the PQ group mice were significantly increased. All the above changes were significantly inhibited by DHA pretreatment. Morphological examination revealed that DHA effectively attenuated the hyperemia, edema of ALI induced by PQ. CONCLUSION: These results demonstrated that DHA could effectively attenuate PQ-induced ALI in mice probably via its antioxidant activity. SN - 1091-7691 UR - https://www.unboundmedicine.com/medline/citation/23293968/Docosahexaenoic_acid__DHA__attenuated_paraquat_induced_lung_damage_in_mice_ L2 - https://www.tandfonline.com/doi/full/10.3109/08958378.2012.750405 DB - PRIME DP - Unbound Medicine ER -