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A non-electrolyte haemolysis assay for diagnosis and prognosis of sickle cell disease.
J Physiol. 2013 Mar 15; 591(6):1463-74.JP

Abstract

Abstract Red blood cells (RBCs) from patients with sickle cell disease (SCD) lyse in deoxygenated isosmotic non-electrolyte solutions. Haemolysis has features which suggest that it is linked to activation of the pathway termed Psickle. This pathway is usually described as a non-specific cationic conductance activated by deoxygenation, HbS polymerisation and RBC sickling. The current work addresses the hypothesis that this haemolysis will provide a novel diagnostic and prognostic test for SCD, dependent on the altered properties of the RBC membrane resulting from HbS polymerisation. A simple test represented by this haemolysis assay would be useful especially in less affluent deprived areas of the world where SCD is most prevalent. RBCs from HbSS and most HbSC individuals showed progressive lysis in deoxygenated isosmotic sucrose solution at pH 7.4 to a level greater than that observed with RBCs from HbAS or HbAA individuals. Cytochalasin B prevented haemolysis. Haemolysis was temperature- and pH-dependent. It required near physiological temperatures to occur in deoxygenated sucrose solutions at pH 7.4. At pH 6, haemolysis occurred even in oxygenated samples. Haemolysis was reduced in patients on long-term (>5 months) hydroxyurea treatment. Several manoeuvres which stabilise soluble HbS (aromatic aldehydes o-vanillin or 5-hydroxymethyl, and urea) reduced haemolysis, an effect not due to increased oxygen affinity. Conditions designed to elicit HbS polymerisation in cells from sickle trait patients (deoxygenated hyperosmotic sucrose solutions at pH 6) supported their haemolysis. These findings are consistent with haemolysis requiring HbS polymerisation and support the hypothesis that this may be used as a test for SCD.

Authors+Show Affiliations

Department of Veterinary Medicine, Madingley Road, Cambridge CB3 0ES, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23297308

Citation

Milligan, C, et al. "A Non-electrolyte Haemolysis Assay for Diagnosis and Prognosis of Sickle Cell Disease." The Journal of Physiology, vol. 591, no. 6, 2013, pp. 1463-74.
Milligan C, Rees DC, Ellory JC, et al. A non-electrolyte haemolysis assay for diagnosis and prognosis of sickle cell disease. J Physiol. 2013;591(6):1463-74.
Milligan, C., Rees, D. C., Ellory, J. C., Osei, A., Browning, J. A., Hannemann, A., & Gibson, J. S. (2013). A non-electrolyte haemolysis assay for diagnosis and prognosis of sickle cell disease. The Journal of Physiology, 591(6), 1463-74. https://doi.org/10.1113/jphysiol.2012.246579
Milligan C, et al. A Non-electrolyte Haemolysis Assay for Diagnosis and Prognosis of Sickle Cell Disease. J Physiol. 2013 Mar 15;591(6):1463-74. PubMed PMID: 23297308.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A non-electrolyte haemolysis assay for diagnosis and prognosis of sickle cell disease. AU - Milligan,C, AU - Rees,D C, AU - Ellory,J C, AU - Osei,A, AU - Browning,J A, AU - Hannemann,A, AU - Gibson,J S, Y1 - 2013/01/07/ PY - 2013/1/9/entrez PY - 2013/1/9/pubmed PY - 2013/8/31/medline SP - 1463 EP - 74 JF - The Journal of physiology JO - J Physiol VL - 591 IS - 6 N2 - Abstract Red blood cells (RBCs) from patients with sickle cell disease (SCD) lyse in deoxygenated isosmotic non-electrolyte solutions. Haemolysis has features which suggest that it is linked to activation of the pathway termed Psickle. This pathway is usually described as a non-specific cationic conductance activated by deoxygenation, HbS polymerisation and RBC sickling. The current work addresses the hypothesis that this haemolysis will provide a novel diagnostic and prognostic test for SCD, dependent on the altered properties of the RBC membrane resulting from HbS polymerisation. A simple test represented by this haemolysis assay would be useful especially in less affluent deprived areas of the world where SCD is most prevalent. RBCs from HbSS and most HbSC individuals showed progressive lysis in deoxygenated isosmotic sucrose solution at pH 7.4 to a level greater than that observed with RBCs from HbAS or HbAA individuals. Cytochalasin B prevented haemolysis. Haemolysis was temperature- and pH-dependent. It required near physiological temperatures to occur in deoxygenated sucrose solutions at pH 7.4. At pH 6, haemolysis occurred even in oxygenated samples. Haemolysis was reduced in patients on long-term (>5 months) hydroxyurea treatment. Several manoeuvres which stabilise soluble HbS (aromatic aldehydes o-vanillin or 5-hydroxymethyl, and urea) reduced haemolysis, an effect not due to increased oxygen affinity. Conditions designed to elicit HbS polymerisation in cells from sickle trait patients (deoxygenated hyperosmotic sucrose solutions at pH 6) supported their haemolysis. These findings are consistent with haemolysis requiring HbS polymerisation and support the hypothesis that this may be used as a test for SCD. SN - 1469-7793 UR - https://www.unboundmedicine.com/medline/citation/23297308/A_non_electrolyte_haemolysis_assay_for_diagnosis_and_prognosis_of_sickle_cell_disease_ L2 - https://doi.org/10.1113/jphysiol.2012.246579 DB - PRIME DP - Unbound Medicine ER -