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Geniposidic acid protects against D-galactosamine and lipopolysaccharide-induced hepatic failure in mice.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Geniposidic acid (GA) is an iridoid glucoside isolated from Gardeniae jasminoides Ellis (Rubiaceae) that has long been used to treat inflammation, jaundice and hepatic disorders.

AIMS OF THE STUDY

This study examined the cytoprotective properties of GA against D-galactosamine (GalN)/lipopolysaccharide (LPS)-induced fulminant hepatic failure.

MATERIALS AND METHODS

Mice were given an intraperitoneal injection of GA (12.5, 25, 50 mg/kg) 1h before receiving GalN (800 mg/kg)/LPS (40 μg/kg). Liver and blood samples were collected 1 and 8 h after GalN/LPS injection.

RESULTS

The survival rate of the GA group was significantly higher than the control. GalN/LPS increased serum aminotransferase activity, serum tumor necrosis factor-α level and hepatic lipid peroxidation and decreased hepatic glutathione content. These changes were attenuated by GA. GA augmented increases in serum interleukin-6 level, heme oxygenase-1 and NF-E2-related factor 2 protein expression. Mice treated with GA decreased cleaved caspase-8 and caspase-3 protein expression and showed significantly fewer apoptotic cells. GA increased Bcl-xL protein expression and decreased Bax protein expression. Moreover, GA treatment enhanced phosphorylation of signal transducer and activator of transcription 3.

CONCLUSION

Our findings suggest that geniposidic acid alleviates GalN/LPS-induced liver injury by enhancing antioxidative defense system and reducing apoptotic signaling pathways.

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  • Authors+Show Affiliations

    ,

    School of Pharmacy, Sungkyunkwan University, Suwon, 440-746, Republic of Korea.

    , , , ,

    Source

    Journal of ethnopharmacology 146:1 2013 Mar 07 pg 271-7

    MeSH

    Animals
    Apoptosis
    Fruit
    Galactosamine
    Gardenia
    Glutathione
    Heme Oxygenase-1
    Interleukin-6
    Iridoid Glucosides
    Lipid Peroxidation
    Lipopolysaccharides
    Liver Failure
    Male
    Membrane Proteins
    Mice
    Mice, Inbred ICR
    NF-E2-Related Factor 2
    Phytotherapy
    Plant Extracts
    Protective Agents
    STAT3 Transcription Factor
    Tumor Necrosis Factor-alpha
    bcl-2-Associated X Protein
    bcl-X Protein

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    23298456

    Citation

    Kim, So-Jin, et al. "Geniposidic Acid Protects Against D-galactosamine and Lipopolysaccharide-induced Hepatic Failure in Mice." Journal of Ethnopharmacology, vol. 146, no. 1, 2013, pp. 271-7.
    Kim SJ, Kim KM, Park J, et al. Geniposidic acid protects against D-galactosamine and lipopolysaccharide-induced hepatic failure in mice. J Ethnopharmacol. 2013;146(1):271-7.
    Kim, S. J., Kim, K. M., Park, J., Kwak, J. H., Kim, Y. S., & Lee, S. M. (2013). Geniposidic acid protects against D-galactosamine and lipopolysaccharide-induced hepatic failure in mice. Journal of Ethnopharmacology, 146(1), pp. 271-7. doi:10.1016/j.jep.2012.12.042.
    Kim SJ, et al. Geniposidic Acid Protects Against D-galactosamine and Lipopolysaccharide-induced Hepatic Failure in Mice. J Ethnopharmacol. 2013 Mar 7;146(1):271-7. PubMed PMID: 23298456.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Geniposidic acid protects against D-galactosamine and lipopolysaccharide-induced hepatic failure in mice. AU - Kim,So-Jin, AU - Kim,Kang-Min, AU - Park,Juhyun, AU - Kwak,Jong-Hwan, AU - Kim,Yeong Shik, AU - Lee,Sun-Mee, Y1 - 2013/01/05/ PY - 2012/06/24/received PY - 2012/10/23/revised PY - 2012/12/14/accepted PY - 2013/1/10/entrez PY - 2013/1/10/pubmed PY - 2013/8/14/medline SP - 271 EP - 7 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 146 IS - 1 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Geniposidic acid (GA) is an iridoid glucoside isolated from Gardeniae jasminoides Ellis (Rubiaceae) that has long been used to treat inflammation, jaundice and hepatic disorders. AIMS OF THE STUDY: This study examined the cytoprotective properties of GA against D-galactosamine (GalN)/lipopolysaccharide (LPS)-induced fulminant hepatic failure. MATERIALS AND METHODS: Mice were given an intraperitoneal injection of GA (12.5, 25, 50 mg/kg) 1h before receiving GalN (800 mg/kg)/LPS (40 μg/kg). Liver and blood samples were collected 1 and 8 h after GalN/LPS injection. RESULTS: The survival rate of the GA group was significantly higher than the control. GalN/LPS increased serum aminotransferase activity, serum tumor necrosis factor-α level and hepatic lipid peroxidation and decreased hepatic glutathione content. These changes were attenuated by GA. GA augmented increases in serum interleukin-6 level, heme oxygenase-1 and NF-E2-related factor 2 protein expression. Mice treated with GA decreased cleaved caspase-8 and caspase-3 protein expression and showed significantly fewer apoptotic cells. GA increased Bcl-xL protein expression and decreased Bax protein expression. Moreover, GA treatment enhanced phosphorylation of signal transducer and activator of transcription 3. CONCLUSION: Our findings suggest that geniposidic acid alleviates GalN/LPS-induced liver injury by enhancing antioxidative defense system and reducing apoptotic signaling pathways. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/23298456/Geniposidic_acid_protects_against_D_galactosamine_and_lipopolysaccharide_induced_hepatic_failure_in_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(12)00892-6 DB - PRIME DP - Unbound Medicine ER -