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Optimization of the cutoff value for a commercial anti-dengue virus IgG immunoassay.
Clin Vaccine Immunol. 2013 Mar; 20(3):358-62.CV

Abstract

A commercial anti-dengue virus (anti-DENV) indirect IgG enzyme-linked immunosorbent assay (ELISA) for serological diagnosis was evaluated for its utility in determining previous DENV exposure in U.S. travelers. The Boston Area Travel Medicine Network clinics used Focus Diagnostics anti-DENV IgG ELISA to measure anti-DENV IgG antibodies in 591 pretravel specimens from U.S. residents who had traveled to countries where dengue is endemic. When using the manufacturer's index cutoff value for this ELISA, false-positive results were observed that overestimated the perceived past DENV exposure in U.S. travelers. Validation of 121 of these anti-DENV IgG results by plaque reduction neutralization test (PRNT) was used for receiver operating characteristic (ROC) curve optimization of the index cutoff value from 1 to 3.0, improving the specificity of the anti-DENV IgG ELISA from 24% to 95.7%. Additionally, previous vaccination with yellow fever virus contributed to 52.8% of the false-positive rate in the anti-DENV IgG ELISA results. Optimization of the cutoff value of the anti-DENV IgG ELISA provided better interpretation and confidence in the results and eliminated the need for confirmation by PRNT. The travel history of U.S. travelers was also useful for categorizing these travelers into groups for analysis of previous DENV exposure.

Authors+Show Affiliations

Dengue Branch, Division of Vector-Borne Diseases, Centers For Disease Control and Prevention, San Juan, Puerto Rico.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Validation Study

Language

eng

PubMed ID

23302742

Citation

Marrero-Santos, Karla M., et al. "Optimization of the Cutoff Value for a Commercial Anti-dengue Virus IgG Immunoassay." Clinical and Vaccine Immunology : CVI, vol. 20, no. 3, 2013, pp. 358-62.
Marrero-Santos KM, Beltrán M, Carrión-Lebrón J, et al. Optimization of the cutoff value for a commercial anti-dengue virus IgG immunoassay. Clin Vaccine Immunol. 2013;20(3):358-62.
Marrero-Santos, K. M., Beltrán, M., Carrión-Lebrón, J., Sanchez-Vegas, C., Hamer, D. H., Barnett, E. D., Santiago, L. M., & Hunsperger, E. A. (2013). Optimization of the cutoff value for a commercial anti-dengue virus IgG immunoassay. Clinical and Vaccine Immunology : CVI, 20(3), 358-62. https://doi.org/10.1128/CVI.00429-12
Marrero-Santos KM, et al. Optimization of the Cutoff Value for a Commercial Anti-dengue Virus IgG Immunoassay. Clin Vaccine Immunol. 2013;20(3):358-62. PubMed PMID: 23302742.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Optimization of the cutoff value for a commercial anti-dengue virus IgG immunoassay. AU - Marrero-Santos,Karla M, AU - Beltrán,Manuela, AU - Carrión-Lebrón,Jessica, AU - Sanchez-Vegas,Carolina, AU - Hamer,Davidson H, AU - Barnett,Elizabeth D, AU - Santiago,Luis M, AU - Hunsperger,Elizabeth A, Y1 - 2013/01/09/ PY - 2013/1/11/entrez PY - 2013/1/11/pubmed PY - 2013/8/21/medline SP - 358 EP - 62 JF - Clinical and vaccine immunology : CVI JO - Clin Vaccine Immunol VL - 20 IS - 3 N2 - A commercial anti-dengue virus (anti-DENV) indirect IgG enzyme-linked immunosorbent assay (ELISA) for serological diagnosis was evaluated for its utility in determining previous DENV exposure in U.S. travelers. The Boston Area Travel Medicine Network clinics used Focus Diagnostics anti-DENV IgG ELISA to measure anti-DENV IgG antibodies in 591 pretravel specimens from U.S. residents who had traveled to countries where dengue is endemic. When using the manufacturer's index cutoff value for this ELISA, false-positive results were observed that overestimated the perceived past DENV exposure in U.S. travelers. Validation of 121 of these anti-DENV IgG results by plaque reduction neutralization test (PRNT) was used for receiver operating characteristic (ROC) curve optimization of the index cutoff value from 1 to 3.0, improving the specificity of the anti-DENV IgG ELISA from 24% to 95.7%. Additionally, previous vaccination with yellow fever virus contributed to 52.8% of the false-positive rate in the anti-DENV IgG ELISA results. Optimization of the cutoff value of the anti-DENV IgG ELISA provided better interpretation and confidence in the results and eliminated the need for confirmation by PRNT. The travel history of U.S. travelers was also useful for categorizing these travelers into groups for analysis of previous DENV exposure. SN - 1556-679X UR - https://www.unboundmedicine.com/medline/citation/23302742/Optimization_of_the_cutoff_value_for_a_commercial_anti_dengue_virus_IgG_immunoassay_ L2 - http://cvi.asm.org/cgi/pmidlookup?view=long&pmid=23302742 DB - PRIME DP - Unbound Medicine ER -