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Contribution of T-cell receptor gamma gene rearrangement by polymerase chain reaction and immunohistochemistry to the histological diagnosis of early mycosis fungoides.
Saudi Med J 2013; 34(1):19-23SM

Abstract

OBJECTIVE

To study the role of immuno-histochemistry and T-cell receptor (TCR) gamma gene rearrangement analysis in the diagnosis of mycosis fungoides (MF).

METHODS

The study design was retrospective, and 73 cases were selected from the archive of the Pathology Department, School of Medicine, Cukurova University, Adana, Turkey, between January 2004 and December 2009. Thirty-nine MF cases with classical histomorphology, 16 cases with suspicious histomorphology for MF as the inconclusive group, and 18 cases with benign inflammatory dermatoses as the control group were involved in the study. The slides were evaluated for the presence or absence of the histopathological criteria for MF. Immunohistochemically, CD3, CD4, and CD8 were performed in all cases, and their counts and CD4/CD8 ratio were noted. Presence clonal TCR gamma gene rearrangement was evaluated by polymerase chain reaction (PCR).

RESULTS

The histopathological parameters suggestive of MF were epidermotropism (p=0.000), presence of Pautrier microabscess (p=0.004), and atypical lymphocyte (p=0.000). Immunohistochemically, CD4 percentage (p=0.006) and CD4/CD8 ratio (p=0.010) were statistically significant parameters with univariate analysis. Clonality was present in 76.9% of MF cases and 37.5% in the inconclusive group. Four of 6 clonality positive cases in the inconclusive group were diagnosed as MF in rebiopsies. The CD8 percentage was not statistically different between the 3 groups.

CONCLUSION

In suspicious cases, CD4/CD8 ratio and TCR gamma gene rearrangement can support histopathology in early MF.

Authors+Show Affiliations

Department of Pathology, Cukurova University, Adana, Turkey. arbilavci@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23299154

Citation

Acikalin, Arbil, et al. "Contribution of T-cell Receptor Gamma Gene Rearrangement By Polymerase Chain Reaction and Immunohistochemistry to the Histological Diagnosis of Early Mycosis Fungoides." Saudi Medical Journal, vol. 34, no. 1, 2013, pp. 19-23.
Acikalin A, Bagir E, Tuncer I, et al. Contribution of T-cell receptor gamma gene rearrangement by polymerase chain reaction and immunohistochemistry to the histological diagnosis of early mycosis fungoides. Saudi Med J. 2013;34(1):19-23.
Acikalin, A., Bagir, E., Tuncer, I., Ergin, M., & Denli, Y. (2013). Contribution of T-cell receptor gamma gene rearrangement by polymerase chain reaction and immunohistochemistry to the histological diagnosis of early mycosis fungoides. Saudi Medical Journal, 34(1), pp. 19-23.
Acikalin A, et al. Contribution of T-cell Receptor Gamma Gene Rearrangement By Polymerase Chain Reaction and Immunohistochemistry to the Histological Diagnosis of Early Mycosis Fungoides. Saudi Med J. 2013;34(1):19-23. PubMed PMID: 23299154.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Contribution of T-cell receptor gamma gene rearrangement by polymerase chain reaction and immunohistochemistry to the histological diagnosis of early mycosis fungoides. AU - Acikalin,Arbil, AU - Bagir,Emine, AU - Tuncer,Ilhan, AU - Ergin,Melek, AU - Denli,Yasargul, PY - 2013/1/10/entrez PY - 2013/1/10/pubmed PY - 2013/11/19/medline SP - 19 EP - 23 JF - Saudi medical journal JO - Saudi Med J VL - 34 IS - 1 N2 - OBJECTIVE: To study the role of immuno-histochemistry and T-cell receptor (TCR) gamma gene rearrangement analysis in the diagnosis of mycosis fungoides (MF). METHODS: The study design was retrospective, and 73 cases were selected from the archive of the Pathology Department, School of Medicine, Cukurova University, Adana, Turkey, between January 2004 and December 2009. Thirty-nine MF cases with classical histomorphology, 16 cases with suspicious histomorphology for MF as the inconclusive group, and 18 cases with benign inflammatory dermatoses as the control group were involved in the study. The slides were evaluated for the presence or absence of the histopathological criteria for MF. Immunohistochemically, CD3, CD4, and CD8 were performed in all cases, and their counts and CD4/CD8 ratio were noted. Presence clonal TCR gamma gene rearrangement was evaluated by polymerase chain reaction (PCR). RESULTS: The histopathological parameters suggestive of MF were epidermotropism (p=0.000), presence of Pautrier microabscess (p=0.004), and atypical lymphocyte (p=0.000). Immunohistochemically, CD4 percentage (p=0.006) and CD4/CD8 ratio (p=0.010) were statistically significant parameters with univariate analysis. Clonality was present in 76.9% of MF cases and 37.5% in the inconclusive group. Four of 6 clonality positive cases in the inconclusive group were diagnosed as MF in rebiopsies. The CD8 percentage was not statistically different between the 3 groups. CONCLUSION: In suspicious cases, CD4/CD8 ratio and TCR gamma gene rearrangement can support histopathology in early MF. SN - 0379-5284 UR - https://www.unboundmedicine.com/medline/citation/23299154/Contribution_of_T-cell_receptor_gamma_gene_rearrangement_by_polymerase_chain_reaction_and_immunohistochemistry_to_the_histological_diagnosis_of_early_mycosis_fungoides L2 - http://www.diseaseinfosearch.org/result/5014 DB - PRIME DP - Unbound Medicine ER -