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(-)-Epigallocatechin-3-gallate (EGCG) attenuates peripheral nerve degeneration in rat sciatic nerve crush injury.
Neurochem Int 2013; 62(3):221-31NI

Abstract

Recently, we have shown that green tea (GT) consumption improves both reflexes and sensation in unilateral chronic constriction injury to the sciatic nerve. Considering the substantial neuroprotective properties of GT polyphenols, we sought to investigate whether (-)-epigallocatechin-3-gallate (EGCG) could protect the sciatic nerve and improve functional impairments induced by a crushing injury. We also examined whether neuronal cell apoptosis induced by the crushing injury is affected by EGCG treatment. Histological examination of sciatic nerves from EGCG-treated (50mg/kg; i.p.) showed that axonotmized rats had a remarkable axonal and myelin regeneration with significant decrease in the number of myelinated axonal fibers compared to vehicle-treated crush group. Similarly, ultrastructural evaluation of EGCG-treated nerves displayed normal unmyelinated and myelinated axons with regular myelin sheath thickness and normalized appearance of Schmidt-Lantermann clefts. Extracellular matrix displayed normal collagen fibers appearance with distinctively organized distribution similar to sham animals. Analysis of foot position and extensor postural thrust test showed a progressive and faster recovery in the EGCG-treated group compared to vehicle-treated animals. EGCG-treated rats showed significant increase in paw withdrawal thresholds to mechanical stimulation compared to vehicle-treated crush group. EGCG treatment also restored the mRNA expression of Bax, Bcl-2 and survivin but not that of p53 to sham levels on days 3 and 7 post-injury. Our results demonstrate that EGCG treatment enhanced functional recovery, advanced morphological nerve rescue and accelerated nerve regeneration following crush injury partly due to the down regulation of apoptosis related genes.

Authors+Show Affiliations

Department of Anatomy, Faculty of Medicine, Kuwait University, Kuwait. wrenno@hsc.edu.kwNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23313191

Citation

Renno, Waleed M., et al. "(-)-Epigallocatechin-3-gallate (EGCG) Attenuates Peripheral Nerve Degeneration in Rat Sciatic Nerve Crush Injury." Neurochemistry International, vol. 62, no. 3, 2013, pp. 221-31.
Renno WM, Al-Maghrebi M, Alshammari A, et al. (-)-Epigallocatechin-3-gallate (EGCG) attenuates peripheral nerve degeneration in rat sciatic nerve crush injury. Neurochem Int. 2013;62(3):221-31.
Renno, W. M., Al-Maghrebi, M., Alshammari, A., & George, P. (2013). (-)-Epigallocatechin-3-gallate (EGCG) attenuates peripheral nerve degeneration in rat sciatic nerve crush injury. Neurochemistry International, 62(3), pp. 221-31. doi:10.1016/j.neuint.2012.12.018.
Renno WM, et al. (-)-Epigallocatechin-3-gallate (EGCG) Attenuates Peripheral Nerve Degeneration in Rat Sciatic Nerve Crush Injury. Neurochem Int. 2013;62(3):221-31. PubMed PMID: 23313191.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - (-)-Epigallocatechin-3-gallate (EGCG) attenuates peripheral nerve degeneration in rat sciatic nerve crush injury. AU - Renno,Waleed M, AU - Al-Maghrebi,May, AU - Alshammari,Ahmad, AU - George,Preethi, Y1 - 2013/01/08/ PY - 2012/10/12/received PY - 2012/11/28/revised PY - 2012/12/22/accepted PY - 2013/1/15/entrez PY - 2013/1/15/pubmed PY - 2013/8/30/medline SP - 221 EP - 31 JF - Neurochemistry international JO - Neurochem. Int. VL - 62 IS - 3 N2 - Recently, we have shown that green tea (GT) consumption improves both reflexes and sensation in unilateral chronic constriction injury to the sciatic nerve. Considering the substantial neuroprotective properties of GT polyphenols, we sought to investigate whether (-)-epigallocatechin-3-gallate (EGCG) could protect the sciatic nerve and improve functional impairments induced by a crushing injury. We also examined whether neuronal cell apoptosis induced by the crushing injury is affected by EGCG treatment. Histological examination of sciatic nerves from EGCG-treated (50mg/kg; i.p.) showed that axonotmized rats had a remarkable axonal and myelin regeneration with significant decrease in the number of myelinated axonal fibers compared to vehicle-treated crush group. Similarly, ultrastructural evaluation of EGCG-treated nerves displayed normal unmyelinated and myelinated axons with regular myelin sheath thickness and normalized appearance of Schmidt-Lantermann clefts. Extracellular matrix displayed normal collagen fibers appearance with distinctively organized distribution similar to sham animals. Analysis of foot position and extensor postural thrust test showed a progressive and faster recovery in the EGCG-treated group compared to vehicle-treated animals. EGCG-treated rats showed significant increase in paw withdrawal thresholds to mechanical stimulation compared to vehicle-treated crush group. EGCG treatment also restored the mRNA expression of Bax, Bcl-2 and survivin but not that of p53 to sham levels on days 3 and 7 post-injury. Our results demonstrate that EGCG treatment enhanced functional recovery, advanced morphological nerve rescue and accelerated nerve regeneration following crush injury partly due to the down regulation of apoptosis related genes. SN - 1872-9754 UR - https://www.unboundmedicine.com/medline/citation/23313191/____Epigallocatechin_3_gallate__EGCG__attenuates_peripheral_nerve_degeneration_in_rat_sciatic_nerve_crush_injury_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0197-0186(13)00002-8 DB - PRIME DP - Unbound Medicine ER -