TY - JOUR T1 - Vasorelaxant effects of 1-nitro-2-phenylethane, the main constituent of the essential oil of Aniba canelilla, in superior mesenteric arteries from spontaneously hypertensive rats. AU - Interaminense,Leylliane de Fátima Leal, AU - dos Ramos-Alves,Fernanda Elizabethe, AU - de Siqueira,Rodrigo José Bezerra, AU - Xavier,Fabiano Elias, AU - Duarte,Gloria Pinto, AU - Magalhães,Pedro Jorge Caldas, AU - Maia,José Guilherme Soares, AU - Sousa,Pergentino José da Cunha, AU - Lahlou,Saad, Y1 - 2013/01/10/ PY - 2012/08/24/received PY - 2012/12/25/revised PY - 2012/12/30/accepted PY - 2013/1/15/entrez PY - 2013/1/15/pubmed PY - 2013/8/30/medline SP - 709 EP - 16 JF - European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences JO - Eur J Pharm Sci VL - 48 IS - 4-5 N2 - The present study investigated the mechanisms underlying the vasorelaxant effects of the essential oil of Aniba canelilla (EOAC) and its main constituent 1-nitro-2-phenylethane (NP) in isolated superior mesenteric artery from spontaneously hypertensive rats (SHRs). At 0.1-1000 μg/mL, EOAC and NP relaxed SMA preparations pre-contracted with 75 mMKCl with IC(50) (geometric mean [95% confidence interval]) values of 294.19 [158.20-94.64] and 501.27 [378.60-624.00] μg/mL, respectively); or with phenylephrine (PHE) (IC(50)s=11.07 [6.40-15.68] and 7.91 [4.08-11.74) μg/mL, respectively). All these effects were reversible and remained unaltered by vascular endothelium removal. In preparations maintained under Ca(2+)-free conditions, EOAC and NP (both at 600 μg/mL) reduced the PHE-, but not the caffeine-induced contraction. In Ca(2+)-free and high K(+) (75 mM) medium, the contractions produced by CaCl(2) or BaCl(2) were reduced or even abolished by EOAC and NP at 100 and 600 μg/mL, respectively. EOAC and NP (both at 10-1000 μg/mL) also relaxed the contraction evoked by phorbol dibutyrate (IC(50)=52.66 [10.82-94.64] and 39.13 [31.55-46.72] μg/mL, respectively). It is concluded that NP has a myogenic endothelium-independent vasorelaxant effects and appears to be the active principle of the EOAC. Vasorelaxant effect induced by both EOAC and NP is preferential to receptor-activated pathways and it appears to occur intracellularly more than a superficial action restricted to the membrane environment such as a simple blocking activity on a given receptor or ion channel. SN - 1879-0720 UR - https://www.unboundmedicine.com/medline/citation/23313621/Vasorelaxant_effects_of_1_nitro_2_phenylethane_the_main_constituent_of_the_essential_oil_of_Aniba_canelilla_in_superior_mesenteric_arteries_from_spontaneously_hypertensive_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0928-0987(13)00027-4 DB - PRIME DP - Unbound Medicine ER -