Tags

Type your tag names separated by a space and hit enter

A novel homogeneous label-free aptasensor for 2,4,6-trinitrotoluene detection based on an assembly strategy of electrochemiluminescent graphene oxide with gold nanoparticles and aptamer.
Biosens Bioelectron. 2013 May 15; 43:137-42.BB

Abstract

We report a novel homogeneous label-free aptasensor for 2,4,6-trinitrotoluene (TNT) detection based on an assembly strategy of electrochemiluminescent graphene oxide (GO) with gold nanoparticles (AuNPs) and aptamer. In this sensing strategy, the anti-TNT aptamer was first assembled with AuNPs to form aptamer-AuNPs. Then ruthenium(II) complex functionalized GO (denoted as Ru-GO) was assembled with aptamer-AuNPs by electrostatic interaction. AuNPs could directly quench the electrochemiluminescence (ECL) emission of ruthenium(II) complex on the surface of Ru-GO due to the energy transfer from luminophore to the AuNPs. Weak ECL signal of the assembly was obtained. In the presence of target molecule TNT, the aptamer-AuNPs would aggregate partly due to the aptamer-target interaction and reduce quenching effect, leading to ECL signal restoration and strong ECL signal was obtained. TNT in a range of 0.01-100ngmL(-1) could be detected by use of the ECL intensity discrepancy with a low detection limit of 3.6pgmL(-1). The aptasensor also showed high selectivity towards TNT against 2,4-dinitrotoluene, p-nitrotoluene and nitrobenzene. The present aptasensor has been successfully applied to the detection of TNT in real water samples. Compared with previous reported sensors, this homogenous aptasensor avoided complicated labeling and purification procedure and showed magnificent sensitivity and high selectivity, which made it not only convenient but also time-saving and applicable. Furthermore, this sensing strategy also provides a promising way to develop new ECL aptasensor for other analytes by virtue of other aptamers.

Authors+Show Affiliations

CAS Key Laboratory of Soft Matter Chemistry, Department of Chemistry, University of Science and Technology of China, 96 Jinzhai Road, Hefei, Anhui 230026, PR China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23298624

Citation

Yu, Yuqi, et al. "A Novel Homogeneous Label-free Aptasensor for 2,4,6-trinitrotoluene Detection Based On an Assembly Strategy of Electrochemiluminescent Graphene Oxide With Gold Nanoparticles and Aptamer." Biosensors & Bioelectronics, vol. 43, 2013, pp. 137-42.
Yu Y, Cao Q, Zhou M, et al. A novel homogeneous label-free aptasensor for 2,4,6-trinitrotoluene detection based on an assembly strategy of electrochemiluminescent graphene oxide with gold nanoparticles and aptamer. Biosens Bioelectron. 2013;43:137-42.
Yu, Y., Cao, Q., Zhou, M., & Cui, H. (2013). A novel homogeneous label-free aptasensor for 2,4,6-trinitrotoluene detection based on an assembly strategy of electrochemiluminescent graphene oxide with gold nanoparticles and aptamer. Biosensors & Bioelectronics, 43, 137-42. https://doi.org/10.1016/j.bios.2012.12.018
Yu Y, et al. A Novel Homogeneous Label-free Aptasensor for 2,4,6-trinitrotoluene Detection Based On an Assembly Strategy of Electrochemiluminescent Graphene Oxide With Gold Nanoparticles and Aptamer. Biosens Bioelectron. 2013 May 15;43:137-42. PubMed PMID: 23298624.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A novel homogeneous label-free aptasensor for 2,4,6-trinitrotoluene detection based on an assembly strategy of electrochemiluminescent graphene oxide with gold nanoparticles and aptamer. AU - Yu,Yuqi, AU - Cao,Qiao, AU - Zhou,Ming, AU - Cui,Hua, Y1 - 2012/12/14/ PY - 2012/10/11/received PY - 2012/11/23/revised PY - 2012/12/09/accepted PY - 2013/1/10/entrez PY - 2013/1/10/pubmed PY - 2013/8/22/medline SP - 137 EP - 42 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 43 N2 - We report a novel homogeneous label-free aptasensor for 2,4,6-trinitrotoluene (TNT) detection based on an assembly strategy of electrochemiluminescent graphene oxide (GO) with gold nanoparticles (AuNPs) and aptamer. In this sensing strategy, the anti-TNT aptamer was first assembled with AuNPs to form aptamer-AuNPs. Then ruthenium(II) complex functionalized GO (denoted as Ru-GO) was assembled with aptamer-AuNPs by electrostatic interaction. AuNPs could directly quench the electrochemiluminescence (ECL) emission of ruthenium(II) complex on the surface of Ru-GO due to the energy transfer from luminophore to the AuNPs. Weak ECL signal of the assembly was obtained. In the presence of target molecule TNT, the aptamer-AuNPs would aggregate partly due to the aptamer-target interaction and reduce quenching effect, leading to ECL signal restoration and strong ECL signal was obtained. TNT in a range of 0.01-100ngmL(-1) could be detected by use of the ECL intensity discrepancy with a low detection limit of 3.6pgmL(-1). The aptasensor also showed high selectivity towards TNT against 2,4-dinitrotoluene, p-nitrotoluene and nitrobenzene. The present aptasensor has been successfully applied to the detection of TNT in real water samples. Compared with previous reported sensors, this homogenous aptasensor avoided complicated labeling and purification procedure and showed magnificent sensitivity and high selectivity, which made it not only convenient but also time-saving and applicable. Furthermore, this sensing strategy also provides a promising way to develop new ECL aptasensor for other analytes by virtue of other aptamers. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/23298624/A_novel_homogeneous_label_free_aptasensor_for_246_trinitrotoluene_detection_based_on_an_assembly_strategy_of_electrochemiluminescent_graphene_oxide_with_gold_nanoparticles_and_aptamer_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(12)00874-3 DB - PRIME DP - Unbound Medicine ER -