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Activation of the renin-angiotensin system by a low-salt diet does not augment intratubular angiotensinogen and angiotensin II in rats.
Am J Physiol Renal Physiol. 2013 Mar 01; 304(5):F505-14.AJ

Abstract

In angiotensin II (ANG II) infusion hypertension, there is an augmentation of intratubular angiotensinogen (AGT) and ANG II leading to increased urinary AGT and ANG II excretion rates associated with tissue injury. However, the changes in urinary AGT and ANG II excretion rates and markers of renal injury during physiologically induced stimulation of the renin-angiotensin system (RAS) by a low-salt diet remain unclear. Male Sprague-Dawley rats received a low-salt diet (0.03% NaCl; n = 6) and normal-salt diet (0.3% NaCl, n = 6) for 13 days. Low-salt diet rats had markedly higher plasma renin activity and plasma ANG II levels. Kidney cortex renin mRNA, kidney AGT mRNA, and AGT immunoreactivity were not different; however, medullary renin mRNA, kidney renin content, and kidney ANG II levels were significantly elevated by the low-salt diet. Kidney renin immunoreactivity was also markedly increased in juxtaglomerular apparati and in cortical and medullary collecting ducts. Urinary AGT excretion rates and urinary ANG II excretion rates were not augmented by the low-salt diet. The low-salt diet caused mild renal fibrosis in glomeruli and the tubulointerstitium, but no other signs of kidney injury were evident. These results indicate that, in contrast to the response in ANG II infusion hypertension, the elevated plasma and intrarenal ANG II levels caused by physiological stimulation of RAS are not reflected by increased urinary AGT or ANG II excretion rates or the development of renal injury.

Authors+Show Affiliations

Dept. of Physiology, SL39, Tulane Univ. Health Sciences Center, 1430 Tulane Ave., New Orleans, LA 70112, USA. wshao@tulane.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

23303412

Citation

Shao, Weijian, et al. "Activation of the Renin-angiotensin System By a Low-salt Diet Does Not Augment Intratubular Angiotensinogen and Angiotensin II in Rats." American Journal of Physiology. Renal Physiology, vol. 304, no. 5, 2013, pp. F505-14.
Shao W, Seth DM, Prieto MC, et al. Activation of the renin-angiotensin system by a low-salt diet does not augment intratubular angiotensinogen and angiotensin II in rats. Am J Physiol Renal Physiol. 2013;304(5):F505-14.
Shao, W., Seth, D. M., Prieto, M. C., Kobori, H., & Navar, L. G. (2013). Activation of the renin-angiotensin system by a low-salt diet does not augment intratubular angiotensinogen and angiotensin II in rats. American Journal of Physiology. Renal Physiology, 304(5), F505-14. https://doi.org/10.1152/ajprenal.00587.2012
Shao W, et al. Activation of the Renin-angiotensin System By a Low-salt Diet Does Not Augment Intratubular Angiotensinogen and Angiotensin II in Rats. Am J Physiol Renal Physiol. 2013 Mar 1;304(5):F505-14. PubMed PMID: 23303412.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Activation of the renin-angiotensin system by a low-salt diet does not augment intratubular angiotensinogen and angiotensin II in rats. AU - Shao,Weijian, AU - Seth,Dale M, AU - Prieto,Minolfa C, AU - Kobori,Hiroyuki, AU - Navar,L Gabriel, Y1 - 2013/01/09/ PY - 2013/1/11/entrez PY - 2013/1/11/pubmed PY - 2013/5/17/medline SP - F505 EP - 14 JF - American journal of physiology. Renal physiology JO - Am. J. Physiol. Renal Physiol. VL - 304 IS - 5 N2 - In angiotensin II (ANG II) infusion hypertension, there is an augmentation of intratubular angiotensinogen (AGT) and ANG II leading to increased urinary AGT and ANG II excretion rates associated with tissue injury. However, the changes in urinary AGT and ANG II excretion rates and markers of renal injury during physiologically induced stimulation of the renin-angiotensin system (RAS) by a low-salt diet remain unclear. Male Sprague-Dawley rats received a low-salt diet (0.03% NaCl; n = 6) and normal-salt diet (0.3% NaCl, n = 6) for 13 days. Low-salt diet rats had markedly higher plasma renin activity and plasma ANG II levels. Kidney cortex renin mRNA, kidney AGT mRNA, and AGT immunoreactivity were not different; however, medullary renin mRNA, kidney renin content, and kidney ANG II levels were significantly elevated by the low-salt diet. Kidney renin immunoreactivity was also markedly increased in juxtaglomerular apparati and in cortical and medullary collecting ducts. Urinary AGT excretion rates and urinary ANG II excretion rates were not augmented by the low-salt diet. The low-salt diet caused mild renal fibrosis in glomeruli and the tubulointerstitium, but no other signs of kidney injury were evident. These results indicate that, in contrast to the response in ANG II infusion hypertension, the elevated plasma and intrarenal ANG II levels caused by physiological stimulation of RAS are not reflected by increased urinary AGT or ANG II excretion rates or the development of renal injury. SN - 1522-1466 UR - https://www.unboundmedicine.com/medline/citation/23303412/Activation_of_the_renin_angiotensin_system_by_a_low_salt_diet_does_not_augment_intratubular_angiotensinogen_and_angiotensin_II_in_rats_ L2 - http://www.physiology.org/doi/full/10.1152/ajprenal.00587.2012?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -